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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
25 - 27 Aug 1993
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study with acceptable restrictions
Qualifier:
according to guideline
Guideline:
other: EG Guideline 92/69/EWG
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Version / remarks:
1984
GLP compliance:
yes
Analytical monitoring:
yes
Details on sampling:
- Concentrations: all concentrations
- Sampling method: samples were taken at 0 h and after 48 h
Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: a stock solution with a concentration of 1.43 g/L was prepared.
Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: water flea
- Strain: Klon 5
- Source: in-house culture
- Age at study initiation (mean and range, SD): neonates, isolation of parent daphnids approximately 24 h prior to test start.
- Method of breeding: Daphnids are cultured in M4- medium and fed daily with Scenedesmus subspicatus
- Feeding during test: none
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Test temperature:
20 +/- 1 °C
pH:
7.3 - 7.5
Dissolved oxygen:
7.2 - 8.6 mg/L
Nominal and measured concentrations:
Nominal concentration: 5, 9, 15, 26, 45, 80 and 135 mg/L
Mean measured (arith.): 4.4, 7.55, 14, 25.5, 43.5, 78.5, and 139.5 mg/L based on anionic surfactants concentration (MBAS)
Details on test conditions:
TEST SYSTEM
- Test vessel: test tubes
- Material, fill volume: glass, filled with 10 mL test medium
- Aeration: none
- No. of organisms per vessel: 5 (20 animals per concentration)
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Biomass loading rate: 2 mL per test organism


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: synthetic freshwater (M4-medium) according to Elendt
294 mg/L CaCl2 * 2 H2O
123 mg/L MgSO42 * 7 H2O
63 mg/L NaHCO3
5.5 mg/L KCl
- Ca/mg ratio: 4 : 1
- Intervals of water quality measurement: test end

OTHER TEST CONDITIONS
- Photoperiod: continous darkness
Reference substance (positive control):
yes
Remarks:
potassium dichromate
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
7.4 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
mobility
Remarks on result:
other: corresponds to a nominal concentration of 7.2 mg/L based on anionic surfactants concentration (MBAS)
Results with reference substance (positive control):
EC100 (24 h): 1.9 mg/L (test conducted on 03 Aug 1993)
Reported statistics and error estimates:
Effect concentrations were determined graphically.

Table 1 Results mobility

Concentration [mg/L]

Test duration

24 h

48 h

Number mobile

Number immobile

% immobile

Number mobile

Number immobile

% immobile

Control

20

0

0

20

0

0

5

20

0

0

20

0

0

9

19

1

5

4

16

80

15

7

13

65

0

20

100

26

0

20

100

0

20

100

45

0

20

100

0

20

100

80

0

20

100

0

20

100

135

0

20

100

0

20

100

Table 2 Measured concentrations based on methylene blue active substance concentration (MBAS)

Nominal concentration [mg/L] Measured concentration [mg/L]  
0 h 48 h
control 0.09  -- 
5 3.8 5
9 6.3 8.8
15 13 15
26 21 30
45 37 50
80 73 84
134 129 150

Table 3: Validity criteria for OECD 202.

Criterion from the guideline

Outcome

Validity criterion fulfilled

In the control, including the control containing the solubilising agent, not more than 10% of the daphnids should have been immobilized.

0%

yes

The dissolved oxygen concentration at the end of the test should be ≥ 3 mg/L in control and test vessels.

7.2 - 8.6 mg/L

yes


 

Validity criteria fulfilled:
yes
Remarks:
The validity crietria according to OECD 202 are fulfilled (for details see table 3 under "any information on results")

Description of key information

In the key-study an EC50-value for daphnids of 7.4 mg a.s./L could be determined after 48 h of exposure.  

Key value for chemical safety assessment

Additional information

One study on the acute toxicity of the substance to aquatic invertebrates is available. Daphnia magna were exposed for 48 h in a static test system set up according to guidelines 92/69/EWG and OECD 202. The test was conducted with standard M4 medium. The nominal exposure concentrations were 5, 9, 15, 26, 45, 80 and 135 mg/L. The test concentrations were analytically verified using the MBAS assay (methylene blue active substances assay) as analytic test method. Based on the anionic surfactants concentration (MBAS) the mean measured concentrations were 4.4, 7.55, 14, 25.5, 43.5, 78.5, and 139.5 mg/L. An EC50 (48 h) of 7.4 mg a.i. /L was determined (EC50 (48 h): 7.2 mg/L based on anionic surfactants concentration (MBAS)).