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Administrative data

Endpoint:
water solubility
Type of information:
experimental study
Adequacy of study:
key study
Study period:
Experimental Starting Date: 09 June 2014 Experimental Completion Date: 22 August 2014
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: see 'Remark'
Remarks:
Study conducted in compliance with agreed protocols, with no or minor deviations from standard test guidelines and/or minor methodological deficiencies, which do not affect the quality of the relevant results. The study report was conclusive, done to a valid guideline and the study was conducted under GLP conditions.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2014
Report date:
2014

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to guideline
Guideline:
EU Method A.6 (Water Solubility)
Deviations:
no
Qualifier:
according to guideline
Guideline:
OECD Guideline 105 (Water Solubility)
Deviations:
no
GLP compliance:
yes
Type of method:
flask method
Remarks:
The determination was carried out using a flask method, designed to be compatible with Method A6 Water Solubility of Commission Regulation (EC) No 440/2008 of 30 May 2008 and Method 105 of the OECD Guidelines for Testing of Chemicals, 27 July 1995.

Test material

Constituent 1
Reference substance name:
1.25H Farnesene
IUPAC Name:
1.25H Farnesene
Test material form:
other: Clear colorless liquid
Details on test material:
Identification: 1.25H Farnesene
Appearance/Physical State: Clear colorless liquid
Lot: RV-752-153-2
Purity: 98.92%
Expiry Date: 01 March 2015
Storage Conditions: Approximately 4 °C, in the dark

Results and discussion

Water solubility
Water solubility:
<= 0 g/L
Temp.:
20 °C

Any other information on results incl. tables

Results

Preliminary Test:

The preliminary estimate of water solubility was 3.16 x 10-6 g/L at 20 °C.

Main Test:

Please see Attachment 1 for Typical Chromatography.

The mean peak areas relating to the standard and sample solutions are shown in the following tables:

Table 3.2 – Analysis of samples 1 and 3

 

Solution

Mean PeakArea

Standard at 0.104 mg/L

4.424 x 104

Standard at 0.112 mg/L

4.858 x 104

Sample 1A

5.327 x 104

Sample 1B

5.836 x 104

Sample 3A

7.467 x 104

Sample 3B

7.466 x 104

Standard at 0.104 mg/L

5.531 x 104

Standard at 0.112 mg/L

5.666 x 104

Table 3.3 – Analysis of sample 2

 

Solution

Mean Peak Area

Standard at 0.105 mg/L

1.041 x 105

Standard at 0.104 mg/L

9.494 x 104

Sample 2A

1.136 x 105

Sample 2B

1.185 x 105

Standard at 0.105 mg/L

1.030 x 105

Standard at 0.104 mg/L

9.518 x 104

 

The concentration (g/L) of test item in the sample solutions is shown in the following table:

Table 3.4

 

Sample Number

Time Shaken at

~ 30 ºC (h)

Time Equilibrated at 20 ºC (h)

Concentration

(g/L)

Solution pH

1

24

24

2.94 x 10-6

5.66

2

48

24

3.05 x 10-6

5.78

3

72

24

3.94 x 10-6

5.81

 


The sample concentrations (g/L) were then corrected by the recovery of analysis result (86.4%, see the validation section) for the sample procedure at a nominal concentration level of 2.5 x 10-6g/L.

 

Table 3.5

 

Sample Number

Concentration

Corrected by Recovery

(g/L)

1

3.41 x 10-6

2

3.53 x 10-6

3

4.56 x 10-6

 

Limit concentration    :          ≤4.56 x 10-6 g/L at 20.0 ± 0.5 ºC

Validation

The linearity of the detector response with respect to concentration was assessed over the nominal concentration range of 5 x 10-2 to 0.2 mg/L. This was satisfactory with a correlation coefficient of 1.000 being obtained.

 

Recovery of analysis for the sample procedure was assessed and proved adequate for the test. The recovery data is shown in the following table:

 

Table 3.6

 

Nominal Concentration

(g/L)

Recovery Range (%)

Mean Recovery (%)

1.0 x 10-5

81.1 to 87.3

84.0

5.0 x 10-6

87.4 to 97.0

90.8

2.5 x 10-6

79.8 to 93.8

86.4

 

 

Applicant's summary and conclusion

Conclusions:
Interpretation of results (migrated information): insoluble (< 0.1 mg/L)
Please see the Executive Summary below for the Conclusion.
Executive summary:
The determination was carried out using a flask method, designed to be compatible with Method A6 Water Solubility of Commission Regulation (EC) No 440/2008 of 30 May 2008 and Method 105 of the OECD Guidelines for Testing of Chemicals, 27 July 1995.

 

The test system consists of purified water with a resistivity of greater than 15MΩ.

        

Conclusion

The water solubility of the test item has been determined to be less than or equal to 4.56 x 10-6 g/L of solution at 20.0 ± 0.5 °C.