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Ecotoxicological information

Long-term toxicity to fish

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Administrative data

Endpoint:
fish, juvenile growth test
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study with acceptable restrictions
Remarks:
The dissolved oxygen concentrations were not maintained at 70% or more of the air saturation value throughout the test, this was a consequence of testing a gas in air-tight closed vessels. However, no negative effects were observed.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2011
Report date:
2011

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to guideline
Guideline:
OECD Guideline 215 (Fish, Juvenile Growth Test)
Version / remarks:
2000
Qualifier:
according to guideline
Guideline:
EU Method C.14 (Fish Juvenile Growth Test)
Version / remarks:
2008
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
- Concentrations: All test concentrations and stock solution
- Sampling method: The samples were taken in a 20 ml GC-vial that already contained 0.5 ml acetone.
- Sample storage conditions before analysis: none

Test solutions

Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Preparation of stock solutions by loading two 10 L gas sampling bags each with 7 or 7.5 litres of ISO medium followed by addition of ~3.5 or 3.75 grams gas (500 mg/l). The gas sampling bags were closed immediately after addition and then shaken carefully for 4 hours on a platform shaker. Subsequently, the bags were left to settle overnight. The two saturated media (expected concentration ~160 mg/l) were mixed and then used for preparing the test concentrations by serial dilution.

Test organisms

Test organisms (species):
Cyprinus carpio
Details on test organisms:
TEST ORGANISM
- Common name: Carp
- Source: Zodiac, proefacc, "De Haar Vissen", Wageningen University and Research Centre, The Netherlands.
- Weight at study initiation (mean): 0.76 ± 0.08 g

FEEDING DURING TEST
- Food type: Cyprico Crumble Excellent (300-500 um), Coppens International bv, Helmond, The Netherlands).
- Amount: 4% of body weight per vessel
- Frequency: daily

Study design

Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
28 d

Test conditions

Hardness:
211.5 mg/L
Test temperature:
20.0 - 21.6 °C
pH:
6.9 - 7.9
Dissolved oxygen:
In freshly prepared solutions: 8.3 - 10.1 mg/L
In 24 h old solutions: 2.1 - 6.2 mg/L
In 24 h old control: 1.8 - 6.2 mg/L
Nominal and measured concentrations:
Nominal test substance concentrations: 1.5, 3.0, 6.0, 12 and 24 mg/L
Mean measured substance concentrations: 1.2, 2.7, 5.4, 11 and 22 mg/L (82 - 91% of nominal)
Details on test conditions:
TEST SYSTEM
- Test vessel: airthight glass vessels (40 L)
- Type: closed
- Fill volume: 38 L
- Aeration: none
- Renewal rate of test solution (frequency): daily
- No. of organisms per vessel: 16
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 0.32 g fish/L

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Adjusted ISO medium, formulated using RO-water (tapwater purified by reverse osmosis; GEON Waterbehandeling, Berkel-Enschot, The Netherlands)

OTHER TEST CONDITIONS
- Photoperiod: 16:8 h day-night regime

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Daily determination of mortalities, clinical sings were determined three times a week, body weight was determined at test start and after 14 and 28 days of exposure respectively.

Reference substance (positive control):
yes
Remarks:
penta-chlorophenol

Results and discussion

Effect concentrationsopen allclose all
Duration:
28 d
Dose descriptor:
EC50
Effect conc.:
> 22 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
growth rate
Duration:
28 d
Dose descriptor:
NOEC
Effect conc.:
2.7 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
growth rate
Duration:
28 d
Dose descriptor:
LOEC
Effect conc.:
5.4 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
growth rate
Details on results:
- Type of and number with morphological abnormalities: none
- Type of and number with behavioural abnormalities: none
- Other biological observations: no mortalities observed
Statistical analysis on the growth rates for the interval of days 0-14 showed that the growth rate of the fish exposed to test groups of 5.4 and 22 mg/l were statistically significant lower than the growth of the fish in the control group.
Results with reference substance (positive control):
The reference test showed normal sensitivity of the supplied carp.
Reported statistics and error estimates:
ANOVA, Tukey test, Bonferroni t-test, TOXSTAT Release 3.5, 1996, D.D. Gulley, A.M. Boelter, H.L. Bergman

Any other information on results incl. tables

Table 1: Mean fish weights recorded at the start, after 14 and 28 days.

Day of recording

Mean fish weights (g):

Control

1.2 mg/L

2.7 mg/L

5.4 mg/L

11 mg/L

22 mg/L

0

0.76

0.79

0.78

0.77

0.78

0.73

14

1.11

1.11

1.10

1.06

1.10

1.01

28

1.49

1.48

1.49

1.40

1.45

1.27

% increase on day 28*

196

189

191

182

186

174

*Based on non-rounded figures.

Table 2: Mean growth rates recorded for the intervals of 0-14 days and 0-28 days.

Interval

Mean growth rates:

Control

1.2 mg/L

2.7 mg/L

5.4 mg/L

11 mg/L

22 mg/L

Day 0-14

2.71

2.45

2.48

2.22

2.47

2.32

Day 0-28

2.42

2.28

2.32

2.11

2.20

1.96

 

Applicant's summary and conclusion