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Environmental fate & pathways

Adsorption / desorption

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Administrative data

Endpoint:
adsorption / desorption: screening
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2010
Report Date:
2010

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to
Guideline:
OECD Guideline 121 (Estimation of the Adsorption Coefficient (Koc) on Soil and on Sewage Sludge using High Performance Liquid Chromatography (HPLC))
Version / remarks:
2001
Qualifier:
according to
Guideline:
EU Method C.19 (Estimation of the Adsorption Coefficient (KOC) on Soil and Sewage Sludge Using High Performance Liquid Chromatography (HPLC))
Version / remarks:
2008
GLP compliance:
yes
Type of method:
HPLC estimation method
Media:
soil/sewage sludge

Test material

Reference
Name:
Unnamed
Type:
Constituent
Radiolabelling:
no

Study design

Test temperature:
30 °C

HPLC method

Details on study design: HPLC method:
EQUIPMENT
- Apparatus: 515 HPLC Pump, 717 Plus Autosampler (Waters, Milford, MA, USA)
- Type, material and dimension of analytical (guard) column: Hypersil BDS-CN, 150 x 4.6 mm i.d., dp = 5 µm (Thermo Fisher Scientific, Waltham, MA, USA)
- Detection system: Photodiode Array Dectector 2996, UV dectection at 210 nm (Waters, Milford, MA, USA), Refractive Index Detector 2414, sensitivity = 4, time constant = 1 sec (Waters, Milford, MA, USA)


MOBILE PHASES
- Type: 55/45 (v/v) Methanol/Water
- Experiments with additives carried out on separate columns: no
- Solutes for dissolving test and reference substances: Methanol

REFERENCE SUBSTANCES
- Identity: Acetanilide

DETERMINATION OF RETENTION TIMES
- Quantity of test substance introduced in the column: 10 µl
- Quantity of reference substances: 10 µl

REPETITIONS
- Number of determinations: 2

Results and discussion

Adsorption coefficientopen allclose all
Type:
Koc
Value:
< 18
Type:
log Koc
Value:
< 1.26

Results: HPLC method

Details on results (HPLC method):
UV detection
- Retention times of reference substances used for calibration: Acetanilide: 2.23 min (Log Koc: 1.26)
- Retention time of test Substance: 1.91 min
RI detection
- Retention times of reference substances used for calibration: Acetanilide: 2.46 min (Log Koc: 1.26)
- Retention time of test Substance: 2.14 min

It was concluded that the log Koc of the test substance was <1.26 (Koc <18)

Applicant's summary and conclusion