Tin sulphate

Long-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

long-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

3 (not reliable)

Rationale for reliability incl. deficiencies:

other: see 'Remark'

Remarks:

no glp, no guideline a available (older than guideline and glp) water from lakes was used for experiment; In 1972 water from lakes contained high amounts of chelating agent (e.h. EDTA) Chelating agent stabilze Sn(II) and avoid the formation of hydroxide. So the amount of Sn(II) in water in the study measured is higher than the solubility of Sn(II) at this pH.(see discussion in endpoint summary Environmental fate and pathways)

Justification for type of information:

Justification for read across: stannous sulphate and stannous chloride are the salts from strong acids (here: hydrochloric and sulfuric acid) with Sn(II). In water there is a complete dissociation in the Sn(II) and the corrospondenting acid.
In the study water phase the concentration of chloirde and sulfate from natural sources is dimension granter than from the test item. So the chloirde and the sulfate is insignificant. For adverse effects only the Sn(II) is responsible

Data source

Reference

Reference Type:

publication

Title:

Unnamed

Year:

1972

Report date:

1972

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 211 (Daphnia magna Reproduction Test)

Deviations:

not applicable

Principles of method if other than guideline:

For chronic studies five daphnia 12 +/- 12 hr old were placed in 200 ml water in a 250 ml beaker. Four beakers (the test chambers) were used with total 20 animals for each experimental and control condition for each test. The test chambers were covered wirh a pane glase to minimize evaporation, and distributed randomly on a shelf in a constant temperature (18°C +/- 1) Young daphnia were placed in the test chambers, transferd into a new medium at the beginning of each week with a medicine dropper, an subjected to test conditions for 3 wks. Surival of the initial animals was noted after 1, 2 and 3 wks. At the end of the week 3 the surival animals was weighed and prepared for protein and GOT analyses. Reproduction was accessed by counting the young produced every week; the young was counted and discarded each time the inital animals were transfered and at the end of each experiment

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Tin dichloride

EC Number:

231-868-0

EC Name:

Tin dichloride

Cas Number:

7772-99-8

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Daphnia magna

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

3 wk

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

3 wk

Dose descriptor:

other: Chronik, 16% reproductive impairment

Effect conc.:

0.35 mg/L

Nominal / measured:

nominal

Conc. based on:

dissolved

Basis for effect:

reproduction

Remarks on result:

other: value for Sn2+

Effect concentrations 2

Duration:

21 d

Dose descriptor:

NOEC

Effect conc.:

0.18 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

dissolved

Remarks:

Sn

Basis for effect:

reproduction

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The value for 16% reproductive impairment was determinated to be 0.35 mg Sn^{+2} /L = 0.63 mg SnSO_4 / L

NOEC(21d)=0.18mg/L