2-[(2-[2-(dimethylamino)ethoxy]ethyl)methylamino]ethanol

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1990-06-18 to 1990-06-22

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

Version / remarks:

EEC Directive 67/548 Annex V C.1 as published in 84/449/EEC.

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

- Name of test material (as cited in study report): Texacat ZF-10, 2-[[2-[2-(dimethylamino)ethoxy]ethyl]emethylamino]ethanol
- Physical state: Clear colorless liquid
- Analytical purity: 97%
- Lot/batch No.: 90/329
- Expiration date of the lot/batch: Approximately two years from receipt (received on 30 March 1990)
- Storage condition of test material: dark at room temperature

Analytical monitoring:

yes

Details on sampling:

- Concentrations: Test concentrations were verified by chemical analysis.
- Sampling method: 100 m/L samples were taken from each vessel at 0, 24 and 96 hours.
- Sample storage conditions before analysis: N/A

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Direct dispersion in water.

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

TEST ORGANISM
- Common name: Rainbow trout
- Source: Westacre Trout Farm, Norfolk, U.K.
- Age at study initiation (mean and range, SD): N/A
- Length at study initiation (length definition, mean, range and SD): 6.0 cm (SD = 0.4 cm)
- Weight at study initiation (mean and range, SD): 4.55 g (SD = 0.52 gg)
- Feeding during test: None during test

ACCLIMATISATION
- Acclimation period: Stock held since 29 March 1990 and acclimatised to test conditions from 6 June 1990.
- Type and amount of food: Commercial trout pellets
- Feeding frequency: Daily, discontinued 24 h prior to test

Mortalities: less than 1% in 7 days prior to testing.

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Hardness:

CA 350 mg CaCO3/L

Test temperature:

14 ± 1 °C

Dissolved oxygen:

> 10 mg/L

Nominal and measured concentrations:

32, 56, 100, 180, and 320 mg/L (nominal)

Details on test conditions:

TEST SYSTEM
- Test vessel: Glass aquaria holding 40 L of test media.
- Material, size, headspace, fill volume: 40 L
- Aeration: Via narrow bore glass tubes.
- Renewal rate of test solution (frequency/flow rate): Daily
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Laboratory tap water, dechlorinated by the addition of sodium thiosulphate.

OTHER TEST CONDITIONS
- Photoperiod: 16 hours light : 8 hours dark

Reference substance (positive control):

no

Duration:

24 h

Dose descriptor:

LC50

Effect conc.:

> 320 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

> 320 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

72 h

Dose descriptor:

LC50

Effect conc.:

> 320 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 320 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

>= 320 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Details on results:

The only marked reaction to exposure was an increase in pigmentation observed in all fish at the highest test concentration of 320 mg/L.

Validity criteria fulfilled:

yes

Conclusions:

The LC50 was determined to be greater than 320 mg/L and the NOEC was greater than or equal to 320mg/L for all time points.

Description of key information

The Acute Toxicity study of test substance to rainbow Trout (Salmo Gairdneri) was conducted according to OEDC guideline 203 and follows GLP. The substance was found to be not harmful to fish.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

320 mg/L

Additional information

The acute toxicity of the test substance to rainbow Trout (Salmo Gairdneri, freshwater fish) for a duration of 96h was assessed according to the OECD guideline 203 and GLP requirements (Hunstman, 1990). Fish were exposed under semi-static conditions to 32, 56, 100, 180, and 320 mg/L (nominal concentrations) of substance dissolved in dilution water. Based on the test results the 96h LC50 was determined to be higher than 320 mg test item/L based on nominal concentrations and the NOEC was greater or equal to 320 mg/L at all time points.