reaction products of diglycidyl ether bisphenol F (DGEBF) and oligomeric phenol diglycidyl ethers with acrylic acid

Endpoint summary

Additional information

For the assessment of aquatic toxicity of Epoxy half acrylate short-term toxicity data with fish, aquatic invertebrates, algae and microorganisms are available.

 

Short term toxicity to fish

In a 96 h acute toxicity study according to OECD guideline 203, adopted 17 July 1992, Zebrafish (Danio rerio) were exposed to Epoxy half acrylate

(100% a.i.) at nominal concentrations of 0, 0.625, 1.25, 2.50, 5.00 and 10.0 mg/L, corresponding to geometric mean weighted loading levels of 0, 0.239, 0.599, 1.25, 3.27 and 4.98 mg/L under semi-static conditions with daily renewal. 7 test organisms were exposed to each loading level and the control.

The concentrations of the analytes of the test item were analytically verified by LC-MS/MS from freshly prepared test media after 0 and 72 hours and corresponding 24 h aged test media after 24 and 96 hours of all concentration levels and the control.

Effect levels are given based on overall geometric mean weighted test item loadings.

100% mortality was observed at 4.98 mg/L. Sublethal effects (hyperventilation and missing escape reflex) were observed at a concentration of 3.27 mg/L. No mortality or sublethal effects were present at up to 1.25 mg/L.

The 96 h LC50 was 2.02 mg/L (95% CL: 1.25-3.27 mg/L). 

 

Short term toxicity to aquatic invertebtates

The 48 hr acute toxicity of Epoxy half acrylate to Daphnia magna was studied under static conditions. Daphnids were exposed to control and test chemical at nominal concentrations of 0 (control), 10, 18, 32, 56 and 100 mg/L, corresponding to measured concentrations of 0, 6.4, 5.92, 5.48, 6.94 and 4.57 mg/L. Due to the low water solubility the correlation between nominal and measured concentration was very poor. At the end of the test the measured values were higher than at the beginning of the test (except of the treatment 32 mg/L). This was probably caused by dissolved excrements of the daphnia as it can be seen in the control. Therefore, the nominal concentrations were used for the determination of the biological results.

Mortality/immobilisation and sublethal effects were observed daily. Two concentrations showed toxicity between 0 and 100% immobilisation. None of the animals were immobilised in the control.

The 24 h-EC50 of potassium dichromate was tested in a current positive control test. The value was determined as 1.1 mg/L, lying within the demanded range of 0.6 – 1.7 mg/L.

The 48 hour EC50 of Epoxy half acrylate was determined to be 55 mg/L (nominal).

 

Toxicity to aquatic algae and cyanobacteria

In a 72 hour acute toxicity study, the cultures of Desmodesmus subspicatus, strain CHODAT were exposed to Epoxy half acrylate at nominal concentrations of 0 (control), 1, 2.2, 4.6, 10, 22, 46, 100 mg/L under static conditions in accordance with OECD guideline 201. As the test item is poorly soluble in water, the water-accommodated fraction was tested. This was done by shaking the nominal load with the appropriate amount of deionised water for 24 hours, followed by membrane filtration.

At the beginning and at the end of the test, the content of test item was determined by DOC-determination in the test solutions. The content of the test item was calculated from the DOC measurement. Due to the low water solubility the correlation between nominal and measured concentration was very poor. Therefore, the nominal concentrations were used for the determination of the biological results.

The 72 h NOEC and 72 h EC50 based on growth rate were 4.6 and 315 mg/L. The 72 h NOEC and 72 h EC50 based on yield were 4.6 and 8 mg/L.

 

Toxicity to microorganisms

In a 3 h respiration inhibition study, activated sludge a predominantly municipal sewage treatment plant was exposed to Epoxy half acrylate at nominal concentrations of 0 (control), 10, 32, 100, 320 and 1000 mg/L in accordance with OECD Guideline 209 (2010). Copper (II) sulphate pentahydrate at concentrations of58, 100 and 180 mg/L was used as reference substance. The respiration rates of the control, reference and test item replicates were measured after a contact time of three hours, and the inhibitory effects of the test and reference item were determined in comparison to the control respiration rates. The mean inhibition of respiration for the test item replicates ranged from 3%to 63%.

The 3 h EC50 of Epoxy half acrylate was determined to be 594 mg/L, the 3 h EC10 was 31.7 mg/L, the 3 h NOEC was 10 mg/L.

 

The most sensitive organism to Epoxy half acrylate was Danio rerio. Thus, the lowest acute toxicity value is the 96 h LC50 to Danio rerio of 2.02 mg/L. This value is used for the calculation of PNECs.

Based on these results and the substance being not rapidly biodegradable, Epoxy half acrylate has to be classified as Aquatic Chronic 2.