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Environmental fate & pathways

Biodegradation in water: screening tests

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Link to relevant study record(s)

Description of key information

The substance is not readily biodegradable.

Key value for chemical safety assessment

Biodegradation in water:
under test conditions no biodegradation observed

Additional information

In an Appendix to the laboratory study entitled “The degree of Accumulation Test in Carp: 3,3',3'-tris (dimethylamino) tripropylamine “ (Mitsubishi Kasei Institute of Toxicological and Environmental Sciences, 1981), a ready biodegradability test similar to if not following the OECD 301C (Ministry of International Trade and Industry, Japan) Guideline was performed to determine the biodegradability of PolyCat 9. The biodegradability study was performed 10 March 1981 to 07 April 1981 at the Mitsubishi Kasei Institute of Toxicological and Environmental Sciences for the study sponsor San-Abbot Ltd. The oxygen uptake of the test substance, PolyCat 9 inoculated with standard activated sludge, was measured over a period of 28 days at a temperature of 25 ±1°C. Biodegradation was expressed as the percentage oxygen uptake (corrected for blank uptake) of the theoretical uptake (ThOD). The test used 6 bottles, 300 ml each of the test liquid. Bottle #1 acted as the sludge activity check or the functional control and contained 101 mg/L aniline and 30 mg/L activated sludge. Bottles #2, #3, and #4 contained the test substance PolyCat 9 at a concentration of 100 mg/L with a concentration of activated sludge at 30 mg/L. Bottle #5 acted as the abiotic control and contained the blank sample of PolyCat 9 at a concentration of 100 g/L and no activated sludge. Bottle #6 acted as the inoculum control or sludge blank and contained 30 mg/L activated sludge. The mean % Biodegradation of PolyCat 9 for the 3 test replicates was 2.4% after the 28 d test duration with individual results of 0.9% for Sample #2, 5.5% for Sample #3 and 0.8% for Sample #4. PolyCat 9 did not reach pass levels for biodegradation during the 28 d test. Therefore, PolyCat 9 is not readily biodegradable