Registration Dossier
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EC number: 500-062-3 | CAS number: 28390-91-2
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
Description of key information
The influence of the test item 4,4’-Methylenedianiline, oligomeric reaction products with 1-chloro-2,3-epoxypropane on the growth of the freshwater green algal species Pseudokirchneriella subcapitata was investigated in a 72 hour static test according to the OECD Guideline 201 (2006) and the Commission Regulation (EC) No 761/2009, C.3.
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 11 mg/L
- EC10 or NOEC for freshwater algae:
- 0.069 mg/L
Additional information
In order to assess the toxicity of the substance containing different components to algae, water accommodated fractions (WAFs) with the loading rates of 0.32, 1.0, 3.2, 10, 32 and 100 mg/L were tested. Additionally, a control (test water without test item) group was tested in parallel. The preparation of the test media was based on the OECD Guidance Document on Aquatic Toxicity Testing of Difficult Substances and Mixtures, 2000.
For preparation of the WAFs, individual dispersions of the test item with the loading rates as mentioned above were prepared. The dispersions were ultrasonic treated for 15 minutes and continuously stirred at room temperature in the dark over 96 hours to dissolve a maximum amount of the different compounds of the test item in the dispersion. Then, the dispersions were filtered through membrane filters (0.45 µm) and the undiluted filtrates were tested as WAFs.
At the start of the test, the measured test item concentrations (based on main peak evaluation) in the test media with the loading rates of 1.0, 3.2, 10, 32 and 100 mg/L were 0.126, 0.363, 3.34, 8.84 and 18 mg/L, respectively. At the end of the test, the measured concentrations in the test media were 0.0375, 0.135, 1.29, 3.72 and 6.97 mg/L, respectively.
Evaluation of the biological results based on growth rate and related to the loading rates gave a 72-hour EL50 of > 100 mg/L, an EL20 of 48 mg/L (95% CL = 44-52 mg/L), an EL10 of 13 mg/L (95% CL = 11-15 mg/L) and a NOEL of 1.0 mg/L. Evaluation of the biological results based on growth rate and related to the mean measured concentration gave a 72-hour EC50 of > 11 mg/L, an EC20 of 6.7 mg/L (95% CL = 6.0-74 mg/L), an EC10 of 2.4 mg/L (95% CL = 1.9-2.8 mg/L) and a NOEC of 0.069 mg/L.
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