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EC number: 205-860-2 | CAS number: 156-60-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: see 'Remark'
- Remarks:
- Data taken from accepted publication with limited details on methods and results. This study was selected as the key study because the information provided for the hazard endpoint is sufficient for the purpose of classification and labelling and/or risk assessment.
Data source
Reference
- Reference Type:
- publication
- Title:
- Biodegradability studies with organic priority pollutant compounds
- Author:
- Tabak HH, Quave SA, Mashni CI, and Barth EF
- Year:
- 1 981
- Bibliographic source:
- J WPCF, 53(10):1503-1518
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)
- Deviations:
- yes
- Remarks:
- 3 bottles per concentration; volatile fraction also captured and measured
- GLP compliance:
- not specified
Test material
- Reference substance name:
- trans-dichloroethylene
- EC Number:
- 205-860-2
- EC Name:
- trans-dichloroethylene
- Cas Number:
- 156-60-5
- Molecular formula:
- C2H2Cl2
- IUPAC Name:
- (1E)-1,2-dichloroethene
- Details on test material:
- - Purity: not reported
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic (adaptation not specified)
- Details on inoculum:
- - Source of inoculum/activated sludge: settled domestic wastewater as microbial inoculum
- Duration of test (contact time):
- 28 d
Initial test substance concentrationopen allclose all
- Initial conc.:
- 5 mg/L
- Based on:
- test mat.
- Initial conc.:
- 10 mg/L
- Based on:
- test mat.
Parameter followed for biodegradation estimationopen allclose all
- Parameter followed for biodegradation estimation:
- test mat. analysis
- Parameter followed for biodegradation estimation:
- TOC removal
- Details on study design:
- TEST CONDITIONS
- Composition of medium: biochemical oxygen demand (BOD) dilution water containing 5 mg of yeast extract per litre, as the synthetic medium
- Test temperature: 25°C
- Continuous darkness: yes
TEST SYSTEM
- Culturing apparatus: 250-mL glass-stoppered reagent bottles
- Test performed in closed vessels due to significant volatility of test substance: yes
- Number of culture flasks/concentration: 3
SAMPLING
- Sampling frequency: every 7 days
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Abiotic sterile control: yes
- Other: Volatility controls, utilizing a nonbiological system (medium-test compound without inoculum) were held at both refrigerated and 25°C holding temperatures for 10 days and then analysed by GC and for TOC to determine loss of substrate from volatilization.
Reference substance
- Reference substance:
- other: Phenol
Results and discussion
% Degradationopen allclose all
- Parameter:
- % degradation (test mat. analysis)
- Value:
- 67
- Sampling time:
- 7 d
- Remarks on result:
- other: 5 mg/L test
- Parameter:
- % degradation (test mat. analysis)
- Value:
- 95
- Sampling time:
- 28 d
- Remarks on result:
- other: 5 mg/L test
- Parameter:
- % degradation (test mat. analysis)
- Value:
- 40
- Sampling time:
- 7 d
- Remarks on result:
- other: 10 mg/L test
- Parameter:
- % degradation (test mat. analysis)
- Value:
- 93
- Sampling time:
- 28 d
- Remarks on result:
- other: 10 mg/L test
- Parameter:
- % degradation (test mat. analysis)
- Value:
- 33
- Sampling time:
- 10 d
- Remarks on result:
- other: percent loss from 5 mg/L non-biologic control due to volatility
- Details on results:
- The test item exhibits slow biodegradative activity concomitant with relatively moderate rate of volatilization established in the non-biological volatility control systems. The test item was shown to be significantly biodegradable with gradual adaptation observed. The relatively significant volatilization in the non-biological volatility control series did not preclude the biodegradative activity of microbiota in culture media from the relatively high total percentage of loss of test item throughout the study in comparison to the volatility percentage losses.
BOD5 / COD results
- Results with reference substance:
- Phenol in the 5 and 10 mg/L positive control degraded 100% by Day 14.
Any other information on results incl. tables
|
Average total loss (% biodegradation) of test item in 7 day intervals |
Volatile Loss (%) 10 Days |
||||||
Test Item |
mg/mL |
Degradation summary |
Day 7 |
Day 14 |
Day 21 |
Day 28 |
5°C |
25°C |
Phenol (reference) |
5 |
D |
96 |
100 |
100 |
100 |
- |
- |
Phenol (reference) |
10 |
D |
97 |
100 |
100 |
100 |
- |
- |
Test Item |
5 |
B |
67 |
79 |
88 |
95 |
0 |
33 |
Test Item |
10 |
B |
40 |
76 |
81 |
93 |
0 |
26 |
D Significant degradation with rapid adaptation. B Slow to moderate biodegradative activity, concomitant with significant rate of volatilization. |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- other: slow biodegradative activity concomitant with relatively moderate rate of volatilization
- Conclusions:
- This study and the conclusions which are drawn from it fulfil the quality criteria (validity, reliability, repeatability).
The test item exhibits slow biodegradative activity concomitant with relatively moderate rate of volatilization. - Executive summary:
The biodegradability test method used in the study was the static-culture flask-screening procedure using 5- and 10- mg/L concentrations of the test item with a 7-day static incubation followed by three weekly subcultures (total incubation of 28 days. Volatility controls, utilizing a non-biological system (medium and test compound without inoculum) were held at both refrigerated and 25°C holding temperatures for 10 days and then analysed by GC and for TOC to determine loss of the test item from volatilization.
For the 5 mg/mL concentration the 28 day percent total loss of compound was 95%. The volatilization loss was 33%. In the 10 mg/mL test, total loss was 93% with a 26% loss due to volatilization. The test item exhibits slow biodegradative activity concomitant with relatively moderate rate of volatilization established in the non-biological volatility control systems. The test item was shown to be significantly biodegradable with gradual adaptation observed. The relatively significant volatilization in the non-biological volatility control series did not preclude the biodegradative activity of microbiota in culture media from the relatively high total percentage of loss of test item throughout the study in comparison to the volatility percentage losses.
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