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Long-term toxicity to aquatic invertebrates

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Reference
Endpoint:
long-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2003
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
comparable to guideline study with acceptable restrictions
Qualifier:
according to guideline
Guideline:
OECD Guideline 211 (Daphnia magna Reproduction Test)
Deviations:
yes
Remarks:
poorly documented
GLP compliance:
not specified
Specific details on test material used for the study:
4-Allyl-1,2-dimethoxybenzene
Analytical monitoring:
yes
Vehicle:
not specified
Test organisms (species):
Daphnia magna
Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
21 d
Nominal and measured concentrations:
0.32, 1.0, 3.2, 10, 32 mg/L
Details on test conditions:
TEST SYSTEM
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 10
- No. of vessels per control (replicates): 1

OTHER TEST CONDITIONS
- Temperature: 20 ± 1 ºC
- Adjustment of pH: No
- Photoperiod: 16 hours light/8 hours dark

EFFECT PARAMETERS MEASURED (with observation intervals if applicable): Reproduction
Reference substance (positive control):
not specified
Key result
Duration:
21 d
Dose descriptor:
EC50
Effect conc.:
13.4 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
reproduction
Key result
Duration:
21 d
Dose descriptor:
NOEC
Effect conc.:
1.06 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
reproduction
Details on results:
The 21d-EC50 was determined to be 13.4 mg/L (95% CL: 11.6-15.7 mg/) in Daphnia magna (based on reproduction).
The 21d-EC50 was determined to be 1.06 mg/L in Daphnia magna (based on reproduction).
Validity criteria fulfilled:
not specified
Conclusions:
The 21d-EC50 and 21d-NOEC were determined to be 13.4 mg/L and 1.06 mg/L respectively in Daphnia magna (based on reproduction).
Executive summary:

A Daphnia magna reproduction test was performed with the test item. 10 Daphnids per replicate and dose were exposed up to 32 mg/L test item for 21 days under semi-static conditions. Test item concentration was measured by HPLC analysis. The Reproduction of Daphnids were observed. The 21d-EC50 was determined to be 13.4 mg/L (95% CL: 11.6-15.7 mg/) and the 21d-EC50 was determined to be 1.06 mg/L (both based on reproduction).

Description of key information

Kety study: Test method OECD 211. The 21d-EC50 and 21d-NOEC were determined to be 13.4 mg/L and 1.06 mg/L respectively in Daphnia magna (based on reproduction).

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
1.06 mg/L

Additional information