Calcium 3-hydroxy-4-[(4-methyl-2-sulphonatophenyl)azo]-2-naphthoate

Toxicity to soil macroorganisms except arthropods

Administrative data

Endpoint:

toxicity to soil macroorganisms except arthropods: short-term

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2007

Report date:

2007

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 207 (Earthworm, Acute Toxicity Tests)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Calcium 4-[(5-chloro-4-methyl-2-sulphonatophenyl)azo]-3-hydroxy-2-naphthoate

EC Number:

230-303-5

EC Name:

Calcium 4-[(5-chloro-4-methyl-2-sulphonatophenyl)azo]-3-hydroxy-2-naphthoate

Cas Number:

7023-61-2

Molecular formula:

C18H13ClN2O6S.Ca

IUPAC Name:

calcium 4-[(5-chloro-4-methyl-2-sulfonatophenyl)diazenyl]-3-hydroxy-2-naphthoate

Test material form:

solid: nanoform

Specific details on test material used for the study:

- Storage condition of test material: at room temperature at abput 20 °C, away from direct sunlight

Sampling and analysis

Analytical monitoring:

no

Test substrate

Vehicle:

no

Details on preparation and application of test substrate:

- Method of mixing into soil: the test substance was mixed directly with a portion of sand prior to addition to the artificial soil. For the preparation of the sand/test item mixtures, the amounts of 34.8, 69.5, 139, 278 and 556 mg of the test were weighed by an analytical balance and mixed into 10 g of sand each by means of a roller mixer. The sand/test item mixtures were prepared immediately before the start of the test. Each replicate was quantitatively incorporated into 556 g of artificial soil (dry weight) by intensely mixing.

Test organisms

Test organisms (species):

Eisenia fetida

Animal group:

annelids

Details on test organisms:

TEST ORGANISM
- Common name: earthworm
- Source: obtained from a breeding stock maintained at RCC
- Age at test initiation: adults with clitellum approx. 4 months old
- Weight at test initiation (mean range): 350 - 432 mg

HOUSING
- Feeding: horse manure, potatoes

ACCLIMATION
- Acclimation period: one day
- Acclimation conditions: artificial soil and test temperature

Study design

Study type:

laboratory study

Substrate type:

artificial soil

Limit test:

no

Total exposure duration:

14 d

Test conditions

Test temperature:

18 - 21 °C

pH:

- Initial pH = 5.8
- pH at test end: 5.9 - 6.2

Moisture:

- Initail moisture content: 35%
- Moisture at test end: 33-34%

Details on test conditions:

TEST SYSTEM
- Test container: cylindrical glass vessels (diameter 10 cm, height 14 cm) covered with glass lids
- Amount of soil or substrate: 556 g artificial soil dry weight
- No. of organisms per container (treatment): 10
- No. of replicates per treatment group: 5
- No. of replicates per control: 5

OTHER TEST CONDITIONS
- Photoperiod: coninuous illumination
- Light intensity: 526 - 631 lux

EFFECT PARAMETERS MEASURED:
- Mortality and symptoms of toxicity: after 7 and 14 days
- Body weight after: 0 and 14 days
- Soil moisture after: 0 and 14 days
- pH: after 0 and 14 days
- Temperature: continuously

TEST CONCENTRATIONS
- Range finding study: yes

Nominal and measured concentrations:

Nominal test concentrations: control, 62.5, 125, 250, 500, 1000 mg/kg dry soil (based on range-finding study)

Reference substance (positive control):

yes

Remarks:

2-chloroacetamide (test is performed once a year)

Results and discussion

Effect concentrations

Effect concentrations 1

Key result

Duration:

14 d

Dose descriptor:

LC50

Effect conc.:

> 1 000 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Effect concentrations 2

Duration:

14 d

Dose descriptor:

LC0

Effect conc.:

>= 1 000 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Effect concentrations 3

Duration:

14 d

Dose descriptor:

LC100

Effect conc.:

> 1 000 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Details on results:

- Mortality at end of exposure period: none
- Abnormal behaviour: none
- Symptoms of toxicity: none
- Body weight: not affected

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

No toxic effects have been observed in studies with soil living organisms.