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Toxicity to terrestrial arthropods

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Endpoint:
toxicity to terrestrial arthropods: short-term
Type of information:
experimental study
Adequacy of study:
key study
Study period:
07 June - 09 August 2006
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP guideline study
Qualifier:
according to
Guideline:
EPA OPPTS 850.3020 (Honey Bee Acute Contact Toxicity)
Deviations:
no
GLP compliance:
yes
Remarks:
no attest of authority; California Agricultural Research Inc., Kerman, CA, USA
Application method:
contact
Analytical monitoring:
no
Vehicle:
yes
Details on preparation and application of test substrate:
- Method of test material application: single topical dose
- Body part: thorax or abdomen
- Volume of test solution applied: 5 µL
- Controls: untreated and with 5 µL sterile water
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): sterile water
Test organisms (species):
Apis mellifera
Animal group:
Hymenoptera (honeybees)
Details on test organisms:
TEST ORGANISM
- Common name: honey bee, Italien strain
- Source: California Agricultural Reserach (CAR) Inc. bee yard (hives originally created in 2000 from packaged bees shipped by Bear River Honey Company, Wheatland, CA.)
- Age at test initiation (mean and range, SD): 1 to 5 days
- Cultural background (if honeybees):
- Disease free: yes
- Kept according to standard practices: yes


ACCLIMATION
- Acclimation: CAR bee yard; prior to study start all hives were inventoried for their general health, vigor, and brood conditions
- Health during acclimation (any mortality observed): no
Study type:
laboratory study
Limit test:
yes
Total exposure duration:
48 h
Post exposure observation period:
4, 6, 24, and 48 h
Test temperature:
26.73° to 35.79°C
Humidity:
31% - 74%
Details on test conditions:
TEST SYSTEM
- Test container (material, size): cube-shaped, wire mesh cages, appr. 12.7 cm to a side, constructed of 3.2 mesh/cm2 hardware cloth
- Amount of soil or substrate:
- No. of organisms per container (treatment): 40 - 45
- No. of replicates per treatment group: 4
- No. of replicates per control: 4
- No. of replicates per vehicle control: 4


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mortality


VEHICLE CONTROL PERFORMED: yes
Nominal and measured concentrations:
25 µL a.i./bee
Reference substance (positive control):
no
Duration:
48 h
Dose descriptor:
LD0
Effect conc.:
25 µg per animal
Nominal / measured:
meas. (not specified)
Conc. based on:
act. ingr.
Basis for effect:
mortality
Duration:
48 h
Dose descriptor:
LD50
Effect conc.:
> 25 µg per animal
Nominal / measured:
meas. (not specified)
Conc. based on:
act. ingr.
Basis for effect:
mortality
Details on results:
- Mortality at end of exposure period: zero
- Morphological abnormalities: none
- Behavioural abnormalities: none

Under the conditions of this study, there was no evidence of harmful effects or increased mortality caused by acute contact with AgSil ¿ 25.

Endpoint:
toxicity to terrestrial arthropods: short-term
Type of information:
read-across based on grouping of substances (category approach)
Adequacy of study:
key study
Justification for type of information:
Refer to category justification provided in IUCLID section 13.
Reason / purpose:
read-across source
Application method:
contact
Duration:
48 h
Dose descriptor:
LD0
Effect conc.:
25 µg per animal
Nominal / measured:
meas. (not specified)
Conc. based on:
act. ingr.
Basis for effect:
mortality
Remarks on result:
other:
Remarks:
Source, key, 1312-76-1, Richards 2006
Duration:
48 h
Dose descriptor:
LD50
Effect conc.:
> 25 µg per animal
Nominal / measured:
meas. (not specified)
Conc. based on:
act. ingr.
Basis for effect:
mortality
Remarks on result:
other:
Remarks:
Source, key, 1312-76-1, Richards 2006

Description of key information

Key value for chemical safety assessment

Additional information

No test available on sodium silicate. Data considered here is read-across from potassium silicate based on an analogue-approach. In a GLP guideline study with honey bees by Richards (2006), following EPA OPPTS 850.3020 (Honey Bee Acute Contact Toxicity), for a potassium silicate solution (29.1 wt %) the 48 h LD0 and L50 were determined to be 25 ug and > 25 ug active matter per animal, respectively.