4-allylveratrole

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2003

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

yes

Remarks:

poorly documented

GLP compliance:

not specified

Specific details on test material used for the study:

4-Allyl-1,2-dimethoxybenzene

Analytical monitoring:

yes

Vehicle:

not specified

Test organisms (species):

Daphnia magna

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Nominal and measured concentrations:

10, 18, 32, 56, 100 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: 100 mL
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 1

OTHER TEST CONDITIONS
- Temperature: 20 ± 1 ºC
- Adjustment of pH: No.
- Photoperiod:16 hours light/ 8 hours dark

EFFECT PARAMETERS MEASURED (with observation intervals if applicable): Mobility

Reference substance (positive control):

not specified

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

38.2 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

The 48h-EC50 was determined to be 38.2 mg/L (95% CL: 34.4-42.6 mg/L) in Daphnia magna.

Validity criteria fulfilled:

not specified

Conclusions:

The 48h-EC50 was determined to be 38.2 mg/L in Daphnia magna.

Executive summary:

An acute Daphnia magna immobilization test was performed according to the OECD Guideline 202. Five Daphnids per replicate were exposed up to 100 mg/L (20 Daphnids per dose) for 48 hours under static conditions. Test item concentrations were measured by HPLC analysis. The 48h-EC50 was determined to be 38.2 mg/L (95% CL: 34.4-42.6 mg/L) in Daphnia magna based on mobility.

Description of key information

Key study: Test method OECD 202. The 48h-EC50 was determined to be 38.2 mg/L in Daphnia magna.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

38.2 mg/L