Guanidinium nitrate

Long-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

long-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

02 - 11/1984

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

test procedure in accordance with generally accepted scientific standards and described in sufficient detail

Reason / purpose for cross-reference:

reference to other study

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 211 (Daphnia magna Reproduction Test)

Principles of method if other than guideline:

no guideline mentioned but the method was similar as described in the guideline OECD Guideline 211, adopted 3 october 2008

GLP compliance:

not specified

Analytical monitoring:

yes

Vehicle:

no

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna
- Source: in-house culture unit
- Age of parental stock (mean and range, SD): neonates obtained from 11 day-old daphnids for the first daphnid chronic test, 32 day-old daphnids for the second daphnid chronic test
- Feeding during test
- Food type: Ankistrodesmus falcatus raised and supplemented with vitamins as recommended by Goulden et al., 1982
- Amount:2mg/L in the morning, 4mg/L in the afternoon
- Frequency: twice a day


ACCLIMATION
- Acclimation period:test started with less than 24hr old daphnids

Test type:

flow-through

Water media type:

freshwater

Limit test:

no

Total exposure duration:

21 d

Hardness:

1.test: 169mg/L CaCO3
2. test: 165-181mg/L CaCO3

Test temperature:

1. test: 18.3-21.1
2.test: 19.4-20.6

pH:

1.test: 7.8-8.3
2.test:7.8-8.2

Dissolved oxygen:

1.test: 8.1-8.5mg/L
2.test: 7.6-8.7mg/L

Nominal and measured concentrations:

1.test: nominal concentrations: 60/33.6/18.8/10.5/5.9/0mg/L
measured concentration: 52/26.2/16.8/8.8/4.2/<1mg/L
2.test: nominal concentrations: 6.19/3.45/1.97/1.08/0.63/0mg/L
measured concentrations: 6.09/2.9/1.66/1.14/0.75/<1mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Type: flow through
- Material, size, headspace, fill volume: 50ml beakers/25ml
- Type of flow-through: peristaltic
- Renewal rate of test solution (frequency/flow rate):two tank volumes of test solution per day
- No. of organisms per vessel:10
- No. of vessels per concentration (replicates):4
- No. of vessels per control (replicates):4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water:from 62m well at Ft. Detrick, water softening system, afterwards pumped through a spray nozzle for aeration and sent through a 5micron cellulose acetate cartidge filter, an UV sterilizer, and temperature control equipment prior to use in test
- Conductivity:868micro mhos/cm

OTHER TEST CONDITIONS
- Photoperiod:16hr light, 8hr dark
- Light intensity:400lux(test), 200lux(stock)

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
survival, total young per replicate tank, young per female per replicate day, growth reproduction rate, immobilization

Reference substance (positive control):

not specified

Key result

Duration:

21 d

Dose descriptor:

NOEC

Effect conc.:

2.9 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

reproduction

Remarks on result:

other: second test

Key result

Duration:

21 d

Dose descriptor:

LOEC

Effect conc.:

6.09 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

reproduction

Remarks on result:

other: second test

Duration:

21 d

Dose descriptor:

LOEC

Effect conc.:

4.2 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

reproduction

Remarks on result:

other: first test

Measured GN concentrations – first Daphnid chronic test:

nominal concentration (mg/L)	mean measured concentration (mg/L)	standard deviation	Range	n
60	52	-	-	1
33.6	26.2	±0.87	25-28	4
18.8	16.8	±2.87	15-21	4
10.5	8.8	±0.5	8-9	4
5.9	4.2	±2.06	2-7	4
0	<1^A		-	4

^Abelow detection limit

 

 

Results of first Daphnid chronic test with GN:

Mean measured concentration (mg/L)	Immobilization^A (%)	Young per Female per reproductive day^B	total young per tank^B	Length^C (mm)
52.0	100^D	-	-	-
26.2	22.5	1.02^D	83.8^D	2.89^D
16.8	5.0	2.91^D	331.5^D	3.4^D
8.8	7.5	4.24^D	468.8^D	3.78^D
4.2	12.5	6.22^D	683.5^D	4.18^D
<1(control)	12.5	7.93	914.0	4.51

^ATotal for four replicate tanks at each treatment level

^BMean of four replicate tanks at each treatment level

^CMean length per daphnid for four replicate tanks at each treatment level

^DSignificantly different from the controls (overalla=0.05)

 

Measured GN concentrations – second Daphnid chronic test:

nominal concentration (mg/L)	mean measured concentration (mg/L)	standard deviation	Range	n
6.19	6.09	±0.179	5.94-6.35	4
3.45	2.90	±0.143	2.77-3.05	4
1.97	1.66	±0.140	1.55-1.84	4
1.08	1.14	±0.162	1.04-1.38	4
0.63	0.75^A	±0.0520	0.72-0.81	3
0	<1^B	-	-	4

^Aestimated concentration

^BBelow detection limit

 

Results of second Daphnid chronic test with GN:

Mean measured concentration (mg/L)	Immobilization^A (%)	Young per Female per reproductive day^B	total young per tank^B	Length^C (mm)
6.09	0	9.32^D	1.305	5.01
2.90	0	10.2	1.432	5.12
1.66	0	10.7	1.499	5.17
1.14	5.0	10.5	1.397	5.23
0.75	7.5	10.6	1.400	5.24^D
<1(control)	0	10.6	1.453	5.11

^ATotal for four replicate tanks at each treatment level

^BMean of four replicate tanks at each treatment level

^CMean length per daphnid for four replicate tanks at each treatment level

^DSignificantly different from the controls (overalla=0.05)

Validity criteria fulfilled:

yes

Conclusions:

The 21-day NOEC based on reproduction was 2.90 mg/L.  Production of offsprings in the treated groups indicated that Guanidine Nitrate had an effect on the reproduction at concentrations greater than 6.09 mg/L. In a first test the toxicity seems to be higher with 4.2mg/L Guanidine Nitrate reported as the lowest concentration where a toxic effect was observed.

Executive summary:

The 21-day-chronic toxicity of Guanidine Nitrate to Daphnia magna was studied under flow through conditions. Daphnids were exposed to control and test chemical at nominal (measured) concentrations of 60, 33.6, 18.8, 10.5, and 5.9mg/L (52, 26.2, 16.8, 8.8, 4.2mg/L) in the first test and 6.19, 3.45, 1.97, 1.08, 0.63 mg/L (6.09, 2.90, 1.66, 1.14, 0.75mg/L) in the second test respectively. In the first test significant toxic effects were found at the lowest concentration tested, 4.2mg/L. Therefore a second test was performed with lower concentrations of Guanidine Nitrate.

 

The 21-day NOEC based on reproduction was 2.90 mg/L.  Production of offsprings in the treated groups indicated that Guanidine Nitrate had an effect on the reproduction at concentrations greater than 6.09 mg/L. In a first test the toxicity seems to be higher with 4.2mg/L Guanidine Nitrate reported as the lowest concentration where a toxic effect was observed.

This study is classified as acceptable and satisfies the guideline requirements for a chronic toxicity study with freshwater invertebrates.

 

Results Synopsis

 

Test Organism Age (e.g. 1^stinstar): 24hours

Test Type: Flowthrough

 

21-d-NOEL:  2.90mg/L

21-d-LOEC:  6.09mg/L / 4.2mg/L                    

Endpoint(s) Effected:  long-term toxicity to aquatic invertebrates, also ecotoxicological endpoints:

reproduction, immobilization

 

Description of key information

The long-term toxicity of Guanidine Nitrate to Daphnia magna was tested in a 21-day flow-through test.

The lowest concentration with an effect on reproduction (LOEC) was monitored at a concentration 4.2mg/L in a

first chronic test of van der Schalie, 1985 and 6.09mg/L in a second chronic test. The highest concentration of the

test item without an effect (NOEC) was found at 2.90mg/L.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Dose descriptor:

NOEC

Effect concentration:

2.9 mg/L

Additional information

The toxic effect of Guanidine Nitrate was monitored in a 21-day toxicity test. 2.9mg/L of the test substance was the highest concentration where no toxic effect was monitored. Because in a first chronic toxicity test of van der Schalie, 1985 an toxic effect was monitored at the lowest concentration tested (4.2mg/L), a second test was performed with lower concentrations tested. In this second test the lowest effect concentration was reported at 6.09mg/L. In another study of Cooney et al. (1985) the LOEC was higher. Therefore the van der Schalie study can serve as the key study. It is interesting to mention that Guaninine Nitrate is 10 to 20 times more toxic in a chronic 21-day toxicity test compared to an acute 48h toxicity test which is reported in the same study of van der Schalie, 1985.