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Short-term toxicity to aquatic invertebrates

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Description of key information

The test item was assessed using Daphnia magna in a study according to EU Method C.2, OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test) and OPPTS 850.10.10. Young Daphnia were exposed to aqueous test media containing the test item for 48 hours. Based on results obtained in a preliminary experiment Daphnia were exposed to a single concentration of 100 mg/L (limit concentration). A concurrent control group was run. 
A static test was performed as the test item was previously shown to be stable in the test medium for 48 hours. The analytically measured test item concentration remained within ± 20 % of the nominal during the test period of 48 hours; therefore, biological results are based on the nominal concentration.
Healthy, young female daphnids with known origin, history and acclimatisation conditions were used. Twenty Daphnia (divided into 4 concurrent batches) were tested each, exposed to 100 mg/L test item and in the control. Each test vessel contained approximately 50 mL test medium. Fully characterized Elendt M4 Medium was used as test medium. The immobilisation of the test animals was recorded 24 and 48 hours after treatment. Environmental conditions were recorded. All measured values remained within acceptable ranges.
All validity criteria were met. There was no immobilisation observed in the twenty daphnids exposed to either the test item or in the control group. In addition to immobility, no abnormal behaviour or appearance of test animals was noted. The 48-h NOEC was determined to be 100 mg/L and the LOEC, EC100 and the EC50 values were determined to be > 100 mg/L.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
100 mg/L

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