Ethyl chloroacetate

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From 21.02.1979 to 29.05.1979

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: No data on guidelines but the test method is similar to OECD 401.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1979

Report date:

1979

Materials and methods

Principles of method if other than guideline:

The examination of acute oral toxicity was performed on female Wistar rats with an observation period of 14 days

GLP compliance:

no

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Ethyl chloroacetate

EC Number:

203-294-0

EC Name:

Ethyl chloroacetate

Cas Number:

105-39-5

Molecular formula:

C4H7ClO2

IUPAC Name:

ethyl 2-chloroacetate

Details on test material:

- Name of test material (as cited in study report): Monoehloressigsaureathylester
- Physical state: colorless liquid

Test animals

Species:

rat

Strain:

Wistar

Sex:

female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Weight at study initiation: 160-182 g (172 g ± 6,08 g; n=40)
- Fasting period before study: 16 hours
- Housing: Housing of the animals was carried o ut in groups in plastic cages with wood shavings.
- Diet (e.g. ad libitum): No feeding during 2 first hours post administration. During the test period of 14 days after administration, animals were feed ad libitum. Maintenance diet: Altromin 1324 (Altromin GmbH, Lage/Lipe).
- Water (e.g. ad libitum): tap water, ad libitum
- Acclimation period: No data

ENVIRONMENTAL CONDITIONS: No data

IN-LIFE DATES: From: 21.02.1979 To: 14.03.1979

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

other: sesame oil

Details on oral exposure:

VEHICLE
- Concentration in vehicle: 5g/100 mL

Doses:

80, 125, 200 and 315 mg/kg bodyweight

No. of animals per sex per dose:

10 female rats per dose (The study was performed on female rats because preliminary experiments demonstrated that they were more sensitive to toxicity)

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: Weekly
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs of poisoning and body weight. Fatally poisoned animals were dissected and the macroscopic autopsy findings were recorded.

Statistics:

The LD 50 was calculated using Probit (method according to Linder and Weber). The confidence limits were calculated according to CAVALLI-SFORZA(programmed by Department of Applied Mathematics of Hoechst Aktiengesellschaft).

Results and discussion

Effect levels

Sex:

female

Dose descriptor:

LD50

Effect level:

180 mg/kg bw

95% CL:

150.57 - 215.27

Mortality:

Mortality rate and death time of each animal were recorded.
Fatally poisoned animals died between 136 minutes and 24 hours post-administration with following clinical signs: squatting position, ruffled fur, disturbance of balance, abdominal position and passivity.

Clinical signs:

other: Surviving animals showed same symptoms in an attenuated form, and were free of symptoms 48 hours post administration. Animal behavior was normal throughout the study period.

Gross pathology:

The autopsy of all animals (dead during the study and euthanased) showed no signs of macroscopic alterations.

Any other information on results incl. tables

Table 07.02.02_01. Mortality

Dose	Concentration	Number of death animals /
(mg/kg)	(%)	Number of used animals
80	5	0/10
125	5	2/10
200	5	5/10
315	5	10/10

Applicant's summary and conclusion

Interpretation of results:

Toxicity Category III

Remarks:

Migrated information Criteria used for interpretation of results: EU

Conclusions:

The oral media lethal dose (LD 50) in female rats is 180 mg/kg body weight. The confidence interval for p=0.05 is 151 - 215 mg/kg body weight.

Executive summary:

The examination of acute oral toxicity was performed on female Wistar rats by oral gavage at the doses 80, 125, 200 and 315 mg/kg bodyweight. At the end of an observation period of 14 days a LD 50 of 180 (151 - 215) mg/kg body weight was determined by Probit.