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EC number: 211-162-9 | CAS number: 631-61-8
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- read-across based on grouping of substances (category approach)
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Justification for type of information:
- REPORTING FORMAT FOR THE CATEGORY APPROACH
See attached reporting format. - Reason / purpose for cross-reference:
- reference to other study
- Principles of method if other than guideline:
- Read-across approach from Letter of Access experimental data (EU Method C.4-A (Determination of the "Ready" Biodegradability - Dissolved Organic Carbon (DOC) Die-Away Test) on the analogue Sodium acetate.
- GLP compliance:
- yes
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material:
PHYSICO-CHEMICAL PROPERTIES
- Melting point: 324 ºC
- Boiling point: Decomposes above 400°C
- Vapour pressure: 0.00000000537 mm Hg at 25 ºC
- Henry's law constant (for volatie substances): 5.5 E -07 atm.m3/mole at 25 ºC
- Water solubility (under test conditions): 1.25 g/mL at 25 ºC
- Solubility in organic solvents: In ethanol: 0.053 g/cm3 at 25 ºC
- log Pow: -3.72
- pKa: At 20 ºC, it ranged from 1.26 to 1.45. At 25 ºC, it ranged from 0.18 to 0.20.
OTHER PROPERTIES (if relevant for this endpoint)
- Toxicity to microorganisms: (16 h) EC 50 = 7.2 g/L (growth inhibition) for Pseudomonas putida. - Inoculum or test system:
- activated sludge, domestic, non-adapted
- Key result
- Parameter:
- % degradation (DOC removal)
- Value:
- 99
- Sampling time:
- 28 d
- Key result
- Parameter:
- % degradation (DOC removal)
- Value:
- 99
- Sampling time:
- 27 d
- Key result
- Parameter:
- % degradation (DOC removal)
- Value:
- 91
- Sampling time:
- 21 d
- Key result
- Parameter:
- % degradation (DOC removal)
- Value:
- 93
- Sampling time:
- 14 d
- Key result
- Parameter:
- % degradation (DOC removal)
- Value:
- 86
- Sampling time:
- 7 d
- Details on results:
- Based on the experimental results obtained with the analogue Sodium acetate, which is considered to be readily biodegradable (> 99% in 28 days), and applying the read-across approach, the substance Ammonium acetate is also considered to be readily biodegradable under test conditions.
The analogue Sodium acetate which shares the same functional group with Ammonium acetate, also has comparable values for the relevant molecular properties. These properties are:
- vapour pressure value, which is 5.37 E-09 mmHg for Sodium acetate and 0.02 Pa for Ammonium acetate at 25 ºC,
- a high water solubility which is 1.25 g/mL at 25 ºC for Sodium acetate and 1480 g/L at 4 ºC for Ammonium acetate, and
- similar molecular weights which are 82.0 for Sodium acetate and 77.08 for Ammonium acetate. - Validity criteria fulfilled:
- not applicable
- Interpretation of results:
- readily biodegradable
- Conclusions:
- The substance Ammonium acetate is considered to be readily biodegradable under test conditions.
- Executive summary:
Based on the experimental results obtained with the analogue Sodium acetate (reported under the endpoint 05.02.01_02 NaAc), which is considered to be readily biodegradable (> 99% in 28 days), and applying the read-across approach, the substance Ammonium acetate is also considered to be readily biodegradable under test conditions.
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-A (Determination of the "Ready" Biodegradability - Dissolved Organic Carbon (DOC) Die-Away Test)
- Deviations:
- no
- GLP compliance:
- yes
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): waste water treatment plant: Germany
- Duration of test (contact time):
- 28 d
- Initial conc.:
- 10.2 mg/L
- Based on:
- DOC
- Parameter followed for biodegradation estimation:
- DOC removal
- Details on study design:
- TEST CONDITIONS
- Composition of medium: as described in appendix 3 in the study report.
- Test temperature: 21.5-22.6 ºC
TEST SYSTEM
- Culturing apparatus: 2000 ml Erlenmeyer
- Number of culture flasks/concentration: two flasks per concentration
- Measuring equipment: DOC analyser: infrared analyser T 500 (Shimadzu)
SAMPLING
- Sampling frequency: 0, 7, 14, 21, 27 and 28 days
CONTROL AND BLANK SYSTEM
- Inoculum blank: one replicate - Reference substance:
- benzoic acid, sodium salt
- Key result
- Parameter:
- % degradation (DOC removal)
- Value:
- 86
- Sampling time:
- 7 d
- Key result
- Parameter:
- % degradation (DOC removal)
- Value:
- 93
- Sampling time:
- 14 d
- Key result
- Parameter:
- % degradation (DOC removal)
- Value:
- 91
- Sampling time:
- 21 d
- Key result
- Parameter:
- % degradation (DOC removal)
- Value:
- 99
- Sampling time:
- 27 d
- Key result
- Parameter:
- % degradation (DOC removal)
- Value:
- 99
- Sampling time:
- 28 d
- Results with reference substance:
- 98% degradation at 7 days and 101% degradation at 28 days.
- Validity criteria fulfilled:
- yes
- Remarks:
- (the difference of extremes of replicate values of the removal of test chemical is less than 20% and the percentage degradation of the reference substance has reached the level for ready biodegradability by 14 days)
- Interpretation of results:
- readily biodegradable
- Conclusions:
- The test substance is considered as readily biodegradable.
- Executive summary:
The biodegradability was determined with a non adapted activated sludge for the test item over a test period of 28 days in the DOC-Die-Away test. At 7 days, the biodegradation reached the 86% and at 28 days the biodegradation reached the 99%. Therefore, the substance is considered as readily biodegradable.
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- documentation insufficient for assessment
- Principles of method if other than guideline:
- No data provided on the method.
- GLP compliance:
- not specified
- Oxygen conditions:
- not specified
- Inoculum or test system:
- not specified
- Duration of test (contact time):
- 1 - 5 d
- Parameter followed for biodegradation estimation:
- other: BOD (Biological Oxygen Demand)
- Reference substance:
- not specified
- Parameter:
- other: BOD
- Value:
- 79
- Sampling time:
- 1 d
- Parameter:
- other: BOD
- Value:
- 79
- Sampling time:
- 5 d
- Validity criteria fulfilled:
- not applicable
- Interpretation of results:
- readily biodegradable
- Conclusions:
- The biological oxygen demand (BOD) of Ammonium Acetate was 79 % after 1 -5 days.
- Executive summary:
The biological oxygen demand (BOD) of Ammonium Acetate was 79 % after 1 -5 days.
No data provided on test method.
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- documentation insufficient for assessment
- Principles of method if other than guideline:
- No data provided on the method.
- GLP compliance:
- not specified
- Oxygen conditions:
- not specified
- Inoculum or test system:
- not specified
- Duration of test (contact time):
- 1 - 5 d
- Initial conc.:
- 1 000 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- other: BOD (Biological Oxygen Demand)
- Details on study design:
- TEST CONDITIONS
- Test temperature: 20 ºC
- Acclimation time: 1 day - Reference substance:
- not specified
- Parameter:
- other: ThOD
- Value:
- 79
- Sampling time:
- 1 d
- Parameter:
- other: ThOD
- Value:
- 79
- Sampling time:
- 5 d
- Validity criteria fulfilled:
- not applicable
- Interpretation of results:
- readily biodegradable
- Conclusions:
- The biological oxygen demand (BOD) of Ammonium Acetate was 79 % after 1 -5 days at 20 ºC.
- Executive summary:
The biological oxygen demand (BOD) of Ammonium Acetate was 79 % after 1 -5 days at 20 ºC.
No data provided on test method.
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 3 (not reliable)
- Rationale for reliability incl. deficiencies:
- significant methodological deficiencies
- Principles of method if other than guideline:
- Method: bench-scale activated sludge, fill-and-draw operation.
- GLP compliance:
- no
- Oxygen conditions:
- not specified
- Inoculum or test system:
- activated sludge (adaptation not specified)
- Duration of test (contact time):
- >= 1 - <= 5 d
- Initial conc.:
- 1 000 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- other: BOD (Biological Oxygen Demand)
- Details on study design:
- TEST CONDITIONS
- Test temperature: 20 ºC
- Acclimation time: 1 day - Reference substance:
- not specified
- Key result
- Parameter:
- other: ThOD
- Value:
- 79
- Sampling time:
- 1 d
- Key result
- Parameter:
- other: ThOD
- Value:
- 79
- Sampling time:
- 5 d
- Validity criteria fulfilled:
- not applicable
- Interpretation of results:
- readily biodegradable
- Conclusions:
- The biological oxygen demand (BOD) of Ammonium Acetate was 79 % after 1 -5 days at 20 ºC.
- Executive summary:
The biological oxygen demand (BOD) of Ammonium Acetate was 79 % after 1 -5 days at 20 ºC.
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)
- GLP compliance:
- no
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge (adaptation not specified)
- Details on inoculum:
- Quantity of sludge used in the experiment: 3420 ppm
- Duration of test (contact time):
- >= 1 - <= 5 d
- Initial conc.:
- 1 000 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- other: BOD (Biological Oxygen Demand)
- Details on study design:
- TEST CONDITIONS
- Test temperature: 20 ºC
- Acclimation time: 1 day - Reference substance:
- not specified
- Key result
- Parameter:
- other: ThOD
- Value:
- 78.9
- Sampling time:
- 1 d
- Validity criteria fulfilled:
- not applicable
- Interpretation of results:
- readily biodegradable
- Conclusions:
- The biological oxygen demand (BOD) of Ammonium Acetate was 78.9 % after 22-24h.
- Executive summary:
The biological oxygen demand (BOD) of Ammonium Acetate was 78.9 % after 22-24h.
Referenceopen allclose all
The substance Ammonium acetate is considered to be readily biodegradable under test conditions.
The test substance is considered as readily biodegradable.
The biological oxygen demand (BOD) of Ammonium Acetate was 79 % after 1 -5 days.
The biological oxygen demand (BOD) of Ammonium Acetate was 79 % after 1 -5 days at 20 ºC.
The biological oxygen demand (BOD) of Ammonium Acetate was 79 % after 1 -5 days at 20 ºC.
The biological oxygen demand (BOD) of Ammonium Acetate was 78.9 % after 22 -24h.
Description of key information
Biodegradation in water: screening tests: Weight of evidence: The substance Ammonium Acetate is considered as readily biodegradable (experimental results and read-across approach from experimental and estimated data on analogue Sodium Acetate).
Key value for chemical safety assessment
- Biodegradation in water:
- readily biodegradable
Additional information
Experimental data:
The biological oxygen demand (BOD) of Ammonium Acetate was 79 % after 1-5 days at 20 ºC or 78.9% ahter 22 -24h.
Weight of evidence: Experimental results and read-across approach from experimental and estimated data on the analogues Sodium Acetate.
Read-across from experimental data on the analogue Sodium Acetate:
Based on the experimental results obtained with the analogue Sodium acetate, which is considered to be readily biodegradable (> 99% in 28 days), and applying the read-across approach, the substance Ammonium acetate is also considered to be readily biodegradable under test conditions.
Based on all these results, the substance Ammonium Acetate is considered as readily biodegradable.
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