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A GLP-compliant study according to OECD Guideline 203 with guppies (Poecilia reticulata) was available (Duphar B.V., 1992b). The 96-h LC50 was 560 mg/L in fish.

A GLP-compliant 48-h immobilisation test withDaphnia magna was performed according to OECD Guideline 202 (Duphar B.V., 1992c). The 48-h EC50 was 520 mg/L in Daphnia magna.

For algae a GLP-compliant growth inhibition test was performed according to OECD Guideline 201 (Duphar B.V., 1992d). The 96-h ErC50 was >1,000 mg/L and the 96-h NOErC was 250 mg/L inPseudokirchneriella subcapitata (tested as:Selenastrum capricornutum).

Long-term studies with fish and invertebrates are not available.

For the toxicity of microorganisms, a growth inhibition study is available withPseudomonas putida. At the nominal limit concentration of 1000 mg /L, no growth inhibition was observed. Therefore, it is concluded that the 3-h EC50 value is >1000 mg/L.