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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
sediment toxicity: long-term
Data waiving:
study scientifically not necessary / other information available
Justification for data waiving:
other:
Cross-referenceopen allclose all
Reason / purpose for cross-reference:
data waiving: supporting information
Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
supporting study
Study period:
2014
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study with acceptable restrictions
Qualifier:
according to guideline
Guideline:
other: EPA 821/R-02/012
Version / remarks:
Methods for measuring the acute toxicity of effluents and receiving waters to freshwater and marine organisms, 5th ed
GLP compliance:
not specified
Specific details on test material used for the study:
no data
Analytical monitoring:
yes
Details on sampling:
- Sampling method: Experimental water was collected from serial dilutions 1 to 3 times per experiment
- Sample storage conditions before analysis: Anion samples were transported in bicarbonate-rinsed 125-mL polyethylene containers and refrigerated. Cation samples were held in 125-mL I-ChemTM containers and preserved at pH < 2.0. All water samples were refrigerated and sent to the Montana Department of Health and Human Services laboratory in Helena, Montana (USA), for analyses.
Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: exposure water containing increasing concentrations of NaHCO3 was added to create a range of exposures from a control to up to 15910 mg/L NaHCO3, depending on the sensitivity of the organism determined during range-finding experiment. Serial dilutions of stock solution were used to create the exposure concentration ranges.
Test organisms (species):
Chironomus dilutus
Details on test organisms:
TEST ORGANISM
- Common name: chiromids
- Strain: native to the Powder and Tongue River drainages
- Age at study initiation (mean and range, SD): 7-14 dph

Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Test temperature:
21–23 C
Nominal and measured concentrations:
295–15 910 mg/L NaHCO3
Details on test conditions:
TEST SYSTEM
- Test vessel: 0.5- L to 4-L acid-washed glass aquaria
- Renewal rate of test solution (frequency): water replaced every 24h
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Tongue and Powder rivers reconstituted waters (check table in other info on methodfor water description). The reconstituted waters were created using a combination of well water from the Jackson Field Research Station, Jackson, Wyoming (USA) and the addition of chemicals of American Chemical Society (ACS) grade or higher or were created at the Montana Fish Wildlife and Parks, Helena, Montana (USA) laboratory using deionized water and chemicals of ACS grade or higher.

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : median lethal concnetration

TEST CONCENTRATIONS
- Results from range finding used to determine the conditions for the definitive study: yes
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
4 947 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other:
Remarks:
in Toungue water
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
8 014 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other:
Remarks:
in Powder recon water
Details on results:
no data
Results with reference substance (positive control):
not applicable
Reported statistics and error estimates:
Median lethal concentrations were calculated with the Toxicity Relationship Analysis Program (TRAP) using measured NaHCO3 and HCO3 values. The predicted toxicity of salt combinations in experimental waters was tested on fathead minnows and C. dubia using the salinity toxicity relationship (STR) model
Validity criteria fulfilled:
not specified
Conclusions:
Short-term exposure of 7-14 dph chironomids to NaHCO3 resulted in a LC50 of 4947 or 8014 mg/l depending on the medium (reconstituted river water) used.
Executive summary:

A study was conducted exposing for 96 h, embryos (7 -14 dph) of chironomids, Chironomus dilutus, to a range concentration 295–15910 mg/L of NaHCO3. Experiments were conducted following the standard EPA guideline 821/R-02/012“Methods for measuring the acute toxicity of effluents and receiving waters to freshwater and marine organisms, 5th ed”, in two different reconstituted river waters (Tongue and Powder). Results showed an LC50 of 4947 or 8014 mg/l NaHCO3, when expose in Tongue or Powder river water, respectively.

Reason / purpose for cross-reference:
data waiving: supporting information
Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
supporting study
Study period:
2014
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study with acceptable restrictions
Qualifier:
according to guideline
Guideline:
other: EPA 821/R-02/012
Version / remarks:
Methods for measuring the acute toxicity of effluents and receiving waters to freshwater and marine organisms, 5th ed
GLP compliance:
not specified
Specific details on test material used for the study:
no data
Analytical monitoring:
yes
Details on sampling:
- Sampling method: Experimental water was collected from serial dilutions 1 to 3 times per experiment
- Sample storage conditions before analysis: Anion samples were transported in bicarbonate-rinsed 125-mL polyethylene containers and refrigerated. Cation samples were held in 125-mL I-ChemTM containers and preserved at pH < 2.0. All water samples were refrigerated and sent to the Montana Department of Health and Human Services laboratory in Helena, Montana (USA), for analyses.
Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: exposure water containing increasing concentrations of NaHCO3 was added to create a range of exposures from a control to up to 1794 mg/L NaHCO3, depending on the sensitivity of the organism determined during range-finding experiment. Serial dilutions of stock solution were used to create the exposure concentration ranges.
Test organisms (species):
other aquatic crustacea: Hyalella azteca
Details on test organisms:
TEST ORGANISM
- Common name: amphipod
- Strain: native to the Powder and Tongue River drainages
- Age at study initiation (mean and range, SD): adults

Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Test temperature:
23 C
Nominal and measured concentrations:
255–1794 mg/L NaHCO3
Details on test conditions:
TEST SYSTEM
- Test vessel: 0.5- L to 4-L acid-washed glass aquaria
- Renewal rate of test solution (frequency): water replaced every 24h
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Tongue and Powder rivers reconstituted waters (check table in other info on methodfor water description). The reconstituted waters were created using a combination of well water from the Jackson Field Research Station, Jackson, Wyoming (USA) and the addition of chemicals of American Chemical Society (ACS) grade or higher or were created at the Montana Fish Wildlife and Parks, Helena, Montana (USA) laboratory using deionized water and chemicals of ACS grade or higher.

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : median lethal concnetration

TEST CONCENTRATIONS
- Results from range finding used to determine the conditions for the definitive study: yes
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
1 419 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other:
Remarks:
in Toungue water
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
2 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other:
Remarks:
in Powder recon water
Details on results:
no data
Results with reference substance (positive control):
not applicable
Reported statistics and error estimates:
Median lethal concentrations were calculated with the Toxicity Relationship Analysis Program (TRAP) using measured NaHCO3 and HCO3 values. The predicted toxicity of salt combinations in experimental waters was tested on fathead minnows and C. dubia using the salinity toxicity relationship (STR) model
Validity criteria fulfilled:
not specified
Conclusions:
Short-term exposure of adults amphipod to NaHCO3 resulted in a LC50 of 1419 or 2000 mg/l depending on the medium (reconstituted river water) used.
Executive summary:

A study was conducted exposing for 96 h adults amphipod, Hyalella azteca, to a range concentration 255–1794 mg/L of NaHCO3. Experiments were conducted following the standard EPA guideline 821/R-02/012“Methods for measuring the acute toxicity of effluents and receiving waters to freshwater and marine organisms, 5th ed”, in two different reconstituted river waters (Tongue and Powder). Results showed an LC50 of 1419 or 2000 mg/l NaHCO3, when expose in Tongue or Powder river water, respectively.

Reason / purpose for cross-reference:
data waiving: supporting information
Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
supporting study
Study period:
2014
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study with acceptable restrictions
Qualifier:
according to guideline
Guideline:
other: EPA 821/R-02/012
Version / remarks:
Methods for measuring the acute toxicity of effluents and receiving waters to freshwater and marine organisms, 5th ed
GLP compliance:
not specified
Specific details on test material used for the study:
no data
Analytical monitoring:
yes
Details on sampling:
- Sampling method: Experimental water was collected from serial dilutions 1 to 3 times per experiment
- Sample storage conditions before analysis: Anion samples were transported in bicarbonate-rinsed 125-mL polyethylene containers and refrigerated. Cation samples were held in 125-mL I-ChemTM containers and preserved at pH < 2.0. All water samples were refrigerated and sent to the Montana Department of Health and Human Services laboratory in Helena, Montana (USA), for analyses.
Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: exposure water containing increasing concentrations of NaHCO3 was added to create a range of exposures from a control to up to 5060 mg/L NaHCO3, depending on the sensitivity of the organism determined during range-finding experiment. Serial dilutions of stock solution were used to create the exposure concentration ranges.
Test organisms (species):
other aquatic worm: Tubifex tubifex
Details on test organisms:
TEST ORGANISM
- Common name: tubifex worm
- Strain: native to the Powder and Tongue River drainages
- Age at study initiation (mean and range, SD): adults

Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Test temperature:
17-19 C
Nominal and measured concentrations:
319–5060 mg/L NaHCO3
Details on test conditions:
TEST SYSTEM
- Test vessel: 0.5- L to 4-L acid-washed glass aquaria
- Renewal rate of test solution (frequency): water replaced every 24h
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Tongue and Powder rivers reconstituted waters (check table in other info on methodfor water description). The reconstituted waters were created using a combination of well water from the Jackson Field Research Station, Jackson, Wyoming (USA) and the addition of chemicals of American Chemical Society (ACS) grade or higher or were created at the Montana Fish Wildlife and Parks, Helena, Montana (USA) laboratory using deionized water and chemicals of ACS grade or higher.

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : median lethal concnetration

TEST CONCENTRATIONS
- Results from range finding used to determine the conditions for the definitive study: yes
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
3 297 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other:
Remarks:
in Toungue water
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
3 367 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other:
Remarks:
in Powder recon water
Details on results:
no data
Results with reference substance (positive control):
not applicable
Reported statistics and error estimates:
Median lethal concentrations were calculated with the Toxicity Relationship Analysis Program (TRAP) using measured NaHCO3 and HCO3 values. The predicted toxicity of salt combinations in experimental waters was tested on fathead minnows and C. dubia using the salinity toxicity relationship (STR) model
Validity criteria fulfilled:
not specified
Conclusions:
Short-term exposure of adults tubifex worms to NaHCO3 resulted in a LC50 of 3297 or 3367 mg/l depending on the medium (reconstituted river water) used.
Executive summary:

A study was conducted exposing for 96 h adults of worm, Tubifex tubifex, to a range concentration 319–5060 mg/L of NaHCO3. Experiments were conducted following the standard EPA guideline 821/R-02/012“Methods for measuring the acute toxicity of effluents and receiving waters to freshwater and marine organisms, 5th ed”, in two different reconstituted river waters (Tongue and Powder). Results showed an LC50 of 3297 or 3367 mg/l NaHCO3, when expose in Tongue or Powder river water, respectively.

Data source

Materials and methods

Test material

Constituent 1
Chemical structure
Reference substance name:
Sodium hydrogencarbonate
EC Number:
205-633-8
EC Name:
Sodium hydrogencarbonate
Cas Number:
144-55-8
Molecular formula:
CH2O3.Na
IUPAC Name:
sodium hydrogen carbonate

Results and discussion

Applicant's summary and conclusion