Registration Dossier
Registration Dossier
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 429-900-5 | CAS number: 82356-51-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Toxicity to soil macroorganisms except arthropods
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to soil macroorganisms except arthropods: short-term
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From 2001-09-13 to 2001-12-19
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Remarks:
- Experiment conducted under GLP, following OECD Guidelines. No deviations were observed.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 207 (Earthworm, Acute Toxicity Tests)
- Deviations:
- no
- Principles of method if other than guideline:
- Not applicable.
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Inspected on 28 February 2000. Signed on 26 April 2000
- Analytical monitoring:
- no
- Details on sampling:
- - Concentrations: 100, 180, 320, 560, and 1000 mg/kg
- Sampling method: At the start of the test, 10 earthworms were placed in each test and control vessel on the artificial soil, at random. The test vessels were covered with plastic film with ventilation holes and maintained in a temperature controlled room at 21°C under continuous illumination at 548 to 720 lux. The earthworms were not individually identified and received no food during exposure. Any mortalities and sub-lethal effects of exposure were recorded 7, and 14 days after the start of treatment. The criterion of death was taken to be the absence of any reaction to a physical stimulus at either end of the body. Any unaccounted for worms were classed as mortalities.
- Sample storage conditions before analysis: none - Vehicle:
- no
- Details on preparation and application of test substrate:
- - Method of mixing into soil (if used): Amount of test material were each separately incorporated into 2.5 kg (dry weight) of artificial soil with 650 mL of deionised reverse osmosis water and mixed using a Hobart QE 200 mixer to give the test concentrations with a nominal moisture content of 30% of dry weight. The control was prepared in an identical manner without the addition of test material.
- Controls: An amount of soil was taken for moisture content determination from the control and each test group. The remaining soil was dispersed to four replicates R1-R4. - Test organisms (species):
- Eisenia fetida
- Animal group:
- annelids
- Details on test organisms:
- TEST ORGANISM
- Common name: earthworms
- Source: Blades Biological, Edenbridge (Kent, UK)
- Age at test initiation (mean and range, SD): no data
- Weight at test initiation (mean and range, SD): 0.38 +/- 0.07 g - Study type:
- laboratory study
- Substrate type:
- artificial soil
- Limit test:
- no
- Total exposure duration:
- 14 d
- Remarks:
- none
- Post exposure observation period:
- no data
- Test temperature:
- 21°C
- pH:
- 5.7-6.0
- Moisture:
- 27-30 % of dry weight
- Details on test conditions:
- TEST SYSTEM
- Test container (material, size): 1 L glass beaker
- Amount of soil or substrate: 0.5 kg dry weight of substrate
- No. of organisms per container (treatment): 10 earthworms
- No. of replicates per treatment group: 1
- No. of replicates per control: 1
- No. of replicates per vehicle control: not applicable
OTHER TEST CONDITIONS
- Photoperiod: 24:0
- Light intensity: 483-705 lux - Nominal and measured concentrations:
- - nominal concentrations: 0, 100, 180, 320, 560, and 1000 mg/Kg
- No measured concentration. - Reference substance (positive control):
- yes
- Remarks:
- chloroacetamide (Sigma Batch No 70K0053)
- Key result
- Duration:
- 14 d
- Dose descriptor:
- LC50
- Effect conc.:
- 250 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Remarks on result:
- other: 95% CL
- Remarks:
- 240-260 mg/kg
- Duration:
- 14 d
- Dose descriptor:
- other: Highest concentration without observed effect
- Effect conc.:
- 180 mg/kg soil ww
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Remarks on result:
- not determinable
- Details on results:
- no data
- Results with reference substance (positive control):
- - Results with reference substance valid? yes
- Relevant effect levels: 14d-LC50 of 23 mg/Kg (95% confidence limits = 20 -25 mg/Kg).
- Other: none - Reported statistics and error estimates:
- no data
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 14d-LC50 for the test material to earthworms based on nominal test concentrations was 250 mg/Kg (95% confidence limits = 240 -260 mg/Kg).
- Executive summary:
A study, according to OECD Guideline 207 and EEC Commission Directive 87/302/EEC, was performed to assess the acute toxicity of the test material to the earthworm (Eisenia foetida) in an artificial soil.
Following a preliminary range-finding test (test concentrations of 10, 100 and 1000 mg/Kg), earthworms were exposed in groups of 40 (4 replicates of 10 worms per concentration) to a range of concentrations of 100, 180, 320, 560 and 1000 mg/Kg of soil for a period of 14 days. The number of mortalities was determined after 7 and 14 days exposure.
A positive control study using chloroacetamide, conducted approximately every 6 months, was reported for reference purposes.
The 14d-LC50 for the test material to earthworms based on nominal test concentrations was 250 mg/Kg (95% confidence limits = 240 -260 mg/Kg).
The result of the positive control study gave a 14d-LC50 for chloroacetamide of 23 mg/Kg (95% confidence limits = 20 -25 mg/Kg).
Reference
Table 6.3.1/1: Cumulative mortality in the definitive test.
Nominal concentration (mg/Kg) |
Cumulative mortality (initial population : 40) |
% Mortality |
||
|
Day 7 |
Day14 |
Day 7 |
Day 14 |
Control |
0 |
0 |
0 |
0 |
100 |
0 |
0 |
0 |
0 |
180 |
0 |
0 |
0 |
0 |
320 |
38 |
38 |
95 |
95 |
560 |
40 |
40 |
100 |
100 |
1000 |
40 |
40 |
100 |
100 |
Description of key information
OECD Guideline 207, GLP, key study, validity 1:
14d-LC50 (Eisenia fetida) = 250 mg/Kg (95% confidence limits = 240 -260 mg/Kg) based on nominal concentrations
Key value for chemical safety assessment
- Short-term EC50 or LC50 for soil macroorganisms:
- 250 mg/kg soil dw
Additional information
One key study is available, according to OECD Guideline 207 and EEC Commission Directive 87/302/EEC, to assess the acute toxicity of the registered substance to the earthworm (Eisenia fetida) in an artificial soil.
Following a preliminary range-finding test (test concentrations of 10, 100 and 1000 mg/Kg), earthworms were exposed in groups of 40 (4 replicates of 10 worms per concentration) to a range of concentrations of 100, 180, 320, 560 and 1000 mg test substance/Kg of soil for a period of 14 days. The number of mortalities was determined after 7 and 14 days exposure.
A positive control study using chloroacetamide, conducted approximately every 6 months, was reported for reference purposes. The result of the positive control study gave a 14d-LC50 for chloroacetamide of 23 mg/Kg (95% confidence limits = 20 -25 mg/Kg).
The 14d-LC50 for the test material to earthworms based on nominal test concentrations was 250 mg/Kg (95% confidence limits = 240 -260 mg/Kg).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
