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Referenceopen allclose all

Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Study period:
December, 12-1988
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
GLP compliance:
no
Analytical monitoring:
not specified
Test organisms (species):
activated sludge
Details on inoculum:
pre-treatment: 48h of acclimatation
Test type:
static
Water media type:
freshwater
Limit test:
no
Test temperature:
25°C
pH:
7.8-8.5
Nominal and measured concentrations:
Nominal concentrations:
10000, 5000, 2500, 1250, 625 and 312.5 mg/l
Reference substance (positive control):
not specified
Duration:
3 h
Dose descriptor:
EC0
Effect conc.:
ca. 10 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate

 pH conc (mg/l) 

O2 consumption (1st) (mg/l*min)

  O2 consumption (2nd) (mg/l*min)  average  % vs control
Blw E  2.3 2.25   2.2  100
 8.5 10000   2.65  2.85  2.75  125
 8.6 5000  2.7  2.65  2.68  122
 8.5 2500   2.5  2.65  2.58  117
 8.4 1250  2.6  2.55  2.58  117
 8.1 625   2.4  2.4  2.4  109
 7.8  312.5  2.45  2.35  2.4  109
Validity criteria fulfilled:
not specified
Conclusions:
EC0 > 10 g/l
Executive summary:

the susbtance was tested for the respiration inhibition test at 25°C with a result of EC0 equal to 10 g/l.

Endpoint:
activated sludge respiration inhibition testing
Type of information:
other: read across from supporting substance
Adequacy of study:
key study
Study period:
1997
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment
Qualifier:
according to guideline
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
GLP compliance:
no
Analytical monitoring:
not specified
Test organisms (species):
activated sludge
Test type:
static
Water media type:
freshwater
Limit test:
yes
Duration:
3 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Validity criteria fulfilled:
not specified
Conclusions:
EC50 > 100 mg/l, nominal
Executive summary:

The substance was tested following OECD 209, activated sludge respiration inhibition test. The inhbition for a nominal value of the tested substance is equal to zero. The determined EC50 > 100 mg/l.

Description of key information

EC50 > 100 mg/l, nominal

Key value for chemical safety assessment

EC50 for microorganisms:
100 mg/L
EC10 or NOEC for microorganisms:
100 mg/L

Additional information

A study (Clariant, 1997) was performed on a preparation containing Acid Black 210 potassium salt. The tested substance did not show any respiration inhibition and the EC50 ( equal to NOEC) is > 100 mg/l.

Another study (BASF, 1988) tested Acid Black 210 sodium salt. The item was tested up to 10g/l without showing any respiratory inhibition with EC0 > 10g/l.

The results of the two studies are consistent and Clariant results have been chosen for CSA as the most conservative value.