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Long-term toxicity to aquatic invertebrates

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Reference
Endpoint:
long-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2009-01-07 to 2009-01-28
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 211 (Daphnia magna Reproduction Test)
Deviations:
no
GLP compliance:
yes (incl. QA statement)
Specific details on test material used for the study:
- Name of test material (as cited in study report): Jeffcat ZF-10, 2-[{2-[2-(dimethylamino)ethoxy]ethyl}methylamino]ethanol
- Physical state: clear liquid
- Lot/batch No.: DR30771206
Analytical monitoring:
yes
Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Stock solution of 56 mg/L was made by adding 0.112 g test substance to 2 L dilution water, and shaking vigorously. Appropriate ammounts were diluted to exposure concentrations by direct dilution of concentrate to 1000 ml of dilution water in volumetric flasks.
Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: Water flea
- Source: In-house culture
- Age of parental stock (mean and range, SD): 28 +/- 1 day
- Feeding during test
- Food type: Chlorella vulgaris and Pseudokirchneriella subcapitata
- Amount: 0.12 mg C/daphnid/L/day
- Frequency: Daily


Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
21 d
Post exposure observation period:
No
Hardness:
249-256 mg/L as CaCO3
Test temperature:
19.6-20.0 °C
pH:
7.8-8.3 SU
Dissolved oxygen:
7.8 - 9.0 mg/L
Salinity:
N/A
Nominal and measured concentrations:
Nominal- Control, 0.18, 0.56, 1.8, 5.6, 18, and 56 mg/L
Measured-Control, 0.20, 0.51, 1.9, 5.3, 19, and 54 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): covered
- Material, size, headspace, fill volume: 100 ml glass beakers, 80 ml test solution
- Aeration: no
- No. of organisms per vessel: 1
- No. of vessels per concentration (replicates): 10
- No. of vessels per control (replicates): 10


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Elendt's M4 Daphnia medium
- Total organic carbon:
- Particulate matter: <3 TSS
- Metals: Bo- 483, Co-2.3, Cu-6.4, Fe-156, Mn-95, Zn- 6.9
- Pesticides: BDL
- Chlorine: N/A
- Alkalinity: 34 mg/L CaCO3
- Ca/mg ratio: N/A
- Conductivity: 599 uS
- Salinity:N/A
- Culture medium different from test medium: No
- Intervals of water quality measurement: 3 times weekly, at water change


OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: 16 light/8 dark
- Light intensity: 6.6 u Einsteins/ m2/s


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Survival and reproduction, daily observation and counting


VEHICLE CONTROL PERFORMED: no


Reference substance (positive control):
no
Key result
Duration:
28 d
Dose descriptor:
NOEC
Effect conc.:
19 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
28 d
Dose descriptor:
LOEC
Effect conc.:
54 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
mortality
Key result
Duration:
28 d
Dose descriptor:
NOEC
Effect conc.:
1.9 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
reproduction
Duration:
28 d
Dose descriptor:
LOEC
Effect conc.:
5.3 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
reproduction
Duration:
28 d
Dose descriptor:
NOEC
Effect conc.:
1.9 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
growth
Remarks:
length
Duration:
28 d
Dose descriptor:
LOEC
Effect conc.:
5.3 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
growth
Remarks:
length
Key result
Duration:
28 d
Dose descriptor:
NOEC
Effect conc.:
1.9 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
other: overall
Details on results:
- Mortality of parent animals: No mortality through 18 mg/L, 40% in 56 mg/L on day 7, 100 by day 14
- No. of offspring produced per female: 48-84, concentration dependent
- 9 days to first brood
Reported statistics and error estimates:
Data were analysed using Minitab software. Data were analysed for normality and homogeneity of variance before appropriate procedures were applied to test for significant differencesbetween control and exposure concentrations.
Validity criteria fulfilled:
yes
Conclusions:
The overall No Observed Effect Concentration (NOEC), of the test substance was 1.9 mg/L.

Description of key information

Chronic Toxicity study of test substance to Daphnia magna was conducted  according to OECD guideline 211 and followed GLP. The NOEC was determined to be 1.9 mg/L.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
1.9 mg/L

Additional information

The chronic toxicity study of test substance to Daphnia magna was conducted according to OECD guideline 211 and followed GLP (Huntsman 2009). The overall NOEC value of test substance (28 days) was 1.9 mg/L.