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Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
migrated information: read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Non-GLP guideline study according to German National Standard (DIN).

Data source

Referenceopen allclose all

Reference Type:
study report
Title:
Unnamed
Year:
1989
Report date:
1989
Reference Type:
other company data
Title:
Unnamed
Year:
2009
Report date:
2009

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: DIN 38412 part 9
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
2,6-dimethylmorpholine
EC Number:
205-509-3
EC Name:
2,6-dimethylmorpholine
Cas Number:
141-91-3
Molecular formula:
C6H13NO
IUPAC Name:
2,6-dimethylmorpholine
Details on test material:
- Name of test material (as cited in study report): 2,6 Dimethylmorpholin (cis/trans isomere mixture)
- Analytical purity: 98.6% (75.3% cis, 23.3% trans)
- Impurities (identity and concentrations): 1.2% water; 0.2% unknown impurities

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
Details on test organisms:
TEST ORGANISM
- Strain: SAG 86.81
- Method of cultivation: in OECD medium

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h

Test conditions

Test temperature:
20 °C
pH:
8.1 - 10.2
Nominal and measured concentrations:
Nominal: 0.98, 1.95, 3.91, 7.81, 15.63, 31.25, 62.5, 125, 250 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel:
- Material, fill volume: Erlenmeyer glass flasks, 250 ml
- Aeration: none
- Initial cells density: 10000 cells/ml
- No. of vessels per concentration (replicates): 5
- No. of vessels per control (replicates): 5

GROWTH MEDIUM
- Standard medium used: yes(OECD medium)

EFFECT PARAMETERS MEASURED: measured after 0 ,24 ,48, 72, 96 hours
- Determination of cell concentrations: fluorimeter

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2

Results and discussion

Effect concentrationsopen allclose all
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
218.945 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Remarks on result:
other: recalculated value; 95% CL: 193.568 - 256.771 mg/L
Duration:
72 h
Dose descriptor:
EC10
Effect conc.:
79.333 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Remarks on result:
other: recalculated value; 95% CL: 54.691 - 98.471 mg/L

Applicant's summary and conclusion

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