Biphenyl-4,4'-diol

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Remarks:

GLP study conducted to international test guideline (OECD 203) with chemical analysis by HPLC

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

GLP compliance:

yes

Specific details on test material used for the study:

Biphenyl-4,4’-diol / CAS 92-88-6

Analytical monitoring:

yes

Details on sampling:

Test medium sampled at 0 hours (new) and 24 hours (old)

Vehicle:

yes

Details on test solutions:

Test solutions prepared by dissolution of weighed quantity of test substance in solvent, dimethylformamide (DMF) followed by dilution (100 ul/L) in treated water to provide final test concentrations

Test organisms (species):

Oryzias latipes

Details on test organisms:

Length: 1.55 cm (1.5 to 1.75 cm) n = 10
Weight: 0.047 g (0.030 to 0.090 g) n = 10
Loading 1 g/L

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Post exposure observation period:

NA

Hardness:

Not reported

Test temperature:

Temp: 23.7 to 24.9°C

pH:

pH : 7.3 to 7.9

Dissolved oxygen:

Dissolved oxygen: 6.7 to 8.3

Salinity:

NA

Nominal and measured concentrations:

Nominal concentrations: 3.0, 5.3, 9.5, 17 and 30 mg/L (spacing factor 1.8)
Measured concentrations: (0 hours): 3.05, 5.57, 9.56, 17.1 and 30.7 mg/L
Measured concentrations: (24 hours): 3.02, 5.60, 9.61, 16.9 and 29.7 mg/L.
Mean measured concentrations: 3.03, 5.58, 9.58, 17 and 30.2 mg/L (0-24 hours)

Details on test conditions:

Diluent and solvent (DMF) controls
1 replicate per concentration, 5.0 L and 10 fish per vessel
16 hour light:8 hour dark

1000 Lux, 24 ± 1°C

Reference substance (positive control):

no

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

12.8 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: (9.58 to 17.0 mg/L)

Key result

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

9.58 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Key result

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

17 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

During the conduct of the test there was no mortality in the solvent control, 3.0 or 9.5 mg/L groups, 10 % mortality occured in the control and 5.3 mg/L. At concentrations of 17 and 30 mg/L 100% mortality was evident.

Results with reference substance (positive control):

NA

Reported statistics and error estimates:

Binomial

Sublethal observations / clinical signs:

Observations of mortality

Nominal (mg/L)	Mean measured (mg/L)	% mortality
		24 hours	48 hours	72 hours	96 hours
Control	-	0	0	0	10
S. Control	-	0	0	0	0
3.0	3.03	0	0	0	0
5.3	5.58	0	0	0	10
9.5	9.58	0	0	0	0
17	17.0	50	100	100	100
30	30.2	100	100	100	100

Analytical results

Nominal concentration (mg/L)	Measured concentrations (mg/L)		Geometric mean measured concentrations (mg/L)
	0 hours	24 hours
Control	<LOD	<LOD	<LOD
Solvent control	<LOD	<LOD	<LOD
3.0	3.05	3.02	3.03
5.3	5.57	5.60	5.58
9.5	9.56	9.61	9.58
17	17.1	16.9	17
30	30.7	29.7	30.2

Validity criteria fulfilled:

yes

Conclusions:

At the end of the test the 96 hour LC50 was calculated to be 12.8 mg/L (CI 95% 9.58 to 17 mg/L)
The NOEC based on mortality was 9.58 mg/L
The NOEC based on sub-lethal effects was 3.03 mg/L

Executive summary:

A GLP compliant 96 hour semi-static acute toxicity Oryzias latipes (medaka) was performed in accordance with OECD 203 (1992) to assess the toxicity of 4,4,biphenol. Nominal test concentrations were : 3.0, 5.3, 9.5, 17 and 30 mg/L (spacing factor 1.8) a diluent and solvent (DMF) control were also included and measured concentrations were determined.

The geometric mean measured concentrations were 3.03, 5.58, 9.58, 17 and 30.2 mg/L. 

At the end of the test the 96 hour LC₅₀was calculated to be 12.8 mg/L (CI 95% 9.58 to 17 mg/L) based on geometric mean measured concentrations.

The NOEC based on mortality was 9.58 mg/L The NOEC based on sub-lethal effects was 3.03 mg/L (based on geometric mean measured concentrations).

Description of key information

Semi-static GLP compliant acute toxicity test with Oryzias latipes (medaka) exposed to 4,4-biphenol, conducted in accordance with OECD 203 (1992) and supporting chemical analysis.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

12.8 mg/L

Additional information

A GLP compliant 96 hour semi-static acute toxicity test with Oryzias latipes (medaka) was performed in accordance with OECD 203 (1992) to assess the toxicity of biphenyl-4,4'-diol. Nominal test concentrations were : 3.0, 5.3, 9.5, 17 and 30 mg/L (spacing factor 1.8). A diluent and solvent (DMF) control were also included and measured concentrations were determined.

Following analytical determination the geometric mean measured concentrations were 3.03, 5.58, 9.58, 17 and 30.2 mg/L. The results below are based on geometric mean measured concentrations.

At the end of the test the 96 hour LC50was calculated to be 12.8 mg/L (CI 95% 9.58 to 17 mg/L).

The NOEC based on mortality was 9.58 mg/L. The NOEC based on sub-lethal effects was 3.03 mg/L.