Terphenyl, hydrogenated

Long-term toxicity to fish

Administrative data

Endpoint:

long-term toxicity to fish, other

Type of information:

experimental study

Remarks:

Study on constituent of UVCB substance.

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Remarks:

This study is added to the substance dataset in function of the PBT assessment. It concerns one of the constituents of the UVCB substance.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 204 (Fish, Prolonged Toxicity Test: 14-day Study)

Principles of method if other than guideline:

Fish were exposed 21 days, in contrast with the prescribed 14 days.

Test material

Test material information

Constituent 1

Reference substance name:

o-terphenyl

EC Number:

201-517-6

EC Name:

o-terphenyl

Cas Number:

84-15-1

Molecular formula:

C18H14

IUPAC Name:

1,1':2',1''-terphenyl

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

yes

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Chemical name of vehicle: acetone and HCO-30

Test organisms

Test organisms (species):

Oryzias latipes

Details on test organisms:

TEST ORGANISM
- Common name: Oryzias latipes

Study design

Test type:

flow-through

Water media type:

freshwater

Limit test:

no

Total exposure duration:

21 d

Test conditions

Test temperature:

24 +/- 1°C

Nominal and measured concentrations:

Nominal concentrations: 0.0051, 0.0102, 0.0225, 0.045, 0.0941 and 0.2045 mg/L
Value was indicated based on arithmetic mean values of measured concentrations because some analysis results exceeded +/- 20 % of nominal conc

Details on test conditions:

TEST SYSTEM
- Test vessel: container
- Type of flow-through: continuous dilution with metering pump
- Renewal rate of test solution: 35L/day
- No. of organisms per level: 20

OTHER TEST CONDITIONS
- Photoperiod: 16h light / 8h dark

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2.1
- Test concentrations: 0.0051, 0.0102, 0.0225, 0.045, 0.0941 and 0.2045 mg/L

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

7 d

Dose descriptor:

LC50

Effect conc.:

0.12 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

not specified

Remarks on result:

other: 95% confidence interval: 0.098-0.14 mg/L

Remarks:

Value was indicated based on arithmetic mean values of measured concentrations because some analysis results exceeded +/- 20 % of nominal conc

Effect concentrations 2

Duration:

14 d

Dose descriptor:

LC50

Effect conc.:

0.066 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

not specified

Remarks on result:

other: 95% confidence interval: 0.053-0.084 mg/L

Remarks:

Value was indicated based on arithmetic mean values of measured concentrations because some analysis results exceeded +/- 20 % of nominal conc

Effect concentrations 3

Duration:

21 d

Dose descriptor:

LC50

Effect conc.:

0.025 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

not specified

Remarks on result:

other: 95% confidence interval: 0.02 - 0.033 mg/L

Remarks:

Value was indicated based on arithmetic mean values of measured concentrations because some analysis results exceeded +/- 20 % of nominal conc

Effect concentrations 4

Duration:

21 d

Dose descriptor:

other: MLD

Effect conc.:

0.009 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

not specified

Remarks on result:

other: Value was indicated based on arithmetic mean values of measured concentrations because some analysis results exceeded +/- 20 % of nominal conc

Effect concentrations 5

Duration:

21 d

Dose descriptor:

LOEC

Effect conc.:

0.041 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

not specified

Remarks on result:

other: Value was indicated based on arithmetic mean values of measured concentrations because some analysis results exceeded +/- 20 % of nominal conc

Effect concentrations 6

Duration:

21 d

Dose descriptor:

NOEC

Effect conc.:

0.005 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

not specified

Remarks on result:

other: Value was indicated based on arithmetic mean values of measured concentrations because some analysis results exceeded +/- 20 % of nominal conc

Applicant's summary and conclusion

Validity criteria fulfilled:

not specified

Conclusions:

Long-term toxicity of o-terphenyl to fish. After 21 days exposure, the LC50 is 0.025 mg/L, the LOEC is 0.041 mg/L and the NOEC is 0.0048 mg/L, based on measured concentrations (arithmic mean).

Executive summary:

The long-term toxicity to Ozyzias latipes was assessed according to OECD 204, in a flow-through test set-up. Fish were exposed for 21 d to increasing concentrations of o-terphenyl (0.0051, 0.0102, 0.0225, 0.045, 0.0941 and 0.2045 mg/L). After 21 days, the LC50, LOEC and NOEC were determined to be 0.025, 0.041 and 0.0048 mg/L, based on measured concentrations (arithmic mean).