4-allylveratrole

Long-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

long-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2003

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Qualifier:

according to guideline

Guideline:

OECD Guideline 211 (Daphnia magna Reproduction Test)

Deviations:

yes

Remarks:

poorly documented

GLP compliance:

not specified

Specific details on test material used for the study:

4-Allyl-1,2-dimethoxybenzene

Analytical monitoring:

yes

Vehicle:

not specified

Test organisms (species):

Daphnia magna

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

21 d

Nominal and measured concentrations:

0.32, 1.0, 3.2, 10, 32 mg/L

Details on test conditions:

TEST SYSTEM
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 10
- No. of vessels per control (replicates): 1

OTHER TEST CONDITIONS
- Temperature: 20 ± 1 ºC
- Adjustment of pH: No
- Photoperiod: 16 hours light/8 hours dark

EFFECT PARAMETERS MEASURED (with observation intervals if applicable): Reproduction

Reference substance (positive control):

not specified

Key result

Duration:

21 d

Dose descriptor:

EC50

Effect conc.:

13.4 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

reproduction

Key result

Duration:

21 d

Dose descriptor:

NOEC

Effect conc.:

1.06 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

reproduction

Details on results:

The 21d-EC50 was determined to be 13.4 mg/L (95% CL: 11.6-15.7 mg/) in Daphnia magna (based on reproduction).
The 21d-EC50 was determined to be 1.06 mg/L in Daphnia magna (based on reproduction).

Validity criteria fulfilled:

not specified

Conclusions:

The 21d-EC50 and 21d-NOEC were determined to be 13.4 mg/L and 1.06 mg/L respectively in Daphnia magna (based on reproduction).

Executive summary:

A Daphnia magna reproduction test was performed with the test item. 10 Daphnids per replicate and dose were exposed up to 32 mg/L test item for 21 days under semi-static conditions. Test item concentration was measured by HPLC analysis. The Reproduction of Daphnids were observed. The 21d-EC50 was determined to be 13.4 mg/L (95% CL: 11.6-15.7 mg/) and the 21d-EC50 was determined to be 1.06 mg/L (both based on reproduction).

Description of key information

Kety study: Test method OECD 211. The 21d-EC50 and 21d-NOEC were determined to be 13.4 mg/L and 1.06 mg/L respectively in Daphnia magna (based on reproduction).

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

1.06 mg/L