Nicotine

Density

Link to relevant study record(s)

Reference

Endpoint:

relative density

Remarks:

Other density types indicated (migrated information): density

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2014-09-22 to 2014-09-24

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: The study was performed in accordance with standard test protocols (OECD 109 resp. EU A.3) in a quality controlled laboratory. The study is valid according to criteria mentioned in the test protocols.

Qualifier:

according to guideline

Guideline:

OECD Guideline 109 (Density of Liquids and Solids)

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method A.3 (Relative Density)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Type of method:

pycnometer method

Type:

density

Density:

1.01 g/cm³

Temp.:

20 °C

Type:

relative density

Density:

1.01

Temp.:

20 °C

Results of the single determinations with the respective error:

Determination	Density [g/cm^3]	Error of Determination [g/cm^3]
1	1.0097	0.0001
2	1.0100	0.0001

The density of the test item nicotine was calculated from

ρ = [(m₃– m₁) / (m₂– m₁)] · ρ_H2O

with

ρ	density of test item to be calculated [g/cm^3]
m1	mass of empty pycnometer [g]
m2	mass of pycnometer filled with water [g]
m3	mass of pycnometer filled with test item [g]
ρH2O	density of water at test temperature (0.9982 g/cm^3at 20.2°C)

The error of each determination method was calculated from Δρ= (2·Δm·100%) / (m₃– m₁) + (2·Δm·100%) / (m₂– m₁) with

Δρ	relative error of measured density
Δm	absolute error of single weighing
m1	mass of empty pycnometer [g]
m2	mass of pycnometer filled with water [g]
m3	mass of pycnometer filled with test item [g]

Mean values and standard deviations of the determined parameters:

Parameter	Value	Unit	Standard Deviation	Relative Standard Deviation
volume of pycnometer	10.81	cm^3	0.0028	0.03 %
density of test item nicotine	1.0099	g/cm^3	0.0002	0.02 %
density of test item nicotine	1009.9	kg/m^3	0.2	0.02 %
relative density of test item nicotine	1.0099		0.0002	0.02 %

Conclusions:

The density of nicotine corresponds to 1.0099 ± 0.0002 g/cm³ at 20.0 ± 0.4 °C. This value is the mean of two independent determinations ± the standard deviation. The standard deviation shows good correlation of the two determinations.
Therefore, the study is considered valid.

Executive summary:

The density of nicotine has been determined by the pycnometer method and corresponds to

1.0099 ± 0.0002 g/cm³at 20.0 ± 0.4 °C.

Description of key information

Study was conducted in a quality controlled laboratory. The results are reliable without restriction. Key study is appropriate for the risk assessment.

Key value for chemical safety assessment

Relative density at 20C:

1.01