Phenethylamine

Toxicity to microorganisms

Administrative data

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Basic data given (documentation falls short of recent requirements, nevertheless sufficient information are available to assess toxic effect)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1987

Report date:

1987

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

DIN 38412-8 (Pseudomonas Zellvermehrungshemmtest)

Principles of method if other than guideline:

Growth inhibition test according to Bringmann and Kuehn

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Phenethylamine

EC Number:

200-574-4

EC Name:

Phenethylamine

Cas Number:

64-04-0

Molecular formula:

C8H11N

IUPAC Name:

2-phenylethanamine

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Pseudomonas putida

Details on inoculum:

Strain: DSM 50026

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

17 h

Test conditions

Test temperature:

20.0 °C

Nominal and measured concentrations:

Nominal test concentrations (not neutralised): 0 (control), 100, 200, 300, 400 and 500 mg/L

Details on test conditions:

Sample treatment: The stock solution (625 mg/L) was stirred for ca. 10 minutes.

TEST SYSTEM
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Nutrients provided for bacteria: 0.5 ml nutrient solution I (20-fold); 0.5 ml nutrient solution II (20-fold)
- Biomass loading rate: 1 ml bacteria suspension with NaCl (cell density: 10 TE/F)
- solution containing test substance: 8 ml
OTHER TEST CONDITIONS
- Adjustment of pH: no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

17 h

Dose descriptor:

EC10

Effect conc.:

242.5 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Effect concentrations 2

Duration:

17 h

Dose descriptor:

EC50

Effect conc.:

270 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Effect concentrations 3

Duration:

17 h

Dose descriptor:

other: EC90

Effect conc.:

297.5 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Any other information on results incl. tables

Extinction values (E 436 nm)

Nominal concentration [mg/L]	Extinction (inoculated)	Extinction (blank)	Difference	% of control
0 (control)	0.54	-	-	-
100	0.765	-0.005	0.77	142.66
200	0.817	-0.002	0.819	151.74
300	0.027	-0.008	0.035	6.44
400	0.006	-0.008	0.014	2.5
500	0.003	-0.008	0.011	2.08