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EC number: 247-304-1
CAS number: 25869-00-5
Ammonium iron(3+) hexakis(cyano-C) ferrate(4-) was examined in a
Local Lymph Node Assay in mice according to OECD 429 and GLP
(Török-Bathó, 2011). The test substance was diluted with
dimethylformamide (DMF) to gain concentrations of 10, 25 and 50% (w/v)
that were used in the assay. A preliminiary screening test suggested
that these concentrations would not induce systemic toxicity or
excessive local irritation. The mice were treated by topical application
of the appropriate test concentrations to the dorsal surface of each ear
for 3 consecutive days (Days 1-3). On Days 4 and 5, mice were not
treated. On Day 6, all mice were injected with3H-methyl
thymidine (³HTdR) and sacrificed 5
h afterwards. The draining auricular lymph nodes were excised and pooled
for each experimental group. ³HTdR incorporation in lymphocytes was
measured by β-scintillation
counting. No deaths occurred during the study period and no
signs of systemic toxicity or local skin irritation were noted.
Stimulation indices (SI) of 1.3, 1.2 and 1.2 were calculated for the
test concentrations of 10, 25 and 50%, respectively.
Thus, under the test conditions, the test substance was not
considered to be a skin sensitizer, since it did not elicit a SI ≥ 3 in
In addition, there is a second skin sensitisation study, a Guinea Pig
Maximisation Test, available for the submission substance (Iron Blue),
performed prior to the invention of GLP and adoption of OECD guideline
406, but according to the standards at the time and with only minor
deviations from guideline protocols, which serves as supporting study
(Cuthbert and McGregor, 1977).
Migrated from Short description of key information:
Skin sensitization (Local lymph node assay, OECD 429): not sensitizing
Justification for selection of skin sensitisation endpoint:
There are two studies available for ammonium iron(3+) hexakis(cyano-C) ferrate(4-). The selected study is the most adequate and reliable study based on overall assessment of quality.
No data available.
The available data on skin sensitization of the test substance do not
meet the criteria for classification according to Regulation (EC)
1272/2008 or Directive 67/548/EEC, and are therefore conclusive but not
sufficient for classification.
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