2,6-dimethylmorpholine

Toxicity to microorganisms

Administrative data

Endpoint:

toxicity to microorganisms

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Non-GLP guideline study according to the German National Standard (DIN).

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1988

Report date:

1988

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

DIN 38412-8 (Pseudomonas Zellvermehrungshemmtest)

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

2,6 Dimethylmorpholin (cis/trans isomere mixture)

IUPAC Name:

2,6 Dimethylmorpholin (cis/trans isomere mixture)

Details on test material:

- Name of test material (as cited in study report): 2,6 Dimethylmorpholin (cis/trans isomere mixture)
- Analytical purity: 98.6% (75.3% cis, 23.3% trans)
- Impurities (identity and concentrations): 1.2% water; 0.2% unknown impurities

Sampling and analysis

Analytical monitoring:

no

Test organisms

Test organisms (species):

Pseudomonas putida

Details on inoculum:

- Strain: DSM 50026

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

17 h

Test conditions

Test temperature:

24 ± 1 °C

pH:

4.9 - 9.4

Nominal and measured concentrations:

Nominal: 156.25, 312.5, 625, 1250, 2500, 5000, 7500 and 10000 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: 300 ml glass Erlenmeyer vessel
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Biomass loading rate: 1 mL of a Pseudomonas suspension adjusted to a turbidity of 10 TE/F

EFFECT PARAMETERS MEASURED: Photometric determination of light extinction after 17 hours of exposure

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: AK medium according to DIN 38412, part 8 (draft)

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

17 h

Dose descriptor:

EC10

Effect conc.:

730 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth inhibition

Effect concentrations 2

Duration:

17 h

Dose descriptor:

EC50

Effect conc.:

1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth inhibition

Effect concentrations 3

Duration:

17 h

Dose descriptor:

other: EC90

Effect conc.:

1 560 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth inhibition