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EC number: 203-474-9 | CAS number: 107-22-2
Toxicological information
Acute Toxicity: oral
Currently viewing:
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1984
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Referenceopen allclose all
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 984
- Report date:
- 1984
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 008
- Report date:
- 2008
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 401 (Acute Oral Toxicity)
- Version / remarks:
- (1981)
- Qualifier:
- according to guideline
- Guideline:
- other: EEC Directive 79-831, Annex V, Part B: Methods For The Determination Of Toxicity, 4.1.1 Acute Toxicity Orally
- GLP compliance:
- yes
- Test type:
- standard acute method
- Limit test:
- no
Test material
- Reference substance name:
- Glyoxal
- EC Number:
- 203-474-9
- EC Name:
- Glyoxal
- Cas Number:
- 107-22-2
- Molecular formula:
- C2H2O2
- IUPAC Name:
- oxalaldehyde
- Test material form:
- liquid
Constituent 1
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: Code HF 0001
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: in the dark, at room temperature
- Solubility and stability of the test substance in the solvent/vehicle: Glyoxal as 40% aqueous solution is stable for 6 months
OTHER
- Impurities (identity and concentrations): acetic acid, formic acid, glycolic acid, glyoxylic acid, nitric acid, formaldehyde, acetaldehyde, glycol aldehyde, ethylene glycol
Test animals
- Species:
- rat
- Strain:
- Wistar
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Hoechst AG, Kastengrund, SPF-Zucht
- Weight at study initiation: males, 190.3 g (182 - 203 g); females, 177.9 g (165 - 193 g)
- Fasting period before study: 16 hours prior and 2 hours after treatment
- Housing: five animals per cage, in Makrolon cages
- Diet (e.g. ad libitum): rat feed (Rattendiaet Altromin 1324), ad libitum
- Water (e.g. ad libitum): Tap water, ad libitum
- Acclimation period: at least 5 days
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22 +/- 2
- Humidity (%): 55 +/- 10
- Air changes (per hr): housing in fully air-conditioned rooms
- Photoperiod (hrs dark / hrs light): 12 h/ 12 h
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- water
- Details on oral exposure:
- - The concentration of test substance in vehicle was 25 % as w/v, for each dose level
- The application volume was 8, 12.6 and 20 mL/kg bw for the 2000, the 3150 and the 5000 mg/kg bw dose level, respectively - Doses:
- 2000, 3150 and 5000 mg/kg bw of test substance containing 40% a.i., i.e. 800, 1260 and 2000 mg/kg bw of active ingredient
- No. of animals per sex per dose:
- 10 animals/sex/dose
- Control animals:
- no
- Details on study design:
- - The treatment was followed by a 14-day post -exposure period of observation;
- The animals were observed for mortality and clinical symptoms of toxicity several times, as recommended by the OECD guideline;
- Body weight was recorded once a week;
- Animals that died during the 14-day observation period were subjected to necropsy and were examined for gross pathology;
- At the end of the observation period, the surviving animals were sacrificed for the purpose of necropsy. - Statistics:
- The determination of the LD50 was based on the Probit Analysis
Results and discussion
Effect levelsopen allclose all
- Key result
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- 3 300 mg/kg bw
- Sex:
- male
- Dose descriptor:
- LD50
- Effect level:
- 3 660 mg/kg bw
- Sex:
- female
- Dose descriptor:
- LD50
- Effect level:
- 2 960 mg/kg bw
- Mortality:
- - In the 2000 mg/kg bw group: 0/5 males 1/5 females (i.e. 10% mortality)
- In the 3150 mg/kg bw group: 2/5 males 2/5 females (i.e. 40% mortality)
- In the 5000 mg/kg bw group: 4/5 males 5/5 females (i.e. 90% mortality)
- All cases of death occurred between 70 minutes and 24 hours following dosing. - Clinical signs:
- other: - Clinical symptoms indicative of toxicity were seen in all groups and both sexes. - Following treatment, the animals showed decreased spontaneous activity, decreased respiration rate, increased water consumption, uncoordinated gait, squatting position,
- Gross pathology:
- - Necropsy of animals that died: partly firm small and large intestines filled with a colorless liquid, some cases of reddening of the stomach mucosa, spotted liver and dark discoloration of the adrenals, some cases of increased blood content in the lung, and some cases of grey-pink discoloration of the lung were reported.
- Necropsy of animals that survived and were sacrificed at the end of the observation period: no abnormalities were reported.
Applicant's summary and conclusion
- Interpretation of results:
- GHS criteria not met
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