Registration Dossier

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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2015
Report date:
2015

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
1,1,2,3-tetramethyl-1H-benzo[e]indol-3-ium 4-methylbenzene-1-sulfonate
Cas Number:
141914-99-0
Molecular formula:
C23H25NO3S
IUPAC Name:
1,1,2,3-tetramethyl-1H-benzo[e]indol-3-ium 4-methylbenzene-1-sulfonate

Test organisms

Test organisms (species):
Daphnia magna

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h

Results and discussion

Effect concentrations
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
51 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Details on results:
Note that the EC50 was determined using nominal test concentrations. The analytical results were based on the quantification of both the anionic and the cationic parts of the test substance and showed that the test concentrations were in agreement with nominal and remained stable during the 48-hour test period. However, extra peaks were observed during analysis. In addition, at the end of the exposure period a slight orange/brown colouring and undissolved particles were observed at the highest test concentration.

Applicant's summary and conclusion