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Environmental fate & pathways

Biodegradation in water: screening tests

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Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: see 'Remark'
Remarks:
Study conducted in compliance with agreed protocols, with no or minor deviations from standard test guidelines and/or minor methodological deficiencies, which do not affect the quality of the relevant results. The study report was conclusive and done to a valid guideline.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2014
Report date:
2014

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
Deviations:
no
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Reaction mass of (6E)-2,6-dimethyl-10-methylenedodeca-2,6-diene and (6E)-3,7,11-trimethyldodeca-1,6,10-triene and (6E,10E)-2,6,10-trimethyldodeca-2,6,10-triene and (6E,10Z)-2,6,10-trimethyldodeca-2,6,10-triene
EC Number:
942-471-3
Cas Number:
1581740-29-5
Molecular formula:
C15H26
IUPAC Name:
Reaction mass of (6E)-2,6-dimethyl-10-methylenedodeca-2,6-diene and (6E)-3,7,11-trimethyldodeca-1,6,10-triene and (6E,10E)-2,6,10-trimethyldodeca-2,6,10-triene and (6E,10Z)-2,6,10-trimethyldodeca-2,6,10-triene
Test material form:
other: liquid
Details on test material:
- Name of test material (as cited in study report):RV-873-5-2 "Amyris Renewable Solvent"
- Physical state: Liquid
- Analytical purity: 98.37%
- Lot/batch No.: RV-873-5-2

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
other: Surface water within 1 mile of water treatment facility
Details on inoculum:

- inoculum: Surface water from Skokie, IL water district.
- proportion and nature of industrial waste water in sewage: unknown, discharge from waste treatment facility within 1
mile.
- test duration and temperature: 28 days or as indicated, 22C +/- 1C
- bacterial inoculum ~1E5 cfu/ml
Duration of test (contact time):
ca. 28 d
Initial test substance concentration
Initial conc.:
ca. 20 mg/L
Based on:
other: Total carbon determined by catalytic oxidation fo the test material
Parameter followed for biodegradation estimation
Parameter followed for biodegradation estimation:
other: Theoretical oxygen demand
Details on study design:
Study was conducted with 2 replicates.

SAMPLING
- Sampling frequency: continuous; respirometer



CONTROL: Sodium acetate

STATISTICAL METHODS: Sample results are provided as a graph showing the raw data and curvefit analysis.
Determinations of the percent degradation is based on the curve fit analysis performed with guidance from OECD Guidelines for the Testing of
Chemicals / Section 3: Degradation and Accumulation Test No. 314: Simulation Tests to Assess the Biodegradability of Chemicals Discharged in
Wastewater.
Reference substance
Reference substance:
acetic acid, sodium salt

Results and discussion

Preliminary study:
na
% Degradation
Parameter:
other: Theoretical oxygen demand
Value:
ca. 99
St. dev.:
10
Sampling time:
28 d
Remarks on result:
other: 10 day window requirement met
Details on results:
Achieved the requirements for Ready Biodegradability by the OECD 301F standard. Achieved a biodegradation plateau by day 28 of the test
period. By curve fit analysis, the achieved plateau was 99% ThO2 +/- SE 10%. the required degradation within the 10-day window was achieved.
OECD 301 ultimate degradation requirement achieved; 60% by day 19

BOD5 / COD results

Results with reference substance:
OECD 301 requirements achieved for sodium acetate; 10 day window (day 3 to day 12)
At 28 days

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Interpretation of results:
readily biodegradable
Conclusions:
Test material was readily biodegradable according to results of OECD 301F study.
Executive summary:

Myralene was tested via OECD 301F. Sodium acetate was also tested as a control. The inoculum used was surface water. 60% ThOD biodegradation was achieved by day 19 and within the requisite 10 day window. After 28 days, 99% ThOD was acheived. Test material was readily biodegradable according to results of OECD 301F study.