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EC number: 231-595-7 | CAS number: 7647-01-0
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro DNA damage and/or repair study
- Remarks:
- Type of genotoxicity: other: mitotic recombination
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Not specified; published in 1988
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Actual concentrations tested and number of replicates are not reported, results of positive controls are not reported, and the test was not repeated.
Data source
Reference
- Reference Type:
- publication
- Title:
- Unnamed
- Year:
- 1 988
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- Deviations:
- not applicable
- Principles of method if other than guideline:
- No guideline was stated in the report, but the study complies with the TNsG on in vitro gene mutation and is largely compliant with OECD 481
- GLP compliance:
- no
- Type of assay:
- mitotic recombination assay with Saccharomyces cerevisiae
Test material
- Reference substance name:
- Hydrogen chloride
- EC Number:
- 231-595-7
- EC Name:
- Hydrogen chloride
- Cas Number:
- 7647-01-0
- Molecular formula:
- Cl H
- IUPAC Name:
- hydrogen chloride
- Details on test material:
-
Hydrochloric acid
Specification: Fisher Certified
Purity: 95% or greater
No further details specified.
Constituent 1
Method
- Target gene:
- Not applicable.
Species / strain
- Species / strain / cell type:
- other: Saccharomyces cerevisiae strain D4
- Details on mammalian cell type (if applicable):
- Nutritional deficiencies due to heteroallelism at the adenine-2 and tryptophan-5 locus.
- Additional strain / cell type characteristics:
- not specified
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9 derived from liver of rats previously treated with Aroclor 1254.
- Test concentrations with justification for top dose:
- 0.001-5 microlitre/plate
- Vehicle / solvent:
- no data
Controls
- Untreated negative controls:
- not specified
- Negative solvent / vehicle controls:
- yes
- Remarks:
- Appropriate solvent (absolute ethanol) controls were used, but not reported.
- True negative controls:
- no
- Positive controls:
- yes
- Remarks:
- Appropriate positive controls were used, but not reported.
- Positive control substance:
- not specified
- Details on test system and experimental conditions:
- Way of application: The procedure followed (Brusick and Andrews, 1974) parallels the Ames/Salmonella assay with several modifications.
Method of application: in agar (plate incorporation)
Exposure Duration: 3-5 days
Incubation temperature: 30C - S9 mix, 37C + S9 mix
Number of cells evaluated: Approximately 10^7 added to plate.
Number of replicants: not specified. - Evaluation criteria:
- The result would be considered positive for induction of mitotic recombination if the test item produced a positive dose response over three concentrations with the highest increase equal to 2-3 times the solvent control value
- Statistics:
- no data
Results and discussion
Test results
- Species / strain:
- Saccharomyces cerevisiae
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Remarks:
- with/without metabolic activation
- Cytotoxicity / choice of top concentrations:
- other: Not reported
- Vehicle controls validity:
- not specified
- Untreated negative controls validity:
- not specified
- Positive controls validity:
- not specified
- Additional information on results:
- Actual concentrations and number of replicates tested are not reported, results of positive controls are not reported, and the test was not repeated.
- Remarks on result:
- other: other: Saccharomyces cerevisiae strain D4
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
Negative results were obtained both with and without metabolic activation.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative The result would be considered positive for induction of mitotic recombination if the test item produced a positive dose response over three concentrations with the highest increase equal to 2-3 times the solvent control value
Hydrochloric acid is not mutagenic under these test conditions.
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