Phosphonium, [(2E)-5-[(4R)-4-hydroxy-2,6,6-trimethyl-1-cyclohexen-1-yl]-3-methyl-2-penten-4-ynyl]triphenyl-, chloride

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Remarks:

Type of genotoxicity: gene mutation

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: study performed according to OECD and GLP guidelines

Cross-reference

Reason / purpose for cross-reference:

reference to other study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1997

Report date:

1997

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 471 (Bacterial Reverse Mutation Assay)

GLP compliance:

yes (incl. QA statement)

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

Zeanyne salt

EC Number:

618-777-5

Cas Number:

91662-51-0

Molecular formula:

C33H36ClOP

IUPAC Name:

Zeanyne salt

Method

Target gene:

TA97: hisD6610, TA 98: hisD3052; TA 100 hisG46 TA102 hisG428 and TA 1535 hisG46

Species / strain

Species / strain / cell type:

other: TA97, TA 98, TA 100, TA102 and TA 1535

Additional strain / cell type characteristics:

other: rfa, delta-uvrB, pKM101, pAQ1

Metabolic activation:

with and without

Metabolic activation system:

S9

Test concentrations with justification for top dose:

0, 1, 3, 10, 33, 100, 333, 1000, 3333, 5000 ug/plate in the preliminary test,
2 to 200 ug/plate in the main experiment

Controls

Untreated negative controls:

yes

Negative solvent / vehicle controls:

yes

True negative controls:

no

Positive controls:

yes

Positive control substance:

2-nitrofluorene

sodium azide

mitomycin C

other: ICR 191, 2-Aminoanthracene

Results and discussion

Test results

Species / strain:

other: TA97, TA 98, TA 100, TA102 and TA 1535

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

not determined

Vehicle controls validity:

valid

Untreated negative controls validity:

valid

Positive controls validity:

valid

Remarks on result:

other: all strains/cell types tested

Remarks:

Migrated from field 'Test system'.

Any other information on results incl. tables

-

Applicant's summary and conclusion

Conclusions:

Interpretation of results (migrated information):
negative

Neither Zeanyne salt per se, nor any of the formed metabolites was mutagenic in the Ames test under the described experimental
conditions.

Executive summary:

Zeanyne salt was evaluated for mutagenic activity in the Ames test. A standard plate incorporation and a

preincubation modification assay in absence and in presence of an exogenous

metabolic activation system (S9) were performed. Five Salmonella typhimurium tester strains (TA1535, TA97, TA98, TA100, and TA102) were employed. The activity of the S9-mix and the responsiveness of the tester strains were verified by including

appropriate controls into each experiment.

The substance was dissolved in dimethylsulfoxide (DMSO). Toxic effects were observed in a preliminary toxicity experiment starting at 333 ug/plate (on VB plates).

Therefore the concentration range 2 to 200 pg/plate was evaluated in the main experiments. Upon addition to the aqueous medium no precipitation of the compound was observable. Strain dependent toxic effects were observed (reduction in the number

of revertants and/or reduction in the background growth). Using the preincubation

modification assay, known to be more sensitive for several class of compounds, the

toxic effects were more pronounced. No increase of the number of mutant colonies was apparent in any of the five investigated

tester strains. Thus it can be concluded, that neither Zeanyne salt per se, nor any of the formed

metabolites was mutagenic in the Ames test under the described experimental conditions.