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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
acute toxicity: oral
Type of information:
migrated information: read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1978
Report date:
1978

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: Read-across
GLP compliance:
no
Test type:
standard acute method

Test material

Constituent 1
Chemical structure
Reference substance name:
Hexamethylenediamine
EC Number:
204-679-6
EC Name:
Hexamethylenediamine
Cas Number:
124-09-4
Molecular formula:
C6H16N2
IUPAC Name:
hexane-1,6-diamine
Details on test material:
- Name of test material (as cited in study report): hexamethylenediamine
- Molecular formula (if other than submission substance): C6H16N2
- Molecular weight (if other than submission substance): 116.2046
- Smiles notation (if other than submission substance): NCCCCCCN
- InChl (if other than submission substance): InChI=1/C6H16N2/c7-5-3-1-2-4-6-8/h1-8H2
- Structural formula attached as image file (if other than submission substance): see Fig.1
- Substance type: organic
- Physical state: solid

Test animals

Species:
rat
Strain:
Sprague-Dawley
Sex:
male/female
Details on test animals or test system and environmental conditions:
TEST ANIMALS
- Source: ONCINS, IFFA CREDO
- Age at study initiation: no data
- Weight at study initiation: 130 to 160g
- Fasting period before study: no data
- Housing:no data
- Diet (e.g. ad libitum): no data
- Water (e.g. ad libitum): no data
- Acclimation period: no data


ENVIRONMENTAL CONDITIONS
- Temperature (°C): no data
- Humidity (%): no data
- Air changes (per hr): no data
- Photoperiod (hrs dark / hrs light): no data


IN-LIFE DATES: no data

Administration / exposure

Route of administration:
oral: gavage
Vehicle:
unchanged (no vehicle)
Details on oral exposure:
MAXIMUM DOSE VOLUME APPLIED: no data
Doses:
900, 1170, 1530 and 2000mg/kg bw
No. of animals per sex per dose:
5
Control animals:
yes
Details on study design:
- Duration of observation period following administration: 15 days
- Frequency of observations and weighing: animals in the higher non-lethal dose group were weighed once every 5 days
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs
Statistics:
method of Litchfield and Wilcoxon.

Results and discussion

Preliminary study:
No data
Effect levels
Sex:
male/female
Dose descriptor:
LD50
Effect level:
1 160 mg/kg bw
Remarks on result:
other: 95% C.L: no data
Mortality:
See Table 7.2.1/1
Clinical signs:
other: For all doses: cries after treatment and excessive salivation. A decrease of the motor activity was observed at dose level of 1170 and 900 mg/kg bw.
Gross pathology:
At dose levels of 2000 and 1530 mg/kg bw, for all died animals, an ulceration of all the surface of the gastric mucous membrane was noted and also the presence of blood in the intestine was observed.
At dose level of 2000 mg/kg bw, for the surviving animal, an ulceration of the 3/4 mucous membrane was observed, with a thickening and an adherence to spleen, stomach and abdominal wall.
At dose level of 1530 mg/kg bw, one surviving animal presented the same lesions of the surviving animal at dose level of 2000 mg/kg bw, and the other surviving animal not presented any lesion.
At dose level of 1170 mg/kg bw, for the 7 died animals, an ulceration of the pylore was noted. No lesions were observed for the surviving animals.
At dose level of 900 mg/kg bw, no lesions were observed.
Other findings:
No data

Any other information on results incl. tables

Table 7.2.1/1 Number of animals dead and time range within which mortality occured

Dose

(mg/kg/bw)

Mortality (# dead/total)

Time range o f deaths (hours)

Male

Female

Combined

Control

-

-

-

-

900

-

-

01/10

D6

1170

-

-

07/10

D2-D4

1530

-

-

08/10

D0-D12

2000

-

-

09/10

D0-D4

Applicant's summary and conclusion

Interpretation of results:
harmful
Remarks:
Migrated information Criteria used for interpretation of results: EU