Alcohols, C12-14, ethoxylated, sulfates, sodium salts

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

25 - 27 Aug 1993

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Version / remarks:

1984

Qualifier:

according to guideline

Guideline:

other: EG Guideline 92/69/EWG

GLP compliance:

yes

Analytical monitoring:

yes

Details on sampling:

- Concentrations: all concentrations
- Sampling method: samples were taken at 0 h and after 48 h

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: a stock solution with a concentration of 1.43 g/L was prepared.

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: water flea
- Strain: Klon 5
- Source: in-house culture
- Age at study initiation (mean and range, SD): neonates, isolation of parent daphnids approximately 24 h prior to test start.
- Method of breeding: Daphnids are cultured in M4- medium and fed daily with Scenedesmus subspicatus
- Feeding during test: none

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test temperature:

20 +/- 1 °C

pH:

7.3 - 7.5

Dissolved oxygen:

7.2 - 8.6 mg/L

Nominal and measured concentrations:

Nominal concentration: 5, 9, 15, 26, 45, 80 and 135 mg/L
Mean measured (arith.): 4.4, 7.55, 14, 25.5, 43.5, 78.5, and 139.5 mg/L based on anionic surfactants concentration (MBAS)

Details on test conditions:

TEST SYSTEM
- Test vessel: test tubes
- Material, fill volume: glass, filled with 10 mL test medium
- Aeration: none
- No. of organisms per vessel: 5 (20 animals per concentration)
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Biomass loading rate: 2 mL per test organism


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: synthetic freshwater (M4-medium) according to Elendt
294 mg/L CaCl2 * 2 H2O
123 mg/L MgSO42 * 7 H2O
63 mg/L NaHCO3
5.5 mg/L KCl
- Ca/mg ratio: 4 : 1
- Intervals of water quality measurement: test end

OTHER TEST CONDITIONS
- Photoperiod: continous darkness

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

7.4 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

mobility

Remarks on result:

other: corresponds to a nominal concentration of 7.2 mg/L based on anionic surfactants concentration (MBAS)

Results with reference substance (positive control):

EC100 (24 h): 1.9 mg/L (test conducted on 03 Aug 1993)

Reported statistics and error estimates:

Effect concentrations were determined graphically.

Table 1 Results mobility

Concentration [mg/L]	Test duration
	24 h			48 h
	Number mobile	Number immobile	% immobile	Number mobile	Number immobile	% immobile
Control	20	0	0	20	0	0
5	20	0	0	20	0	0
9	19	1	5	4	16	80
15	7	13	65	0	20	100
26	0	20	100	0	20	100
45	0	20	100	0	20	100
80	0	20	100	0	20	100
135	0	20	100	0	20	100

Table 2 Measured concentrations based on methylene blue active substance concentration (MBAS)

Nominal concentration [mg/L]	Measured concentration [mg/L]
	0 h	48 h
control	0.09	--
5	3.8	5
9	6.3	8.8
15	13	15
26	21	30
45	37	50
80	73	84
134	129	150

Table 3: Validity criteria for OECD 202.

Criterion from the guideline	Outcome	Validity criterion fulfilled
In the control, including the control containing the solubilising agent, not more than 10% of the daphnids should have been immobilized.	0%	yes
The dissolved oxygen concentration at the end of the test should be ≥ 3 mg/L in control and test vessels.	7.2 - 8.6 mg/L	yes

 

Validity criteria fulfilled:

yes

Remarks:

The validity crietria according to OECD 202 are fulfilled (for details see table 3 under "any information on results")

Description of key information

EC50 (48 h): 7.4 mg a.i./L (nominal, OECD 202)

Additional information

One study investigating the acute toxicity of Alcohols, C12-14, ethoxylated, sulfates, sodium salts to aquatic invertebrates is available. The study was performed in a static test system and followed OECD guideline 202. Daphnia magna were exposed for 48 h nominal exposure concentrations of 5, 9, 15, 26, 45, 80 and 135 mg a.i./L.

The test was conducted with standard M4 medium. The test concentrations were analytically verified using the MBAS assay (methylene blue active substances assay) as analytic test method. Based on the anionic surfactants concentration (MBAS) the mean measured concentrations were 4.4, 7.55, 14, 25.5, 43.5, 78.5, and 139.5 mg/L. An EC50 (48 h) of 7.4 mg a.i. /L was determined (EC50 (48 h): 7.2 mg/L based on anionic surfactants concentration (MBAS)).