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EC number: 500-234-8 | CAS number: 68891-38-3 1 - 2.5 moles ethoxylated
Long-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- fish short-term toxicity test on embryo and sac-fry stages
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- 01 Jun 2000- 11 Sep 2003
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 210 (Fish, Early-Life Stage Toxicity Test)
- Deviations:
- no
- GLP compliance:
- no
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: Water samples were collected from all test concentrations on days 0, 1, and 4, and then approximately every 4 days thereafter.
- Sampling method: not available
- Sample storage conditions before analysis: preserved with 50% methanol - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: A concentrated stock solution was prepared, which was then dispersed by the the exposure system. Deionized water was used to prepare the stock solution, and was mixed with the dilution water prior to entering the exposure chambers.
- Controls: deionized water/well water blend
- Evidence of undissolved material: No - Test organisms (species):
- Pimephales promelas
- Details on test organisms:
- Acute Exposures:
TEST ORGANISM
- Common name: Fathead minnow
- Source: In-house cultures or Aquatic Research Organisms, Hampton, NH
- Age at study initiation: 12 weeks
- Length at study initiation: avg length 34 +/- 3mm
- Weight at study initiation: wet weight 0.25 +/- 0.069 g
- Method of breeding: In-house culture
FEEDING DURING TEST
- Food type: not fed during 96 h tests
Chronic Exposures:
TEST ORGANISM
- Common name: Fathead minnow
- Source: In-house cultures or Aquatic Research Organisms, Hampton, NH
METHOD FOR PREPARATION AND COLLECTION OF FERTILIZED EGGS
- Numbers of parental fish (i.e. of females used to provide required number of eggs): no data
- Method of collection of fertilised eggs: no data
- Subsequent handling of eggs: Tests were initiated with 22-24 eggs per replicate, and thinned when eyed to no more than 16 organisms per replicate.
POST-HATCH FEEDING
- Start date: no data
- Type/source of feed: brine shrimp nauplii, then Tetra Min fish food
- Amount given: in excess
- Frequency of feeding: Daily - Test type:
- flow-through
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 28 d
- Remarks on exposure duration:
- The study (45133) included both 96 hr acute and 28 day chronic results. Acute and chronic results are detailed in separate robust summaries.
- Hardness:
- Chronic test - 165-184 mg/L. (Acute test - 200 mg/L)
- Test temperature:
- Chronic test - 23.5-26.5 deg C. (Acute test -22.2-22.7 deg C)
- pH:
- Chronic test - 7.70-7.95. (Acute test - 8.13-8.14)
- Dissolved oxygen:
- Chronic test - 7.6-7.8 mg/L. (Acute test - 7.6 mg/L)
- Nominal and measured concentrations:
- For the Chronic test, Nominal test concentrations were: 0, 0.20, 0.29, 0.41, 0.58, and 0.83 mg a.i./L. Measured concentrations were 0, 0.18, 0.29, 0.42, 0.55, and 0.80 mg/L. Measured concentrations were 90-102% of nominal. Results are based on measured concentrations.
For the Acute test, Nominal test concentrations were: 0, 0.75, 1.5, 3.0, 6.0, and 12 mg a.i./L. Measured concentrations were 0, 0.64, 1.6, 3.3, 6.6, and 13 mg/L. Measured concentrations were 85-110% of nominal. Results are based on measured concentrations. - Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Type: open
- Material: 1 or 2 L beakers depending on size of fish, with 500 um Nitex mesh covered notch to allow flow-through
- Aeration: no (test was flow-through)
- Type of flow-through: continuous, regulated by flow controllers
- Renewal rate of test solution: 41-76 volume additions per exposure chamber, depending on test
- No. of fertilized eggs/embryos per vessel: 22-24 eggs initially per replicate for chronic tests, thinned when eyed to no more than 16 per replicate. (10 fish per replicate for acute tests)
- No. of vessels per concentration (replicates): 4 for chronic test (2 for acute test)
- No. of vessels per control (replicates): 4 for chronic test (2 for acute test)
- Biomass loading rate: not specified
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Laboratory blended water (a mixture of well water and deionized water)
- Total organic carbon: N/A
- Particulate matter: N/A
- Metals: N/A
- Pesticides: N/A
- Chlorine: N/A
- Alkalinity: Chronic test - 107-113 mg/L CaCO3 mg/L. (Acute test - 140-144 mg/L CaCO3)
- Ca/mg ratio: N/A
- Conductivity: Chronic test - 380-400 umhos/cm. (Acute test - 440 umhos/cm)
- Culture medium different from test medium: no
- Intervals of water quality measurement: Daily
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 h light/ 8 h dark
EFFECT PARAMETERS MEASURED: Mortality, daily
RANGE-FINDING STUDY: no - Reference substance (positive control):
- no
- Duration:
- 28 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.18 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- act. ingr.
- Basis for effect:
- mortality
- Duration:
- 28 d
- Dose descriptor:
- EC10
- Effect conc.:
- 0.28 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- act. ingr.
- Basis for effect:
- mortality
- Remarks on result:
- other: 95% CI 0.065-0.38 mg/L
- Duration:
- 28 d
- Dose descriptor:
- other: EC20
- Effect conc.:
- 0.4 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- act. ingr.
- Basis for effect:
- mortality
- Remarks on result:
- other: 95% CI 0.26- 0.49 mg/L
- Duration:
- 28 d
- Dose descriptor:
- EC50
- Effect conc.:
- 0.63 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- act. ingr.
- Basis for effect:
- mortality
- Remarks on result:
- other: 95% CI 0.53-0.79 mg/L
- Details on results:
- The 96 hour LC50 (acute test) was 1.7 mg/L (1.5-2.1).
- Reported statistics and error estimates:
- The LC50 values and associated 95% confidence intervals were estimated using the trimmed Spearman-Karber Method or the Binomial Probability Method. EC values and associated 95% confidence intervals were estimated using Probit. Differences between replicates for exposure concentration and growth data were analyzed using ANOVA with Dunnett's Multiple Range Test. ANOVA with the Wilcoxon Two Sample Test or Dunnett's were used for survival hypothesis testing and growth analysis. All statistics except Spearman-Karber were conducted with SAS 8.01. Statistical significance was established at p<0.05.
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 28 day EC20 of C14E2S (tetradecyl dioxyethylene sulfate, AES) to fathead minnows was 0.40 mg/L, based on measured concentrations. The 28 day NOEC was 0.18 mg/L. Both results are based on survival.
- Executive summary:
The 28 -day chronic toxicity of C14E2S (tetradecyl dioxyethylene sulfate, AES) to Fathead minnows was determined in a flow-through early life-stage test. Nominal test concentrations were 0, 0.20, 0.29, 0.41, 0.58, and 0.83 mg a.i./L. Measured concentrations were 90 -102% of nominal, and results are based on measured concentrations.
There were four replicates, each containing about 15 fish, for each test concentration. Chronic exposures were initiated with 24 eggs per replicate, which were thinned when eyed. Mortality was monitored daily. Water samples were taken on days 0, 1, and 4, and then every 4 days to the end of the 33 day chronic test.
The 28 -day EC20 of C14E2S to the Fathead minnow was 0.40 mg/L (0.26 -0.49). The 28 day NOEC was 0.18 mg/L.
- Endpoint:
- long-term toxicity to fish, other
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- 14 Sep - 28 Oct 1979
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: flow-through study, analytical measurement of test concentration, some details lacking in study report
- Qualifier:
- according to guideline
- Guideline:
- other: OECD Guideline 203 (Fish, Acute Toxicity Test) Extended Acute toxicity test 45 d
- Deviations:
- not specified
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 210 (Fish, Early-Life Stage Toxicity Test)
- Deviations:
- not specified
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 215 (Fish, Juvenile Growth Test)
- Deviations:
- not specified
- GLP compliance:
- no
- Remarks:
- study pre-dates GLPs
- Analytical monitoring:
- yes
- Details on sampling:
- Not available in the study report.
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: According to standard ESD practices. Solutions were metered to the diluters by a Manostat Cassette pump Delivery System.
- Controls: Prepared using dilution water
- Evidence of undissolved material: No - Test organisms (species):
- Pimephales promelas
- Details on test organisms:
- TEST ORGANISM
- Common name: Fathead minnow
- Strain: Not available
- Source: ESD Lab culture
- Age at study initiation: 4 days
- Length at study initiation: Initial 0.4 cm (end of test 2.02 cm)
- Weight at study initiation: Initial 0.0002 g (end of test 0.093 g)
- Method of breeding: Not available
- Feeding during test: Fed
- Food type: Not available
- Amount: Not available
- Frequency: Daily
ACCLIMATION
- Acclimation period: According to standard ESD practices
- Acclimation conditions (same as test or not): Not available
- Type and amount of food: Not available
- Feeding frequency: Not available
- Health during acclimation (any mortality observed): Not available - Test type:
- flow-through
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 45 d
- Hardness:
- Hardness was measured on alternate days in the low and high test concentrations. Mean: 145 mg/L (Range 128- 174 mg/L)
- Test temperature:
- Test temperature was measured on alternate days in the low and high test concentrations. Mean temperature was 19.4 degree C. Temperature ranged 18.9- 21.1 degree C.
- pH:
- pH was measured on alternate days in the low and high test concentrations. Mean pH was 7.4 (pH ranged 6.9- 8.4).
- Dissolved oxygen:
- DO concentration was measured on alternate days in the low and high test concentrations. Mean DO was 8.2 mg/L (DO ranged 7.6- 8.6 mg/L).
- Nominal and measured concentrations:
- Nominal test concentrations based on active ingredient were 0, 0.12, 0.25, 0.5, 1 and 2 mg/L. Measured concentrations were 85% of nominal (0, 0.10, 0.21, 0.42, 0.85, and 1.70). The details of measured concentrations are in the "any other information on materials and methods" section below.
Results are based on nominal concentrations. Results are also presented on the basis of measured concentrations.. - Details on test conditions:
- TEST SYSTEM
- Test vessel Material: 20 L glass aquaria
- Type: open
- Aeration: Not available (test was flow-through)
- Type of flow-through: Proportional diluter
- Renewal rate of test solution: not provided in the study report
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 1 fish/2 L
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: dilution water was "blended" water, a mixture of well water and deionized water. ( According to ESD standards)
- Total organic carbon: Not available
- Particulate matter: Not available
- Metals: Not available
- Pesticides: Not available
- Chlorine: Not available
- Alkalinity: Not available
- Ca/mg ratio: Not available
- Conductivity: Not available
- Culture medium different from test medium: No
- Intervals of water quality measurement: Dissolved oxygen, pH, total hardness and water temperature were determined on alternate days in the high and low test concentrations.
OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: Not available
- Light intensity: Not available
EFFECT PARAMETERS MEASURED:
- Mortality of fish was monitored at 1, 3, 6 and 24hrs, and daily thereafter until termination at day 45.
- Growth of fish (length, weight) was measured at the end of the study (45 days).
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2
- Range finding study: No - Reference substance (positive control):
- no
- Duration:
- 45 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- length
- Duration:
- 45 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- weight
- Duration:
- 45 d
- Dose descriptor:
- EC10
- Effect conc.:
- 0.98 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- mortality
- Remarks on result:
- other: 95% CL: 0.94 - 1.71 mg/L
- Duration:
- 45 d
- Dose descriptor:
- LC50
- Effect conc.:
- 1.05 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- mortality
- Remarks on result:
- other: 95% CL: 1.00 - 1.83 mg/L
- Details on results:
- - Behavioural abnormalities: None
- Observations on body length and weight: See "details on test organisms" above.
- Other biological observations: None
- Mortality of control: 1/10 (1 control fish expired on day 5)
- Other adverse effects control: None
- Abnormal responses: None
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: No
- Effect concentrations exceeding solubility of substance in test medium: No - Reported statistics and error estimates:
- All statistical analyses were performed in R, version 2.11.1. Weight and length values were analyzed by ANOVA, and NOEC levels for each were estimated by pairwise comparisons to control through the multcomp package. For mortality, pairwise comparisons to control were made by Fisher's Exact test, with p-value adjustments by the method of Holm to estimate NOEC levels. The EC10 and EC50 values (based on mortality) were estimated by a logistic regression model incorporating Abbott's correction for background mortality (Hoekstra, 1987).
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 45 day EC10 (based on mortality) of C12-14EO2.25S (alkylethoxysulfate, AES) to Fathead minnow fry was 0.98 mg/L, based on nominal test concentrations in a flow-through test. The 45 day NOEC (based on length, weight) was 1.0 mg/L. Measured concentrations were 85% of nominal.
- Executive summary:
In a 45 day chronic toxicity study, Fathead minnow fry (Pimephales promelas) were exposed to C14 -16EO2.25S (alkyl ethoxy sulfate, AES) in a flow-through test. Nominal test concentrations were 0, 0.12, 0.25, 0.5, 1, and 2 mg ai/L. Measured test concentrations were 85% of nominal (MBAS method). Results are based on nominal concentrations.
The 45 day EC10 was 0.98 mg/L, based on mortality. The 45 day NOEC was 1.0 mg/L, based on growth (length, weight). The 45 day LC50 was 1.05 mg/L.
- Endpoint:
- long-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- 14 Sep -28 Oct 1979
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: flow-through study, analytical measurement of test concentration, some details lacking in study report
- Qualifier:
- according to guideline
- Guideline:
- other: OECD Guideline 203 (Fish, Acute Toxicity Test) Extended Acute toxicity test 45 d
- Deviations:
- not specified
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 210 (Fish, Early-Life Stage Toxicity Test)
- Deviations:
- not specified
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 215 (Fish, Juvenile Growth Test)
- Deviations:
- not specified
- GLP compliance:
- no
- Remarks:
- study pre-dates GLPs
- Analytical monitoring:
- yes
- Details on sampling:
- Not available in the study report.
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: According to standard ESD practices. Solutions were metered to the diluters by a Manostat Cassette pump Delivery System.
- Controls: Prepared using dilution water
- Evidence of undissolved material: No - Test organisms (species):
- Pimephales promelas
- Details on test organisms:
- TEST ORGANISM
- Common name: Fathead minnow
- Strain: Not available
- Source: Dale Fattig, Brady, Nebraska
- Age at study initiation: 9 months old
- Length at study initiation: Initial 3.6 cm (end of test 3.9 cm)
- Weight at study initiation: Initial 0.77 g (end of test 0.97 g)
- Method of breeding: Not available
- Feeding during test: Fed
- Food type: Not available
- Amount: Not available
- Frequency: Daily
ACCLIMATION
- Acclimation period: According to standard ESD practices
- Acclimation conditions (same as test or not): Not available
- Type and amount of food: Not available
- Feeding frequency: Not available
- Health during acclimation (any mortality observed): Not available - Test type:
- flow-through
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 45 d
- Hardness:
- Hardness was measured on alternate days in the low and high test concentrations. Mean: 150 mg/L (Range 131- 209 mg/L)
- Test temperature:
- Test temperature was measured on alternate days in the low and high test concentrations. Mean temperature was 19.5 degree C. Temperature ranged 18.9- 21.1 degree C.
- pH:
- pH was measured on alternate days in the low and high test concentrations. Mean pH was 7.8 (pH ranged 7.7-7.9).
- Dissolved oxygen:
- DO concentration was measured on alternate days in the low and high test concentrations. Mean DO was 7.9 mg/L (DO ranged 5.4- 8.4 mg/L).
- Nominal and measured concentrations:
- Nominal test concentrations based on active ingredient were 0, 0.12, 0.25, 0.5, 1 and 2 mg/L. Measured concentrations were 82% of nominal (0, 0.10, 0.21, 0.41, 0.82, and 1.65). The details of measured concentrations are in the "any other information on materials and methods" section below.
Results are based on nominal concentrations. Results are also presented on the basis of measured concentrations.. - Details on test conditions:
- TEST SYSTEM
- Test vessel Material: 20 L glass aquaria
- Type: open
- Aeration: Not available (test was flow-through)
- Type of flow-through: Proportional diluter
- Renewal rate of test solution: not provided in the study report
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 1 fish/2 L
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: dilution water was "blended" water, a mixture of well water and deionized water. ( According to ESD standards)
- Total organic carbon: Not available
- Particulate matter: Not available
- Metals: Not available
- Pesticides: Not available
- Chlorine: Not available
- Alkalinity: Not available
- Ca/mg ratio: Not available
- Conductivity: Not available
- Culture medium different from test medium: No
- Intervals of water quality measurement: Dissolved oxygen, pH, total hardness and water temperature were determined on alternate days in the high and low test concentrations.
OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: Not available
- Light intensity: Not available
EFFECT PARAMETERS MEASURED:
- Mortality of fish was monitored at 1, 3, 6 and 24hrs, and daily thereafter until termination at day 45.
- Growth of fish (length, weight) was measured at the end of the study (45 days).
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2
- Range finding study: No - Reference substance (positive control):
- no
- Duration:
- 45 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- length
- Duration:
- 45 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- weight
- Duration:
- 45 d
- Dose descriptor:
- EC10
- Effect conc.:
- 0.67 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- mortality
- Remarks on result:
- other: 95% CL: 0.54-0.80 mg/L
- Duration:
- 45 d
- Dose descriptor:
- LC50
- Effect conc.:
- 0.9 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- mortality
- Remarks on result:
- other: 95% CL: 0.73-1.08 mg/L
- Details on results:
- - Behavioural abnormalities: None
- Observations on body length and weight: See "details on test organisms" above.
- Other biological observations: None
- Mortality of control: none
- Other adverse effects control: None
- Abnormal responses: None
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: No
- Effect concentrations exceeding solubility of substance in test medium: No - Reported statistics and error estimates:
- All statistical analyses were performed in R, version 2.11.1. Weight and length values were analyzed by ANOVA, and NOEC levels for each were estimated by pairwise comparisons to control through the multcomp package. For mortality, pairwise comparisons to control were made by Fisher's Exact test, with p-value adjustments by the method of Holm to estimate NOEC levels. The EC10 and EC50 values (based on mortality) were estimated by a logistic regression model incorporating Abbott's correction for background mortality (Hoekstra, 1987).
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 45 day EC10 (based on mortality) of C12-14EO2.25S (alkylethoxysulfate, AES) to Fathead minnow juveniles was 0.67 mg/L, based on nominal test concentrations in a flow-through test. The 45 day NOEC (based on length, weight) was 1.0 mg/L. Measured concentrations were 82% of nominal.
- Executive summary:
In a 45 day chronic toxicity study, Fathead minnow juveniles (Pimephales promelas) were exposed to C14 -16EO2.25S (alkyl ethoxy sulfate, AES) in a flow-through test. Nominal test concentrations were 0, 0.12, 0.25, 0.5, 1, and 2 mg ai/L. Measured test concentrations were 82% of nominal (MBAS method). Results are based on nominal concentrations.
The 45 day EC10 was 0.67 mg/L, based on mortality. The 45 day NOEC was 1.0 mg/L, based on growth (length, weight). The 45 day LC50 was 0.90 mg/L.
Referenceopen allclose all
Mortality of Fathead Minnow Fry in 45 Day test of AES (study 23595) |
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Time |
Mortalities (10 fish per nominal concentration; mg/L) |
||||||||||||||||
Hours |
Day |
Control |
0.12 |
0.25 |
0.5 |
1 |
2 |
||||||||||
1 |
|
0 |
0 |
0 |
0 |
0 |
0 |
||||||||||
3 |
|
0 |
0 |
0 |
0 |
0 |
0 |
||||||||||
6 |
|
0 |
0 |
0 |
0 |
0 |
9 |
||||||||||
|
1 |
0 |
0 |
0 |
0 |
1 |
1 |
||||||||||
|
2 |
0 |
0 |
0 |
0 |
0 |
0 |
||||||||||
|
3 |
0 |
0 |
0 |
0 |
2 |
0 |
||||||||||
|
4 |
0 |
0 |
0 |
0 |
0 |
0 |
||||||||||
|
5 |
1 |
0 |
0 |
0 |
1 |
0 |
||||||||||
|
6 |
0 |
0 |
0 |
0 |
1 |
0 |
||||||||||
|
7 |
0 |
0 |
0 |
0 |
0 |
0 |
||||||||||
|
8 |
0 |
0 |
0 |
0 |
0 |
0 |
||||||||||
|
9 |
0 |
0 |
0 |
0 |
0 |
0 |
||||||||||
|
10 |
0 |
0 |
0 |
0 |
0 |
0 |
||||||||||
|
11 |
0 |
0 |
0 |
0 |
1 |
0 |
||||||||||
|
12 |
0 |
0 |
0 |
0 |
0 |
0 |
||||||||||
|
13 |
0 |
0 |
0 |
0 |
0 |
0 |
||||||||||
|
14 |
0 |
0 |
0 |
0 |
0 |
0 |
||||||||||
|
15 |
0 |
0 |
0 |
0 |
0 |
0 |
||||||||||
|
16 |
0 |
0 |
0 |
1 |
0 |
0 |
||||||||||
|
17 |
0 |
0 |
0 |
0 |
0 |
0 |
||||||||||
|
18 |
0 |
0 |
0 |
0 |
0 |
0 |
||||||||||
|
19 |
0 |
0 |
1 |
0 |
0 |
0 |
||||||||||
|
20 |
0 |
0 |
0 |
0 |
0 |
0 |
||||||||||
|
21 |
0 |
0 |
0 |
0 |
0 |
0 |
||||||||||
|
22 |
0 |
0 |
0 |
0 |
0 |
0 |
||||||||||
|
23 |
0 |
0 |
0 |
0 |
1 |
0 |
||||||||||
|
24 |
0 |
0 |
0 |
0 |
0 |
0 |
||||||||||
|
25 |
0 |
0 |
0 |
0 |
0 |
0 |
||||||||||
|
26 |
0 |
0 |
0 |
0 |
0 |
0 |
||||||||||
|
27 |
0 |
0 |
0 |
0 |
0 |
0 |
||||||||||
|
28 |
0 |
0 |
0 |
0 |
0 |
0 |
||||||||||
|
29 |
0 |
0 |
0 |
0 |
0 |
0 |
||||||||||
|
30 |
0 |
0 |
0 |
0 |
0 |
0 |
||||||||||
|
31 |
0 |
0 |
0 |
0 |
0 |
0 |
||||||||||
|
32 |
0 |
0 |
0 |
0 |
0 |
0 |
||||||||||
|
33 |
0 |
0 |
0 |
0 |
0 |
0 |
||||||||||
|
34 |
0 |
0 |
0 |
0 |
0 |
0 |
||||||||||
|
35 |
0 |
0 |
0 |
0 |
0 |
0 |
||||||||||
|
36 |
0 |
0 |
0 |
0 |
0 |
0 |
||||||||||
|
37 |
0 |
0 |
0 |
0 |
0 |
0 |
||||||||||
|
38 |
0 |
0 |
0 |
0 |
0 |
0 |
||||||||||
|
39 |
0 |
0 |
0 |
0 |
0 |
0 |
||||||||||
|
40 |
0 |
0 |
0 |
0 |
0 |
0 |
||||||||||
|
41 |
0 |
0 |
0 |
0 |
0 |
0 |
||||||||||
|
42 |
0 |
0 |
0 |
0 |
0 |
0 |
||||||||||
|
43 |
0 |
0 |
0 |
0 |
0 |
0 |
||||||||||
|
44 |
0 |
0 |
0 |
0 |
0 |
0 |
||||||||||
|
45 |
0 |
0 |
0 |
0 |
0 |
0 |
||||||||||
|
|
|
|
|
|
|
|
||||||||||
Cumulative mortality (45 days) |
1 |
0 |
1 |
1 |
7 |
10 |
|||||||||||
Notes: |
|||||||||||||||||
Day 19, 0.25 test concentration: fish was missing and assumed dead. Replacement fish did not die in subsequent 26 days. |
|||||||||||||||||
Day 16, 0.50 test concentration: fish was missing and assumed dead. Replacement fish did not die in subsequent 29 days. |
|||||||||||||||||
Mortality of Fathead Minnow Juveniles in 45 Day test of AES (study 23596) |
|
|||||||||||||
|
||||||||||||||
Time |
Mortalities (10 fish per nominal concentration; mg/L) |
|||||||||||||
Hours |
Day |
Control |
0.12 |
0.25 |
0.5 |
1 |
2 |
|||||||
1 |
|
0 |
0 |
0 |
0 |
0 |
0 |
|||||||
3 |
|
0 |
0 |
0 |
0 |
0 |
0 |
|||||||
6 |
|
0 |
0 |
0 |
0 |
0 |
0 |
|||||||
|
1 |
0 |
0 |
0 |
0 |
0 |
5 |
|||||||
|
2 |
0 |
0 |
0 |
0 |
0 |
4 |
|||||||
|
3 |
0 |
0 |
0 |
0 |
0 |
0 |
|||||||
|
4 |
0 |
0 |
0 |
0 |
0 |
0 |
|||||||
|
5 |
0 |
0 |
0 |
0 |
0 |
0 |
|||||||
|
6 |
0 |
0 |
1 |
0 |
0 |
0 |
|||||||
|
7 |
0 |
0 |
0 |
0 |
0 |
0 |
|||||||
|
8 |
0 |
0 |
0 |
0 |
0 |
0 |
|||||||
|
9 |
0 |
0 |
0 |
0 |
0 |
0 |
|||||||
|
10 |
0 |
0 |
0 |
0 |
0 |
0 |
|||||||
|
11 |
0 |
0 |
0 |
0 |
0 |
0 |
|||||||
|
12 |
0 |
0 |
0 |
0 |
0 |
1 |
|||||||
|
13 |
0 |
0 |
0 |
0 |
1 |
0 |
|||||||
|
14 |
0 |
0 |
0 |
0 |
1 |
0 |
|||||||
|
15 |
0 |
0 |
0 |
0 |
0 |
0 |
|||||||
|
16 |
0 |
0 |
0 |
0 |
0 |
0 |
|||||||
|
17 |
0 |
0 |
0 |
0 |
0 |
0 |
|||||||
|
18 |
0 |
0 |
0 |
0 |
0 |
0 |
|||||||
|
19 |
0 |
0 |
0 |
0 |
0 |
0 |
|||||||
|
20 |
0 |
0 |
0 |
0 |
0 |
0 |
|||||||
|
21 |
0 |
0 |
0 |
0 |
0 |
0 |
|||||||
|
22 |
0 |
0 |
0 |
0 |
0 |
0 |
|||||||
|
23 |
0 |
0 |
0 |
0 |
0 |
0 |
|||||||
|
24 |
0 |
0 |
0 |
0 |
0 |
0 |
|||||||
|
25 |
0 |
0 |
0 |
0 |
0 |
0 |
|||||||
|
26 |
0 |
0 |
0 |
0 |
0 |
0 |
|||||||
|
27 |
0 |
0 |
0 |
0 |
0 |
0 |
|||||||
|
28 |
0 |
0 |
0 |
0 |
0 |
0 |
|||||||
|
29 |
0 |
0 |
0 |
0 |
0 |
0 |
|||||||
|
30 |
0 |
0 |
0 |
0 |
0 |
0 |
|||||||
|
31 |
0 |
1 |
0 |
0 |
0 |
0 |
|||||||
|
32 |
0 |
0 |
0 |
0 |
0 |
0 |
|||||||
|
33 |
0 |
0 |
0 |
0 |
0 |
0 |
|||||||
|
34 |
0 |
0 |
0 |
0 |
0 |
0 |
|||||||
|
35 |
0 |
0 |
0 |
0 |
0 |
0 |
|||||||
|
36 |
0 |
0 |
0 |
0 |
0 |
0 |
|||||||
|
37 |
0 |
0 |
0 |
0 |
0 |
0 |
|||||||
|
38 |
0 |
0 |
0 |
0 |
0 |
0 |
|||||||
|
39 |
0 |
0 |
0 |
0 |
0 |
0 |
|||||||
|
40 |
0 |
0 |
0 |
0 |
0 |
0 |
|||||||
|
41 |
0 |
0 |
0 |
0 |
0 |
0 |
|||||||
|
42 |
0 |
0 |
0 |
0 |
0 |
0 |
|||||||
|
43 |
0 |
0 |
0 |
0 |
0 |
0 |
|||||||
|
44 |
0 |
0 |
0 |
0 |
0 |
0 |
|||||||
|
45 |
0 |
0 |
0 |
0 |
0 |
0 |
|||||||
|
|
|
|
|
|
|
|
|||||||
Cumulative mortality (45 days) |
0 |
1 |
1 |
0 |
2 |
10 |
||||||||
Description of key information
NOEC (28 d) for Oncorhynchus mykiss = 0.14 mg/L (measured) related to mortality and sublethal effects (similar to OECD 215)
Key value for chemical safety assessment
Additional information
The assessment is based on the data currently available. New studies, based on the category review and the final decisions issued for some of the category substances, which are also relevant for this assessment, are currently being conducted. The hazard assessment with respect to aquatic toxicity will be updated once all ongoing studies have been finalised.
To assess the long-term toxicity of AES (C12-14, 1-2.5EO) Na (CAS 68891-38-3) one study is available. The results of that key study are supported by investigations with the pure AES homologue (C14, 2EO) Na (CAS 27731-62-0) and with the similar alcohol ethoxysulfate mixture (C14-16, 2EO) Na (CAS 68585-34-2).
To determine the toxic effect of the AES (C12-14, 2EO) Na a study similar to OECD guideline 215 was performed (TEGEWA, 1995). In this test juvenile individuals of rainbow trout (Oncorhynchus mykiss) were exposed to different concentrations of AES (C12 -14, 2EO) Na under flow-through conditions. Mortality and growth were determined over 28 days. Related to mortality and sublethal effects a NOEC of 0.14 mg/L (measured) was determined.
This result is supported by a non-GLP study carried out similar to OECD guideline 210, eggs of fathead minnow (Pimephales promelas) were exposed to the pure AES homologue (C14, 2EO) Na (P&G, 2004). Under flow-through conditions a NOEC (28 d) of 0.18 mg/L (measured) was determined. The NOEC based on mortality.
Two further studies are available for AES (C14-16, 2EO). In the first study fathead minnow fry were exposed in a flow-through system (P&G, 1979a). In the second study fathead minnow juveniles were exposed in the same way with the same mixture (P&G, 1979b). Both tests were conducted according to OECD 203 over 45 days. In both studies a NOEC for growth (length and weight) was reported to be 1.0 mg/L.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
