Hydrocarbons, C4, 1,3-butadiene-free, polymd., triisobutylene fraction

Toxicity to soil macroorganisms except arthropods

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

toxicity to soil macroorganisms except arthropods: long-term

Type of information:

(Q)SAR

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

results derived from a valid (Q)SAR model and falling into its applicability domain, with adequate and reliable documentation / justification

Justification for type of information:

See Attached Justification

Qualifier:

according to guideline

Guideline:

other: REACH Guideance on QSARs

Principles of method if other than guideline:

- Software tool(s) used including version:
See Field 'Attached justification'
- Model(s) used:
See Field 'Attached justification'
- Model description: See Field 'Attached justification'
- Justification of QSAR prediction: see field 'Attached justification'

GLP compliance:

not specified

Specific details on test material used for the study:

HC Block Type Carbon Number % weight
i-Olefin 12 100

Total 100

Analytical monitoring:

not required

Test organisms (species):

Eisenia fetida

Animal group:

annelids

Study type:

other: QSAR

Reference substance (positive control):

no

Remarks:

Not Required

Duration:

14 d

Dose descriptor:

other: LL50

Effect conc.:

5 862 mg/kg soil dw

Nominal / measured:

estimated

Conc. based on:

other: QSAR prediction

Basis for effect:

mortality

Remarks on result:

other: QSAR predicted value

Duration:

56 d

Dose descriptor:

other: EL10

Effect conc.:

1 123 mg/kg soil dw

Nominal / measured:

estimated

Conc. based on:

other: QSAR prediction

Basis for effect:

reproduction

Remarks on result:

other: QSAR predicted value

Validity criteria fulfilled:

yes

Conclusions:

The QSAR predicted LL50 and EL10 for the UVCB substance were 5862 and 1123 mg/kg soil dw respectively.

Executive summary:

The QSAR predicted LL50 and EL10 for the UVCB substance for Eisenia fetida were 5862 and 1123 mg/kg soil dw, respectively. The model used for the calculations was the PETROTOX v4.0 model. PETROTOX is a well-documented and peer reviewed model that is widely used across the chemicals industry. This substance fits within the criteria of the model and there are no reservations about the validity of the model runs. It is expected that this data is reliable with restrictions.

Reference 2

Endpoint:

toxicity to soil macroorganisms except arthropods: long-term

Type of information:

(Q)SAR

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

results derived from a valid (Q)SAR model and falling into its applicability domain, with adequate and reliable documentation / justification

Justification for type of information:

See Attached Justification

Qualifier:

according to guideline

Guideline:

other: REACH Guideance on QSARs

Principles of method if other than guideline:

- Software tool(s) used including version:
See Field 'Attached justification'
- Model(s) used:
See Field 'Attached justification'
- Model description: See Field 'Attached justification'
- Justification of QSAR prediction: see field 'Attached justification'

GLP compliance:

not specified

Specific details on test material used for the study:

HC Block Type Carbon Number % weight
i-Olefin 16 100

Total 100

Analytical monitoring:

not required

Test organisms (species):

Eisenia fetida

Animal group:

annelids

Study type:

other: QSAR

Reference substance (positive control):

no

Remarks:

Not Required

Duration:

14 d

Dose descriptor:

other: LL50

Effect conc.:

1 271 117 mg/kg soil dw

Nominal / measured:

estimated

Conc. based on:

other: QSAR prediction

Basis for effect:

mortality

Remarks on result:

other: QSAR predicted value

Duration:

56 d

Dose descriptor:

other: EL10

Effect conc.:

243 509 mg/kg soil dw

Nominal / measured:

estimated

Conc. based on:

other: QSAR prediction

Basis for effect:

reproduction

Remarks on result:

other: QSAR predicted value

Validity criteria fulfilled:

yes

Conclusions:

The QSAR predicted LL50 and EL10 for the UVCB substance were 1271117 and 243509 mg/kg soil dw respectively.

Executive summary:

The QSAR predicted LL50 and EL10 for the UVCB substance for Eisenia fetida were 1271117 and 243509 mg/kg soil dw, respectively. The model used for the calculations was the PETROTOX v4.0 model. PETROTOX is a well-documented and peer reviewed model that is widely used across the chemicals industry. This substance fits within the criteria of the model and there are no reservations about the validity of the model runs. It is expected that this data is reliable with restrictions.

Reference 3

Endpoint:

toxicity to soil macroorganisms except arthropods: long-term

Type of information:

(Q)SAR

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

results derived from a valid (Q)SAR model and falling into its applicability domain, with adequate and reliable documentation / justification

Justification for type of information:

See Attached Justification

Qualifier:

according to guideline

Guideline:

other: REACH Guideance on QSARs

Principles of method if other than guideline:

- Software tool(s) used including version:
See Field 'Attached justification'
- Model(s) used:
See Field 'Attached justification'
- Model description: See Field 'Attached justification'
- Justification of QSAR prediction: see field 'Attached justification'

GLP compliance:

not specified

Specific details on test material used for the study:

HC Block Type Carbon Number % weight
i-Olefin 20 100

Total 100

Analytical monitoring:

not required

Test organisms (species):

Eisenia fetida

Animal group:

annelids

Study type:

other: QSAR

Reference substance (positive control):

no

Remarks:

Not Required

Duration:

28 d

Dose descriptor:

other: LL50

Effect conc.:

1 271 117 mg/kg soil dw

Nominal / measured:

estimated

Conc. based on:

other: QSAR prediction

Basis for effect:

mortality

Remarks on result:

other: QSAR predicted value

Duration:

56 d

Dose descriptor:

other: EL10

Effect conc.:

243 509 mg/kg soil dw

Nominal / measured:

estimated

Conc. based on:

other: QSAR prediction

Basis for effect:

reproduction

Remarks on result:

other: QSAR predicted value

Validity criteria fulfilled:

yes

Conclusions:

The QSAR predicted LL50 and EL10 for the UVCB substance were 1271117 and 243509 mg/kg soil dw respectively.

Executive summary:

The QSAR predicted LL50 and EL10 for the UVCB substance for Eisenia fetida were1271117 and 243509 mg/kg soil dw, respectively. The model used for the calculations was the PETROTOX v4.0 model. PETROTOX is a well-documented and peer reviewed model that is widely used across the chemicals industry. This substance fits within the criteria of the model and there are no reservations about the validity of the model runs. It is expected that this data is reliable with restrictions.

Reference 4

Endpoint:

toxicity to soil macroorganisms except arthropods: long-term

Type of information:

(Q)SAR

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

results derived from a valid (Q)SAR model and falling into its applicability domain, with adequate and reliable documentation / justification

Justification for type of information:

See Attached Justification

Qualifier:

according to guideline

Guideline:

other: REACH Guideance on QSARs

Principles of method if other than guideline:

- Software tool(s) used including version:
See Field 'Attached justification'
- Model(s) used:
See Field 'Attached justification'
- Model description: See Field 'Attached justification'
- Justification of QSAR prediction: see field 'Attached justification'

GLP compliance:

not specified

Specific details on test material used for the study:

HC Block Type Carbon Number % weight
i-Olefin 8 100

Total 100

Analytical monitoring:

not required

Test organisms (species):

Eisenia fetida

Animal group:

annelids

Study type:

other: QSAR

Reference substance (positive control):

no

Remarks:

Not Required

Duration:

14 d

Dose descriptor:

other: LL50

Effect conc.:

217 mg/kg soil dw

Nominal / measured:

estimated

Conc. based on:

other: QSAR prediction

Basis for effect:

mortality

Remarks on result:

other: QSAR predicted value

Duration:

56 d

Dose descriptor:

other: EL10

Effect conc.:

41.5 mg/kg soil dw

Nominal / measured:

estimated

Conc. based on:

other: QSAR prediction

Basis for effect:

reproduction

Remarks on result:

other: QSAR predicted value

Validity criteria fulfilled:

yes

Conclusions:

The QSAR predicted LL50 and EL10 for the UVCB substance were 217 and 41.5 mg/kg soil dw respectively.

Executive summary:

The QSAR predicted LL50 and EL10 for the UVCB substance for Eisenia fetida were 217 and 41.5  mg/kg soil dw, respectively. The model used for the calculations was the PETROTOX v4.0 model. PETROTOX is a well-documented and peer reviewed model that is widely used across the chemicals industry. This substance fits within the criteria of the model and there are no reservations about the validity of the model runs. It is expected that this data is reliable with restrictions.

Reference 5

Endpoint:

toxicity to soil macroorganisms except arthropods: short-term

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1st October 2008 - 29th Janurary 2009

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: GLP compliant, guideline study, available as an unpublished report, reliable with restrictions

Qualifier:

according to guideline

Guideline:

OECD Guideline 207 (Earthworm, Acute Toxicity Tests)

Deviations:

no

Qualifier:

according to guideline

Guideline:

ISO 11268-1 (Effects of Pollutants on Earthworms. 1. Determination of Acute Toxicity Using Artificial Soil Substrate)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
No data

Analytical monitoring:

no

Details on sampling:

Live weight of earthworms was measured individually on the day of application (day 0) and on day 14. Mortality, behaviour and morphological changes of earthworms were recorded 7 and 14 days after application.

Vehicle:

no

Details on preparation and application of test substrate:

Test item was weighed and mixed with quartz sand (10g quartz sand per replicate). Treated quartz sand was added to artificial soil. Demineralised water was added to adjust the humidity of the artificial soil to 44% of the maximum water holding capacity. Test medium was mixed for > 3minutes and added to test vessels.

Test organisms (species):

Eisenia fetida

Animal group:

annelids

Details on test organisms:

Source: Eisena fetida are bred at the test facility.
Holding conditions prior to use: Kept in covered plastic vessels (Volume 60 L) containing potting compost. Kept at 20 +/- 2°C in the dark and fed with dried litter of stinging nettles and porridge oats
Individual weight of test organisms: between 0.30 and 0.60g

Study type:

laboratory study

Substrate type:

artificial soil

Limit test:

no

Total exposure duration:

14 d

Post exposure observation period:

No data

Test temperature:

18 - 22°C

pH:

Day 0: 5.10 - 5.27
Day 14: 4.90 - 5.19

Moisture:

Day 0: 30.2 - 31.6 % of dry weight
30.7 - 31.6% of dry weight

Details on test conditions:

Photoperiod: 16 hours
Light intensity: 400 - 800 lx
Feeding: No feeding during the toxicity test

Nominal and measured concentrations:

Nominal concentrations: 62.5, 125, 250, 500, 1000 mg/kg soil dry weight

Reference substance (positive control):

yes

Remarks:

2-chloroacetamide

Duration:

14 d

Dose descriptor:

NOEC

Effect conc.:

1 000 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks:

Mortality, loss of body weight and other symptoms (behaviour and pathological symptoms) were all tested

Details on results:

No significant mortality, loss of body weight or pathological or bahavioural symptoms were observed in any test concentration over the course of the exposure period. Therefore the lowest test item concentartion with significant effects is > 1000 mg/kg soil dw.

Results with reference substance (positive control):

No data

Reported statistics and error estimates:

No data

Validity criteria fulfilled:

yes

Remarks:

Mortality in the control group did not exceed 10%. Average loss of biomass in the control group did not exceed 20%.

Conclusions:

The 14 day toxicity study with the earthworm Eisenia fetida measured a NOEC of 1000 mg/kg soil dw for 9003-26-6. No significant mortality, loss of body weight or pathological ot behavioural symptoms were measured at any of the tested concentrations.

Executive summary:

This is a GLP, guideline study and is considered reliable and suitable for use for this endpoint. The 14 day toxicity study with the earthworm Eisenia fetida measured a NOEC of 1000 mg/kg soil dw for 9003 -26 -6. No significant mortality, loss of body weight or pathological ot behavioural symptoms were measured at any of the tested concentrations.

Description of key information

In a proprietary, GLP compliant, guideline study the 14 day NOEC of Polybutene (CAS 9003-29-6) to Eisenia fetida was found to be 1000 mg/kg soil. Polybutene is a representative of Category B.

In addition to the experimental data, the terrestrial toxicity was predicted by a QSAR, the PETROTOX v4.0 computer model. PETROTOX is a well-documented and peer reviewed model that is widely used across the chemicals industry. The predicted LL50 values for Eisenia fetida ranged from 217 to 1271117 mg/kg soil dw. The predicted EL10 values for Eisenia fetida ranged from 41.5 to 243509 mg/kg soil dw.

Additional information

Experimental data:

CAS 9003-29-6: the 14 day NOEC of Polybutene to Eisenia fetida was found to be 1000 mg/kg soil.

 

QSAR predictions:

 

The PETROTOX v4.0 model for sediment terrestrial organism calculates the constituent concentration in sediment or soil using the product composition (% mass) along with the total sediment/soil (s/s) loading rate (concentration of the full substance as added to the soil/sediment). The model then uses the equilibrium-partitioning calculations to convert aqueous-based effect concentrations (EC50, mmol/L) to soil or sediment based values (mmol/kg oc) for each constituent using the CTLBB for the soil or sediment organism. Then, it combines the s/s based concentration (mg/kg dw) and the effect concentrations in soil or sediment based values (mmol/kg oc) to compute toxic units for each constituent, which are then summed to determine the overall effect concentration (LC50ss) of the substance.

 

The predicted values were:

Species	Carbon No.	LL50 / EL50 (mg/kg_dw)	EL10 (mg/kg_dw)
Eisenia fetida	C8	217	41.5
Eisenia fetida	C12	5862	1123
Eisenia fetida	C16	1271117	243509
Eisenia fetida	C20	1271117	243509