Hydrogen fluoride

Acute Toxicity: other routes

Administrative data

Endpoint:

acute toxicity: other routes

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

2007

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Recent published peer reviewed study

Data source

Reference

Reference Type:

publication

Title:

Harmful effects and acute lethal toxicity of intraveneous administration of low concentrations of hydrofluoric acid in rats

Author:

Mitsui G, Dote T, Adachi K, Dote E, Fujimoto K, Shimbo Y, Fujihara M, Shimizu H, Usuda K & Kono K

Year:

2007

Bibliographic source:

Toxicology and Industrial Health, 23: 5-12

Materials and methods

Test guideline

Qualifier:

no guideline available

Principles of method if other than guideline:

Investigation of acute toxicity using intravenous administration

GLP compliance:

no

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Hydrogen fluoride

EC Number:

231-634-8

EC Name:

Hydrogen fluoride

Cas Number:

7664-39-3

Molecular formula:

FH

IUPAC Name:

hydrogen fluoride

Details on test material:

Hydrofluoric acid (HFA: concentration 46%) obtained from Wako Pure Chemical Industries Ltd., Japan. The chemical was dissolved/diluted in saline.

Test animals

Species:

rat

Strain:

Sprague-Dawley

Sex:

male

Details on test animals or test system and environmental conditions:

Nine week old SPF male Sprague-Dawley rats, weighing 290-300 g. Rat chow (Funabashi Farm MM-3, Japan) and tap water were provided ad libitum. Temperature was maintained at 22±1°C under a 12 hour light/dark cycle.

Administration / exposure

Route of administration:

intravenous

Vehicle:

other: saline

Details on exposure:

Lethal dose study: Rats were anaesthetised with pentobarbital. One of five HFA concentrations (0.3, 0.4, 0.5, 0.6 or 0.7%) was injected through the tail vein by a syringe pump for 1 minute. Total volume injected was 1 ml, and speed of injection was 1 ml/min.
Sublethal acute study: Rats were anaesthetised with pentobarbital. One of four HFA concentrations (0.05, 0.1, 0.2 or 0.3%) was injected through the tail vein by syringe pump. Saline was used as the control. Total volume injected was 1 ml, and speed of injection was 1 ml/min.

Doses:

Lethal dose study: 9.9, 13.1, 16.4, 19.7 and 23.0 mg/kg bw
Sublethal acute study: 0, 1.6, 3.2, 6.4 and 9.6 mg/kg bw

No. of animals per sex per dose:

5 males per group

Control animals:

yes

Details on study design:

Blood samples were obtained from the carotid artery 1 hour after infusion. Aspartate aminotransferase (AST), alanine aminotransferase (ALT), mitochondrial asparate aminotransferase (m-AST), lactate dehydrogenase (LDH), LDH isozyme and serum glucose were measured. Kidney function was determined according to blood urea nitrogen (BUN) and creatinine (Cr).

Statistics:

Probit analysis and ANOVA.

Results and discussion

Effect levels

Effect levels 1

Sex:

male

Dose descriptor:

LCLo

Effect level:

13.1 mg/kg bw

Remarks on result:

other: i.v.

Effect levels 2

Sex:

male

Dose descriptor:

LD50

Effect level:

17.4 mg/kg bw

Remarks on result:

other: i.v.

Effect levels 3

Sex:

male

Dose descriptor:

other: LD90

Effect level:

23 mg/kg bw

Remarks on result:

other: i.v.

Mortality:

13.1 mg/kg resulted in the death of 2/5 rats.

Clinical signs:

Not reported

Body weight:

Not deterined

Gross pathology:

Not examined

Other findings:

BUN was slightly increased in the 1.6 mg/kg group, and was significantly increased in the 3.2, 6.4 and 9.6 mg/kg groups compared with the saline only group. Cr was slightly increased in the 1.6 mg/kg group, and significantly increased in the 3.2, 6.4 and 9.6 mg/kg groups. Serum glucose was significantly increased in the HFA group. There were no other changes in haematology parameters.

Any other information on results incl. tables

No further information

Applicant's summary and conclusion

Conclusions:

The i.v. LDLo was 13.1 mg/kg, and the i.v. LD50 was 17.4 mg/kg.

Executive summary:

The acute toxicity of hydrofluoric acid was determined following intravenous infusion in anaesthetised rats. The i.v. LDLo was 13.1 mg/kg, and the i.v. LD₅₀was 17.4 mg/kg.