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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Specific investigations: other studies

Currently viewing:

Administrative data

Endpoint:
endocrine system modulation
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
other: Scientific investigation not meeting standards as in guideline studies.

Data source

Referenceopen allclose all

Reference Type:
publication
Title:
Estrogenic Activities of 517 Chemicals by Yeast Two-Hybrid Assay.
Author:
Nishihara T, Nishikawa J, Kanayama T, Dakeyama F, Saito K, Imagawa M, Takatori S, Kitagawa Y, Hori S, Utsumi H
Year:
2000
Bibliographic source:
J. Health Sci., Vol. 46(4), 282-298, 2000
Reference Type:
publication
Title:
No information
Author:
Nishikawa J. et al.
Year:
1998
Bibliographic source:
Jpn. J. Toxicol. Environ. Health, Vol. 44, P-32, 1998; cited in Nishihara T. et al., J. Health Sci., Vol. 46(4), 282-298, 2000.

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
The aim of this study was to assess the estrogenic activities of environmentally relevant chemicals including melamine.
Method according to Nishikawa et al. The yeast two-hybrid system is based on the ligand-dependent interaction of two proteins, a hormone receptor and a coactivator, and hormonal activity is detected by ß-galactosidase activity.
GLP compliance:
not specified
Type of method:
in vitro

Test material

Constituent 1
Chemical structure
Reference substance name:
Melamine
EC Number:
203-615-4
EC Name:
Melamine
Cas Number:
108-78-1
Molecular formula:
C3H6N6
IUPAC Name:
1,3,5-triazine-2,4,6-triamine
Details on test material:
melamine

Test animals

Species:
other: Saccharomyces cerevisiae Y 190

Administration / exposure

Route of administration:
other: in vitro assay
Duration of treatment / exposure:
Exposure period: 4 hour(s)
Details on study design:
Yeast Two-Hybrid Assay.

Results and discussion

Any other information on results incl. tables

Melamine showed no estrogenic activity within the Yeast Two-Hybrid assay.

Applicant's summary and conclusion

Conclusions:
Melamine showed no estrogenic activity within the Yeast Two-Hybrid assay.
Executive summary:

Melamine showed no estrogenic activity within the Yeast Two-Hybrid assay.