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Ecotoxicological information

Long-term toxicity to fish

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Administrative data

Endpoint:
long-term toxicity to fish, other
Type of information:
experimental study
Remarks:
Study on constituent of UVCB substance.
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study with acceptable restrictions
Remarks:
This study is added to the substance dataset in function of the PBT assessment. It concerns one of the constituents of the UVCB substance.

Data source

Reference
Reference Type:
study report
Title:
Unnamed

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 204 (Fish, Prolonged Toxicity Test: 14-day Study)
Principles of method if other than guideline:
Fish were exposed 21 days, in contrast with the prescribed 14 days.

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- beige fine powder

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
yes
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Chemical name of vehicle: acetone and HCO-30

Test organisms

Test organisms (species):
Oryzias latipes
Details on test organisms:
TEST ORGANISM
- Common name: Oryzias latipes

Study design

Test type:
flow-through
Water media type:
freshwater
Limit test:
no
Total exposure duration:
21 d

Test conditions

Test temperature:
24 +/- 1°C
Nominal and measured concentrations:
Nominal concentrations: 0.0051, 0.0102, 0.0225, 0.045, 0.0941 and 0.2045 mg/L
Value was indicated based on arithmetic mean values of measured concentrations because some analysis results exceeded +/- 20 % of nominal conc
Details on test conditions:
TEST SYSTEM
- Test vessel: container
- Type of flow-through: continuous dilution with metering pump
- Renewal rate of test solution: 35L/day
- No. of organisms per level: 20

OTHER TEST CONDITIONS
- Photoperiod: 16h light / 8h dark

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2.1
- Test concentrations: 0.0051, 0.0102, 0.0225, 0.045, 0.0941 and 0.2045 mg/L

Results and discussion

Effect concentrationsopen allclose all
Duration:
7 d
Dose descriptor:
LC50
Effect conc.:
0.12 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
not specified
Remarks on result:
other: 95% confidence interval: 0.098-0.14 mg/L
Remarks:
Value was indicated based on arithmetic mean values of measured concentrations because some analysis results exceeded +/- 20 % of nominal conc
Duration:
14 d
Dose descriptor:
LC50
Effect conc.:
0.066 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
not specified
Remarks on result:
other: 95% confidence interval: 0.053-0.084 mg/L
Remarks:
Value was indicated based on arithmetic mean values of measured concentrations because some analysis results exceeded +/- 20 % of nominal conc
Duration:
21 d
Dose descriptor:
LC50
Effect conc.:
0.025 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
not specified
Remarks on result:
other: 95% confidence interval: 0.02 - 0.033 mg/L
Remarks:
Value was indicated based on arithmetic mean values of measured concentrations because some analysis results exceeded +/- 20 % of nominal conc
Duration:
21 d
Dose descriptor:
other: MLD
Effect conc.:
0.009 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
not specified
Remarks on result:
other: Value was indicated based on arithmetic mean values of measured concentrations because some analysis results exceeded +/- 20 % of nominal conc
Duration:
21 d
Dose descriptor:
LOEC
Effect conc.:
0.041 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
not specified
Remarks on result:
other: Value was indicated based on arithmetic mean values of measured concentrations because some analysis results exceeded +/- 20 % of nominal conc
Duration:
21 d
Dose descriptor:
NOEC
Effect conc.:
0.005 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
not specified
Remarks on result:
other: Value was indicated based on arithmetic mean values of measured concentrations because some analysis results exceeded +/- 20 % of nominal conc

Applicant's summary and conclusion

Validity criteria fulfilled:
not specified
Conclusions:
Long-term toxicity of o-terphenyl to fish. After 21 days exposure, the LC50 is 0.025 mg/L, the LOEC is 0.041 mg/L and the NOEC is 0.0048 mg/L, based on measured concentrations (arithmic mean).
Executive summary:

The long-term toxicity to Ozyzias latipes was assessed according to OECD 204, in a flow-through test set-up. Fish were exposed for 21 d to increasing concentrations of o-terphenyl (0.0051, 0.0102, 0.0225, 0.045, 0.0941 and 0.2045 mg/L). After 21 days, the LC50, LOEC and NOEC were determined to be 0.025, 0.041 and 0.0048 mg/L, based on measured concentrations (arithmic mean).