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EC number: 204-289-6 | CAS number: 118-96-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Endpoint summary
Administrative data
Description of key information
Justification for selection of acute toxicity – oral endpoint
Only one study available.
Justification for selection of acute toxicity – inhalation endpoint
GLP study report available. Only one study available.
Justification for selection of acute toxicity – dermal endpoint
In accordance with column 2 of REACH Annex VIII, the testing by the
dermal route is not appropriate if inhalation of the substance is likely.
Key value for chemical safety assessment
Acute toxicity: via oral route
Link to relevant study records
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 401 (Acute Oral Toxicity)
- GLP compliance:
- no
- Remarks:
- No information on use of OECD and EU guidelines nor GLP conditions. The study was probably conducted according to TSCA guidelines
- Test type:
- standard acute method
- Species:
- rat
- Strain:
- Sprague-Dawley
- Sex:
- male/female
- Route of administration:
- oral: feed
- Vehicle:
- corn oil
- Doses:
- 1 ml per 100 g of body weight
- No. of animals per sex per dose:
- 4 dose levels were used, and 10 males and 10 females received each dose
- Sex:
- male
- Dose descriptor:
- LD50
- Effect level:
- ca. 1 320 mg/kg bw
- 95% CL:
- 95
- Sex:
- female
- Dose descriptor:
- LD50
- Effect level:
- ca. 795 mg/kg bw
- 95% CL:
- 95
- Interpretation of results:
- Category 4 based on GHS criteria
- Conclusions:
- Harmful if swallowed. According to Regulation (EC) No. 1272/2008 (EU CLP).
Reference
The LD50s and confidence intervals were calculated by the method of Thomson and Weil (Weil, 1952).
The effects of short-term exposure to alpha-TNT (up to 4 weeks) appear to be almost totally reversible. At the end of a 4 week period, the body weights of rats at the high dose level were still slightly lower (not significantly) than those of controls, and there were lingerinig signs of overcompensation to the anemia in females receiving 0.25% alpha-TNT. Thus at 0.05% alpha-TNT, liver-to-blood weight ratio in females remained altered (in an apparently dose-related manner) at the end of the recovery period. At 0.25% alpha-TNT, the digns of residual toxicity were clearer. At the end od the 4 week cerovery period, the body weights of females remained significantly depressed, they still had signs of anemia, and their spleens and livers remained enlarged. After the recovery period, the testes and kidney weights of males were lower than those of controls, and there was slightl granulocytosis in the males. these factors-the cumulative nature of alpha-TNT toxicity and more long-lasting effects after longer exposure periods for some parameters - are design considerations for longer term studies to assess hazards to humans.
Endpoint conclusion
- Endpoint conclusion:
- adverse effect observed
- Dose descriptor:
- LD50
- Value:
- 795 mg/kg bw
Acute toxicity: via inhalation route
Link to relevant study records
- Endpoint:
- acute toxicity: inhalation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2012
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 403 (Acute Inhalation Toxicity)
- GLP compliance:
- yes (incl. QA statement)
- Test type:
- standard acute method
- Species:
- rat
- Strain:
- Crj: CD(SD)
- Sex:
- male/female
- Route of administration:
- inhalation: aerosol
- Type of inhalation exposure:
- nose only
- Vehicle:
- air
- Analytical verification of test atmosphere concentrations:
- no
- Remarks:
- The carrier air into the exposure chamber and the extraction from it was monitored visually using calibrated flow meters and recorded at approximately 30 minute intervals throughout the exposure period.
- Duration of exposure:
- 4 h
- Concentrations:
- The target concentration of 1.0 mg/L was selected based on the known oral toxicity (LD50) of 2,4,6-Trinitrotoluene.
- No. of animals per sex per dose:
- 5 males, 5 females
- Control animals:
- no
- Key result
- Sex:
- male/female
- Dose descriptor:
- LC50
- Effect level:
- > 1.01 mg/L air
- Based on:
- test mat.
- Exp. duration:
- 4 h
- Mortality:
- There was no mortality observed during the study period.
- Clinical signs:
- other: Slow respiration, salivation and red liquid apparent in restraint tube were noted in all animals during the 4 hour exposure. Subdued behaviour, laboured respiration, rolling gait, piloerection, unkempt coat, and red staining, abdomen were noted post expos
- Body weight:
- All animals’ body weight profile was satisfactory throughout the study period
- Gross pathology:
- No necropsy findings were noted in any animal
- Interpretation of results:
- Category 3 based on GHS criteria
- Remarks:
- Based on harmonized classification.
- Conclusions:
- In conclusion, following a single 4 hour inhalation exposure to 2,4,6-Trinitrotoluene via snout only method no mortality or overt toxicity was observed during exposure or post exposure observation period. The LC50 is considered to be in excess of 1.01 mg/L.
- Executive summary:
Based on study results the substance meets category 4 criteria for dust/mist. However, the result is a boardline with harmonized classification and worst case scenario is applied.
Reference
The following parameters and end points were evaluated in this study: clinical signs, body weights, body weight changes, and gross necropsy findings.
In conclusion, following a single 4 hour inhalation exposure to 2,4,6-Trinitrotoluene via snout only method no mortality or overt toxicity was observed during exposure or post exposure observation period. The LC50 is considered to be in excess of 1.01 mg/L.
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed
- Dose descriptor:
- LC50
- Value:
- 1.01 mg/m³ air
Acute toxicity: via dermal route
Link to relevant study records
- Endpoint:
- acute toxicity: dermal
- Data waiving:
- study scientifically not necessary / other information available
- Justification for data waiving:
- the study does not need to be conducted because inhalation of the substance is likely
Reference
Endpoint conclusion
- Endpoint conclusion:
- no study available
Additional information
Justification for classification or non-classification
According to classification criteria Regulation (EC) No. 1272/2008 (EU CLP) and LD50 values, the substance is toxic if swallowed.
According to classification criteria Regulation (EC) No. 1272/2008 (EU CLP) and LC50 values, the substance is harmful if inhaled. However, taking into consideration harmonised classification and the possible exposure to human the substance can still be classfied as toxic if inhaled.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.

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