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Environmental fate & pathways

Bioaccumulation: aquatic / sediment

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Reference
Endpoint:
bioaccumulation in aquatic species: fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Data is from authoritative database
Justification for type of information:
Data is from authoritative database
Qualifier:
according to
Guideline:
other: "Bioaccumulation test of a chemical substance in fish or shellfish" provided in "the Notice on the Test Method Concerning New Chemical Substances"
Principles of method if other than guideline:
BCF value of test chemical was determined in fish cyprinus carpio for 6 week on the basis of lipid content
GLP compliance:
not specified
Specific details on test material used for the study:
- Purity: 99 %, - Water solubility : 10 ppm
Radiolabelling:
not specified
Details on sampling:
2w, 3w, 4w, and 6w ( w= weeks)
Vehicle:
yes
Test organisms (species):
Cyprinus carpio
Route of exposure:
aqueous
Test type:
static
Details on test conditions:
RANGE-FINDING / PRELIMINARY STUDY - Test concentrations : Test concentrations : TLm(48h) 6.4 ppm(w/v), Rice fish (Oryzias latipes)
Concentration of vehicle: 1st Concentration area: 500 ppb(w/v), 2nd Concentration area: 50 ppb(w/v)
Nominal and measured concentrations:
Nominal concentrations 1st Concentration area: 100 ppb(w/v), 2nd Concentration area: 10 ppb(w/v)
Lipid content:
3 %
Remarks on result:
other: E. G . Bligh and W. J . Dyer, Can. J. Biochem, Physiol., 37, 911 (1959)
Conc. / dose:
0 mg/L
Type:
BCF
Value:
>= 16 - <= 21 L/kg
Basis:
total lipid content
Time of plateau:
6 wk
Remarks on result:
other: other details not available
Conc. / dose:
0 mg/L
Type:
BCF
Value:
>= 8.1 - <= 18 L/kg
Basis:
total lipid content
Time of plateau:
6 wk
Remarks on result:
other: other details not available

 

2w

3w

4w

6w

1st Concentration area

 

Test substance concentration (ppb (w/v))

97.6

96.3

95.9

97.2

Bioconcentration factor L/kg  

17

18

19

19

18

21

16

16

2nd Concentration area

 

Test substance concentration (ppb (w/v))

9.83

9.83

9.86

10.1

Bioconcentration factor L/kg  

(18)

(16)

(9.9)

(17)

 

(16)

(13)

(8.1)

(14)

 

Validity criteria fulfilled:
not specified
Conclusions:
The bioconcentration factor (BCF) for test chemical was determined according static fish test. The BCF value was observed to be 16-21 L/kg at dose concentration 0.0001 mg/L and 8.1-18 L/kg at dose concentration 0.00001 mg/L on test organism Cyprinus carpio during 6 weeks period. Therefore it is concluded that this test chemical is nonbioaccumulative
Executive summary:

Bioaccumulation test was conducted for 6 weeks for determination the bioconcentration factor (BCF) of test chemical on test organism Cyprinus carpio.

 

Recovery ratio : Test water : 1st concentration area : 90.1 %, 2nd concentration area : 79.6 %, Fish : 92.7 %, - Limit of detection : Fish : 0.

ppm

And Nominal concentrations used in the study as - 1st Concentration area: 0.0001 mg/L, 2nd Concentration area : 0.00001 mg/L and Range finding study was carried out on Rice fish (Oryzias latipes) TLm(48h) 6.4 ppm(w/v), Rice fish (Oryzias latipes).

 

Thus according to static Fish test the bioconcentration factor (BCF) for test chemical was determined according static fish test. The BCF value was observed to be 16-21 L/kg at dose concentration 0.0001 mg/L and 8.1-18 L/kg at dose concentration 0.00001 mg/L on test organism Cyprinus carpio during 6 weeks period.

 

Therefore it is concluded that this test chemical is nonbioaccumulative

Description of key information

The bioconcentration factor (BCF) for test chemical was determined according static fish test. The BCF value was observed to be 16-21 L/kg at dose concentration 0.0001 mg/L and 8.1-18 L/kg at dose concentration 0.00001 mg/L on test organism Cyprinus carpio during 6 weeks period. Therefore it is concluded that this test chemical is nonbioaccumulative

Key value for chemical safety assessment

BCF (aquatic species):
21 L/kg ww

Additional information

Various experimental studies and one prediction study have been conducted to determine bioaccumulation concentartion factor (BCF) of test chemical and their results are summarized below

In first study the Bioaccumulation test was conducted for 6 weeks for determination the bioconcentration factor (BCF) of test chemical on test organism Cyprinus carpio.

 Recovery ratio : Test water : 1st concentration area : 90.1 %, 2nd concentration area : 79.6 %, Fish : 92.7 %, - Limit of detection : Fish : 0.

ppm And Nominal concentrations used in the study as - 1st Concentration area: 0.0001 mg/L, 2nd Concentration area : 0.00001 mg/L and Range finding study was carried out on Rice fish (Oryzias latipes) TLm(48h) 6.4 ppm(w/v), Rice fish (Oryzias latipes).

Thus according to static Fish test the bioconcentration factor (BCF) for test chemical was determined according static fish test. The BCF value was observed to be 16-21 L/kg at dose concentration 0.0001 mg/L and 8.1-18 L/kg at dose concentration 0.00001 mg/L on test organism Cyprinus carpio during 6 weeks period.

Therefore it is concluded that this test chemical is nonbioaccumulative.

Next study was also reviewed from authoritative database in this the BCF value of test chemical was estimated is 57 dimensionless by using log Kow of 3.50 and regression derived equation and it is far less than 2000 so it is concluded that this chemical is non bioaccumulative.

Last study was the prediction done from EPI suite (2018) in this the estimated bioconcentration factor (BCF) for test chemical iwas estimated to be 57.07L/kg wet-wt. This value does not exceed the bioconcentration threshold of 2000. Thus it is concluded that test chemical is not expected to bioaccumulate in the food chain.

On the basis of all the studies mentioned above it is concluded that the BCF value of test chemical is in range of 8.1 to 57 dimensionless. By considering this range BCF value it is concluded that test chemical is non- bioaccumulative in aquatic species.