Registration Dossier
Registration Dossier
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 203-919-7 | CAS number: 111-90-0
Endpoint summary
Administrative data
Key value for chemical safety assessment
Genetic toxicity in vitro
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed (negative)
Additional information
The mutagenicity of 2 -(2 -ethoxyethoxy)ethanol has been studied in Salmonella typhimurium strains TA98, TA100, TA102, TA1535, and TA1537. Three independent experiments were conducted at 52, 162, 512, 1600 and 5000ug/plate; 492, 878, 1568, 2800 and 5000ug/plate and 10, 33, 100, 333 and 1000ug/plate respectively, with and without S9 metabolic activation systems. No signs of cytotoxicity and no precipitate were observed up to the highest dose tested, and the test article did not induce any biological relevant or statistically significant increases in the number of revertants in any of the 5 test strains, either with or without metabolic activation. Therefore, the test substance is considered non-mutagenic under the conditions of this study.
Male mice were treated intraperitoneally with 2 -(2 -ethoxyethoxy)ethanol for two consecutive days at 2ml/kg/day. A positive control group was given 100mg/kg benzopyrene. Analysis of PCE in bone marrow of animals at 48 hours and 72 hours demonstrated that the substance did not induce micronuclei whereas the number of micronuclei observed in positive control animals was increased at both time points.
In a GLP guideline study in male rats, the potential of 2 -(2 -ethoxyethoxy)ethanol to induce unschedule DNA synthesis was examined. The test substance was administered once orally by gavage at 800 or 2000mg/kg. Approximately 12-14 hours (Experiment 1) or 2-4 hours after dosing (Experiment 2), animals were killed and their livers perfused with collagenase to provide a primary culture of hepatocytes. The test substance did not product a group mean net grain count value greater than -1.7 nor were any more than 0.7% cells found in repair at either dose or experiment. Negative (vehicle) control NNG value was consistent with both published and historical control data, and the system was shown to be sensitive to two positive controls. It is concluded that 2 -(2 -ethoxyethoxy)ethanol failed to induce UDS detectable under the experimental conditions employed.
Short description of key information:
Ames tests: negative
In vivo micronucleus test: negative
In vivo liver UDS assay: negative
Endpoint Conclusion: No adverse effect observed (negative)
Justification for classification or non-classification
Data does not indicate and genotoxic effects that would warrant classification.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
