Dodecylamine

Long-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

long-term toxicity to aquatic invertebrates

Type of information:

migrated information: read-across based on grouping of substances (category approach)

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Guideline study with acceptable restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2002

Report date:

2002

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 211 (Daphnia magna Reproduction Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Amines, coco alkyl

EC Number:

262-977-1

EC Name:

Amines, coco alkyl

Cas Number:

61788-46-3

IUPAC Name:

61788-46-3

Details on test material:

- Name of test material (as cited in study report): Cocoalkylamine

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Dispersion treatment: Stock solution was 10 mg/L. The test item was applied to natural river water. This solution was treated for 30 minutes with ultrasound at 40 °C.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Water flea
- Strain: Daphnia magna STRAUS (Clone 5)
- Source: Institut für Wasser-, Boden- und Lufthygiene (WaBoLu); Breeder DR.U.NOACK-LABORATORIEN, D-31157 Sarstedt, Germany
- Age at study initiation: 2 - 24 h old
- Feeding during test: The daphnids were not fed during the study.

HOUSING:
- Conditions: In 2-3 L glass vessels with approximately 1.8 L culture medium, at 21 °C (temperatures of 20 - 25 °C are tolerated), in an
incubator, 16 h illumination, illumination strength max. 20 μE⋅m-2 ⋅s-1.
- Culture medium: Elendt M4, according to ELENDT (1990), modified to a total hardness of 160 to 180 mg CaCO3/L.
- Type and amount of food: Feeding ad libitum with a mix of Desmodesmus subspicatus and Chlorella vulgaris, with an algae cell density of > 106 cells/mL.
- Feeding frequency: 5x weekly

Study design

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

21 d

Test conditions

Test temperature:

20±2 °C

pH:

7.5 - 8.2

Dissolved oxygen:

8.0 - 9.59 mg/L

Nominal and measured concentrations:

Nominal concetrations: 0.013, 0.032, 0.08, 0.2, 0.5 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Material, size, fill volume: Glass beakers (5 cm ID x 8 cm H), 100 mL; Volume of the study medium is 50 mL
- No. of organisms per vessel: 1
- No. of vessels per concentration (replicates): 10
- No. of vessels per control (replicates): 10
- Renewal rate of test solution: 3 times per week ( monday, wednesday and friday)



TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: A natural river water of agricultural backround, middle reach of the river "Böhme", Lower-Saxony was used.
Test water parameter:
pH: 8.2
Conductivity [μS/cm]: 397
DOC [mg C/L]: 7.3
TOC [mg C/L]: 9.9
Ammonium-N [mg N/L]: 0.141
Nitrate-N [mg N/L]: 2.38
o-Phosphate-P [mg P/L]: 0.031
Total Phosphate [mg P/L]: 0.128
Suspended Matter [mg/L]: 17.4
Total Hardness [mg CO3/L]: 91.3
- Culture medium different from test medium: yes


OTHER TEST CONDITIONS
- Photoperiod: 16/8 hours light dark cycle
- Light intensity: Diffuse light, illumination range max. 20 μE⋅m-2 ⋅ s-1


EFFECT PARAMETERS MEASURED: reproduction and mortality

Reference substance (positive control):

yes

Remarks:

potassiumdichromate

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

21 d

Dose descriptor:

NOEC

Effect conc.:

0.013 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

reproduction

Effect concentrations 2

Duration:

21 d

Dose descriptor:

LOEC

Effect conc.:

0.032 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

reproduction

Effect concentrations 3

Duration:

21 d

Dose descriptor:

EC50

Effect conc.:

0.34 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

reproduction

Results with reference substance (positive control):

- Results with reference substance valid? yes
- Relevant effect levels: EC50 = 2.1 (24h)

Any other information on results incl. tables

Table 1: Summary of all test item related effects in the test concentrations:

Effects	Nominal concentrations (mg/L)
	0.013	0.032	0.08	0.02	0.5
Reproduction rate	--	++	++	++	++
Stillborn juveniles	--	++	++	++	++
Aborded eggs	--	--	--	--	++
immobilization	--	--	--	--	--
Dry weight	--	--	--	--	++
Lenght	--	--	--	--	++
Males/winter eggs	--	--	--	--	--
Summary:	--	++	++	++	++

-- no significant effect

++ significant effect