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EC number: 942-993-1 | CAS number: -
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
Description of key information
ErL50 (algae; growth rate) = > 100 mg/L ; WAF loading 72hour-freshwater, OECD TG 201, Wil Research B.V. 2015
ErL10 (algae; growth rate)= > 100 mg/L ; WAF loading 72 hour-freshwater, OECD TG 201, Wil Research B.V. 2015
NOELR (algae; growth rate) = > 100 mg/L; WAF loading, 72 hour-freshwater, OECD TG 201, Wil Research B.V. 2015
Key value for chemical safety assessment
Additional information
The algal growth inhibition to Pseudokirchneriella subcapitata, was carried out according to OECD 201 Freshwater Alga and Cyanobacteria, Growth Inhibition Test and EU Method C.3 guidelines. All test substance solution concentrations were individually prepared and magnetically stirred for two days to ensure that maximum dissolution was obtained. This resulted in clear solutions all containing a floating layer of undissolved test item droplets. The Water Accommodated Fractions (WAFs) were subsequently collected after a three-hour stabilisation period. The final test solutions were all clear and colourless. A limit test was combined with a range-finding test. Six exponentially growing algal cultures were exposed to an untreated control and a WAF prepared at a loading rate of 100 mg/L in the limit test. In addition, three replicates per group were exposed to WAFs prepared at loading rates of 1.0 and 10 mg/L in a combined range-finding test. The initial algal cell density was 10^4 cells/mL. The total exposure period was 72 hours. No significant differences were recorded between the values for growth rate or yield at any of the test concentrations when compared to the control group. Samples for Total Organic Carbon (TOC) analyses were taken at the start and at the end of the exposure period. The study met the acceptability criteria prescribed and was considered valid. Under the conditions of the study with Pseudokirchneriella subcapitata, no inhibition of growth rate or inhibition of yield was recorded at any of the concentrations of the test item tested. The EL50 for growth rate inhibition (72h-ErL50) and the EL50 for yield inhibition (72h-EyL50) exceeded the loading rate of 100 mg/L. The 72h-NOELR for both growth rate inhibition and yield inhibition was greater than or equal to the loading rate of 100 mg/L. No EL50-values could be estimated because the test substance proved to be non-toxic (EL50 > maximum loading rate).
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