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Description of key information

Tin sulfide and tin disulfide had no sensitising effect in the murine local lymph node assay according to OECD guideline 429.

Based on this information it is considered that also ditin trisulfide reveals no sensitising effect.

Key value for chemical safety assessment

Skin sensitisation

Link to relevant study records
Reference
Endpoint:
skin sensitisation: in vivo (LLNA)
Type of information:
read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
key study
Justification for type of information:
Read-Across Justification_SnS and SnS2 to Sn2S3
Reason / purpose for cross-reference:
read-across source
Parameter:
other: clinical observations
Remarks:
72h after challenge
Value:
0
Test group / Remarks:
30% in DAE
Remarks on result:
other: SnS/No observations
Parameter:
SI
Remarks:
mean per treatment group
Value:
1.35
Test group / Remarks:
30% in DAE 433
Remarks on result:
other: SnS
Parameter:
SI
Remarks:
mean per treatment group
Value:
1.91
Test group / Remarks:
3% in DAE 433
Parameter:
SI
Remarks:
mean per treatment group
Value:
1.86
Test group / Remarks:
0.3% in DAE 433
Parameter:
SI
Remarks:
mean per treatment group
Value:
1
Test group / Remarks:
negative control
Parameter:
SI
Remarks:
mean per treatment group
Value:
16.94
Test group / Remarks:
positive control 0.5% DNCB
Parameter:
other: DPM: desintegrations per minute
Remarks:
mean per treatment group
Value:
319.4
Test group / Remarks:
30% in DAE 433
Parameter:
other: DPM: desintegrations per minute
Remarks:
mean per treatment group
Value:
452.9
Test group / Remarks:
3% in DAE 433
Parameter:
other: DPM: desintegrations per minute
Remarks:
mean per treatment group
Value:
441.5
Test group / Remarks:
0.3% in DAE 433
Parameter:
other: DPM: desintegrations per minute
Remarks:
mean per treatment group
Value:
237.3
Test group / Remarks:
negative control
Parameter:
other: DPM: desintegrations per minute
Remarks:
mean per treatment group
Value:
4 018.4
Test group / Remarks:
positive control 0.5% DNCB
Interpretation of results:
GHS criteria not met
Conclusions:
Tin sulfide had no sensitising effect in the murine local lymph node assay according to OECD guideline 429.
Based on this information it is considered that also ditin trisulfide reveals no sensitising effect.
Executive summary:

Read-across substance tin sulfide was assessed for skin sensitisation potential with the murine local lymph node assay (LLNA). The LLNA with radionuclides was performed according to OECD guideline 429.

The animals exposed to the test substance at all concentrations (30 %, 3 %, 0.3 % in DAE 433) showed no pathological skin reactions and no other negative clinical symptoms of intoxication throughout the experiment. There was no significant difference in body weight increment of all groups in comparison to the vehicle control. The positive control substance DNCB elicited a reaction pattern with statistically significant increase in ear weight and Stimulation Index of cell proliferation 16.9, which was in congruence with his expected mode of action as a contact allergen. The test substance tin sulfide did not show a tendency to increased ear weight in any of concentrations tested. The result of skin irritation effect was considered as negative - it means the test substance did not cause irritation of skin. Comparison of Stimulation Indexes between all treated groups and control vehicle group revealed that the test substance tin sulfide did not cause increase in radioisotope incorporation into the DNA of dividing lymphocytes. In conclusion, at the given experimental conditions the read-across test substance tin sulfide provided a negative result in LLNA test.

Based on this information it is considered that also ditin trisulfide reveals no sensitising effect.

Endpoint conclusion
Endpoint conclusion:
no adverse effect observed (not sensitising)
Additional information:

Read-across substance in sulfide was assessed for skin sensitisation potential with the murine local lymph node assay (LLNA). The LLNA with radionuclides was performed according to OECD guideline 429.

The animals exposed to the test substance at all concentrations (30 %, 3 %, 0.3 % in DAE 433) showed no pathological skin reactions and no other negative clinical symptoms of intoxication throughout the experiment. There was no significant difference in body weight increment of all groups in comparison to the vehicle control. The positive control substance DNCB elicited a reaction pattern with statistically significant increase in ear weight and Stimulation Index of cell proliferation 16.9, which was in congruence with his expected mode of action as a contact allergen. The test substance tin sulfide did not show a tendency to increased ear weight in any of concentrations tested. The result of skin irritation effect was considered as negative - it means the test substance did not cause irritation of skin. Comparison of Stimulation Indexes between all treated groups and control vehicle group revealed that the test substance tin sulfide did not cause increase in radioisotope incorporation into the DNA of dividing lymphocytes. In conclusion, at the given experimental conditions the test substance tin sulfide provided a negative result in LLNA test.

An in vitro or in chemico skin sensitisation study does not need to be conducted because adequate data from an in vivo skin sensitisation study were already available.

Justification for selection of skin sensitisation endpoint:

GLP and guideline compliant study on a structural analogous read-across substance

Respiratory sensitisation

Endpoint conclusion
Endpoint conclusion:
no study available

Justification for classification or non-classification

Based on the results on tin sulfide and tin disulfide in sensitisation studies no classification and labelling is required for ditin trisulfide according to Regulation (EC) No 1272/2008 (CLP) or Directive 67/548/EEC (DSD).