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EC number: 468-710-7 | CAS number: 754-12-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2006
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- - concentrations - all
- Sampling method: 100 uL taken and added to appropriate vials to maintain conconcentation
- Sample storage conditions before analysis: stored at 2-10 cegrees Celcius and shipped to another facility at the end of the test (stability during storage and transport shown in another study) - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
Vial filled with test solutions that had been purged for 1 hour with the test material at a rate of approximately 10 mL/min. The test vessels were filled to the rim to minimize headspace and closed with transparent lids sealed with a silicone vacuum grease. This method was shown in another experiment to result in a saturated solution. - Test organisms (species):
- Cyprinus carpio
- Details on test organisms:
- TEST ORGANISM
- Common name: carp
- Source: Zodiac, LU Wageningen, the Netherlands
- Length at study initiation : 2.4-2.8 cm
- Weight at study initiation (mean and range, SD): 0.49 +/- 0.08g
- Method of breeding: Fi from a single parent-pair bred in UV treated water
- Feeding during test - no
ACCLIMATION
- Acclimation period: at least 12 days
- Acclimation conditions (same as test )
- Type and amount of food: pelleted fish food
- Feeding frequency: daily
- Health during acclimation (any mortality observed): no - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Test temperature:
- Day 0 - 21.1-21.2C
Day 4 - 21.5-21.3C - pH:
- Day 0 - 7.9 - 8.2
Day 4 - 7.3-7.2 - Dissolved oxygen:
- Day 0 - 6.4 - 9.2
Day 4 - 2.7 - 3.2 - Nominal and measured concentrations:
- nominal - above 100 mg/L
measured - 197 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: 10 liter, all glass closed with transparent lids sealed with silicone high vacuum grease
- Type : closed
- fill volume:9.8 liters
- Aeration:no
- No. of organisms per vessel:7
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 1
- Biomass loading rate: 0.15 g fish/liter
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 hours daily
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
TEST CONCENTRATIONS
- Spacing factor for test concentrations:Not applicable, limit test
- Test concentrations: 100 mg/L - Reference substance (positive control):
- yes
- Remarks:
- pentachlorophenol
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 197 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- - Behavioural abnormalities:
- Mortality of control: none
- Other adverse effects control:no - Validity criteria fulfilled:
- yes
- Remarks:
- no mortality in control, test conditions maintained during test, dissolved oxygen content at least 60% air saturation value, results based on geometric average measured concentrations
- Conclusions:
- The 96 hour LC50 for carp exposed to the test substance is > 197 mg/l and the test substance can be classified as not harmful to freshwater fish
Reference
Description of key information
HFO-1234yf has low toxicity to freshwater fish
Key value for chemical safety assessment
Additional information
HFO-1234yf has low toxicity to freshwater fish with a 96 hr LC50 greater than 197 mg/l.
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