2,4,6-trinitrotoluene

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

comparable to guideline study

Data source

Reference

Reference Type:

publication

Title:

Unnamed

Year:

1982

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 401 (Acute Oral Toxicity)

GLP compliance:

no

Remarks:

No information on use of OECD and EU guidelines nor GLP conditions. The study was probably conducted according to TSCA guidelines

Test type:

standard acute method

Test material

Test material information

Constituent 1

Reference substance name:

2,4,6-trinitrotoluene

EC Number:

204-289-6

EC Name:

2,4,6-trinitrotoluene

Cas Number:

118-96-7

Molecular formula:

C7H5N3O6

IUPAC Name:

2-methyl-1,3,5-trinitrobenzene

Details on test material:

alpha-TNT was obtained from E.I. du Pont de Nemours. The compound was >99% pure based on chemical and chromatographic analyses.

Test animals

Species:

rat

Strain:

Sprague-Dawley

Sex:

male/female

Administration / exposure

Route of administration:

oral: feed

Vehicle:

corn oil

Doses:

1 ml per 100 g of body weight

No. of animals per sex per dose:

4 dose levels were used, and 10 males and 10 females received each dose

Results and discussion

Effect levels

Effect levels 1

Sex:

male

Dose descriptor:

LD50

Effect level:

ca. 1 320 mg/kg bw

95% CL:

95

Effect levels 2

Sex:

female

Dose descriptor:

LD50

Effect level:

ca. 795 mg/kg bw

95% CL:

95

Any other information on results incl. tables

The LD50s and confidence intervals were calculated by the method of Thomson and Weil (Weil, 1952).

The effects of short-term exposure to alpha-TNT (up to 4 weeks) appear to be almost totally reversible. At the end of a 4 week period, the body weights of rats at the high dose level were still slightly lower (not significantly) than those of controls, and there were lingerinig signs of overcompensation to the anemia in females receiving 0.25% alpha-TNT. Thus at 0.05% alpha-TNT, liver-to-blood weight ratio in females remained altered (in an apparently dose-related manner) at the end of the recovery period. At 0.25% alpha-TNT, the digns of residual toxicity were clearer. At the end od the 4 week cerovery period, the body weights of females remained significantly depressed, they still had signs of anemia, and their spleens and livers remained enlarged. After the recovery period, the testes and kidney weights of males were lower than those of controls, and there was slightl granulocytosis in the males. these factors-the cumulative nature of alpha-TNT toxicity and more long-lasting effects after longer exposure periods for some parameters - are design considerations for longer term studies to assess hazards to humans.

Applicant's summary and conclusion

Interpretation of results:

Category 4 based on GHS criteria

Conclusions:

Harmful if swallowed. According to Regulation (EC) No. 1272/2008 (EU CLP).