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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Acute Toxicity: dermal

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Administrative data

acute toxicity: dermal
Type of information:
experimental study
Adequacy of study:
supporting study
Study period:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: see 'Remark'
Pre-guideline method, but data comprehensible and scientifically acceptable. (GLP: Yes) Bronaugh, R.L., R.F. Stewart and E. R. Congdon (1982) Methods for in vitro percutaneous absorption studies II. Animal Models for Human Skin Toxicology and Applied Pharmacology 62, 481-488
Reason / purpose for cross-reference:
reference to same study

Data source

Reference Type:
study report
Report date:

Materials and methods

GLP compliance:
yes (incl. QA statement)
Test type:
other: single 24 hours exposure skin penetration test

Test material

Constituent 1
Chemical structure
Reference substance name:
Methylphosphonic acid, compound with amidinourea (1:1)
EC Number:
EC Name:
Methylphosphonic acid, compound with amidinourea (1:1)
Cas Number:
Molecular formula:
Phosphonic acid, P-methyl-, compd. with N-(aminoiminomethyl)urea (1:1)
Test material form:
other: solution
Details on test material:
technical product

Test animals


Results and discussion

Applicant's summary and conclusion

Interpretation of results:
practically nontoxic
Migrated information
The test article FLOVAN CGN was assessed for its potential to permeate through porcine skin.
The assay was performed in two independent experiments over a time scale of 0 to 24 hours. The test article was tested at the following concentration (a.i):
Experiment I and II: 0.94 mg/ml in water. Samples were drawn from the receptor chambers following a fixed schedule:
Exp. I and II: 0.0; 0.5; 1.0; 2.0; 4.0, 6.0; 8.0 and 24 hours following the application of the test article.
The samples (0.5 ml each) were labelled and transferred to the sponsor's facilities for subsequent analysis.

Specimens of a pig ear skin penetration study were separated by reversed phase HPLC follwed by UV-detection at 200 nm. The separated and detected FLOVAN
CGN a.i. was quantified by an external calibration function. The bottom quantification limit of the practical work amounted to 1.28 µg/mL FLOVAN CGN a.i. in aqueous solution. By means of a serial connection of two analytical HPLC-columns penetrated FLOVAN CGN a.i. in the specimens of the 24 h sampling interval were quantified. Within 24 hours less than 5 % (mean value) of the amount of FLOVAN CGN applied to the test system penetrated*. Within 8 hours no MPAAU were detected in the receptor chamber.

*) test substance applied to the test system: 212.44 µg FLOVAN CGN a.i.; 452.00 µg FLOVAN CGN
5 % of the test substance: 10.62 ug FLOVAN CGN a.i.; 22.60 ug FLOVAN CGN

Discussion: Since permeation of the test article through porcine skin was very low a graphical evaluation of the analytical data was impossible. The concentration of the permeated test article did not exceed the lower limit of detection prior to 8 hours after start of experiments.

In conclusion, it can be stated that FLOVAN CGN showed very low penetration through the skin of porcine ears. Within 8 hours no MPAAU were detected in the receptor chamber. Within 24 hours less than 5 % (mean value) of the amount of FLOVAN CGN applied to the test system penetrated.