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Short-term toxicity to fish

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Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
comparable to guideline study with acceptable restrictions
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Deviations:
yes
Remarks:
poorly documented
GLP compliance:
no
Specific details on test material used for the study:
Purity of each material was checked in the Organic Chemicals Synthesis Laboratory, AEQI, in Beltsville by gas chromatography: > 98%
Analytical monitoring:
not specified
Vehicle:
yes
Remarks:
Acetone
Test organisms (species):
other: Rainbow trout (cold water fish) and Bluegill (warm water fish)
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Nominal and measured concentrations:
5 concentrations from 3.2 to 10 mg/L.
Details on test conditions:
TEST SYSTEM
- Test vessel: Vessels were lined with disposable polyethylene bags
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: 12.5 L
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- No. of vessels per vehicle control (replicates): 1

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: 30 mg CaSO, 30 mg MgSO, 48 mg NaHCO, and 2 mg KC1 added per liter of deionized water.

EFFECT PARAMETERS MEASURED (with observation intervals if applicable): Deaths and/or untoward behavioral reactions.

TEST CONCENTRATIONS
Range finding study : Data not published.
Reference substance (positive control):
yes
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
6 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Remarks on result:
other: Rainbow trout
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
8.1 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Remarks on result:
other: Bluegill sunfish
Results with reference substance (positive control):
To check on suitability as test subjects, fish from each lot were challenged, under the same experimental conditions, with a reference pesticide (toxaphene, at 0.010, 0.018, and 0.056 ppm). The observed 96-hr LC50 was usually around 0.02 ppm; with one lot of bluegills there were no deaths at 0.018 ppm and no survivors at 0.056 ppm.
Validity criteria fulfilled:
not specified
Conclusions:
The 96h-LC50 was determined to be 6.0 mg/L for Rainbow trout and 8.1 mg/L for Bluegill sunfish.
Executive summary:

An acute toxicity test was perfomed on Rainbow trous (cold water fish) and Bluegill sunfish (warm water fish). Vessels were lined with disposable polyethylene bags and filled with 12.5 liter reconstituted water. Ten fish were placed in each vessel and allowed 24 h to become acclimated. The test item solutions in acetone, were then added to the bioassay vessels. Five concentrations were tested on the basis of preliminary screening tests. Control groups of fish in untreated water and in water to which acetone only was added were observed concurrently. The fish were observed for 96 h and all deaths and/or untoward behavioral reactions were recorded. The concentration of dissolved oxygen was measured in all solutions in which deaths occurred to be sure the test water contained sufficient oxygen; dissolved oxygen concentrations above 4 mg/L for warm-water fish (bluegills) or above 5 mg/L for cold-water fish (rainbow trout) were considered adequate. The median lethal concentrations (LC50) were calculated. The 96h-LC50 was determined to be 6.0 mg/L (4.9 -7.2 mg/L) for Rainbow trout and 8.1 mg/L (7.4 -9.0 mg/L) for Bluegill sunfish.

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2003
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study with acceptable restrictions
Qualifier:
according to guideline
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Deviations:
yes
Remarks:
poorly documented
GLP compliance:
not specified
Specific details on test material used for the study:
4-Allyl-1,2-dimethoxybenzene
Analytical monitoring:
yes
Vehicle:
not specified
Test organisms (species):
Oryzias latipes
Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Nominal and measured concentrations:
10, 18, 32, 56, 100 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel: 3L
- Renewal rate of test solution (frequency/flow rate): 48h
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1


OTHER TEST CONDITIONS
- Temperature: 24 ºC
Adjustment of pH: NO
- Photoperiod: 16h light / 8h dark

EFFECT PARAMETERS MEASURED (with observation intervals if applicable): Mortality
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
14.1 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Details on results:
The 96h-LC50 was determined to be 14.1 mg/L (95% CL: 11.8-16.5 mg/L) in Oryzias latipes.
Validity criteria fulfilled:
not specified
Conclusions:
The 96h-LC50 was determined to be 14.1 mg/L in Oryzias latipes.
Executive summary:

An acute fish test was performed according to the OECD Guideline 203 on Oryzias latipes. 10 fish per dose were exposed up to 100 mg/L test item for 96 hours under semi-static conditions. Test item was measured by HPLC analysis. The 96h-LC50 was determined to be 14.1 mg/L (95% CL: 11.8-16.5 mg/L) based on mortality.

Description of key information

Key study: Test method similar to OECD 203. The 96h-LC50 was determined to be 6.0 mg/L for Rainbow trout and 8.1 mg/L for Bluegill sunfish.

Key study: Test method OECD 203. The 96h-LC50 was determined to be 14.1 mg/L in Oryzias latipes.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish
Effect concentration:
6 mg/L

Additional information